Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,243,047	A→T	Y90N (TAT→AAT)	uxaA ←	D‑altronate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,243,047	0	A	T	100.0%	171.5 / NA	45	Y90N (TAT→AAT)	uxaA	D‑altronate dehydratase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (24/21);  total (24/21)

TAGCGCGACGATAGATCTGCACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATACTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCGTGAACGTGAACACCTGCCGCAATATCCGC  >  NC_000913/3242972‑3243116                                                                            |                                                                      tAGCGCGACGATAGATCTGCACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGAt                                                                  >  1:558727/1‑81 (MQ=255)  aGCGCGACGATAGATCTGCACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATc                                                                 >  1:558740/1‑81 (MQ=255)  aGCGCGACGATAGATCTGCACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATc                                                                 >  2:558731/1‑81 (MQ=255)   gcgcGACGATAGATCTGCACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATcc                                                                <  2:558680/81‑1 (MQ=255)   gcgcGACGATAGATCTGCACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATc                                                                 <  1:558681/80‑1 (MQ=255)    cgcgACGATAGATCTGCACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCa                                                               <  2:558682/81‑1 (MQ=255)       gacgaTAGATCTGCACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATc                                                                 >  2:558737/1‑76 (MQ=255)       gacgaTAGATCTGCACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGAt                                                            <  1:558683/81‑1 (MQ=255)         cgaTAGATCTGCACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCg                                                          >  2:558723/1‑81 (MQ=255)           aTAGATCTGCACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCt                                                        >  1:558724/1‑81 (MQ=255)           aTAGATCTGCACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCt                                                        <  1:558685/81‑1 (MQ=255)           aTAGATCTGCACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCt                                                        <  1:558684/81‑1 (MQ=255)               aTCTGCACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCt                                                        >  1:558728/1‑77 (MQ=255)                   gCACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCg                                                >  1:558736/1‑81 (MQ=255)                    cACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGAtt                                                  <  1:558686/78‑1 (MQ=255)                       ttCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTgcg                                            >  1:558726/1‑81 (MQ=255)                         cACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGcgcg                                          >  2:558741/1‑81 (MQ=255)                            gATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTAt                                       >  2:558729/1‑81 (MQ=255)                             aTCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTAtt                                      >  2:558763/1‑81 (MQ=255)                                tGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATtgtg                                   >  1:558734/1‑81 (MQ=255)                                  ccGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTgg                                  <  2:558687/80‑1 (MQ=255)                                   cGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGc                                <  1:558688/81‑1 (MQ=255)                                   cGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGc                                <  2:558689/81‑1 (MQ=255)                                    gCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGcg                               >  1:558749/1‑81 (MQ=255)                                     cTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGcgt                              >  2:558750/1‑81 (MQ=255)                                     cTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGcgt                              <  2:558690/81‑1 (MQ=255)                                        gcgcAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGcgtgaa                           >  1:558733/1‑81 (MQ=255)                                        gcgcAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGcgtg                             <  2:558691/79‑1 (MQ=255)                                          gcaggcagATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGcgtgaacg                         >  2:558746/1‑81 (MQ=255)                                            aggcagATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGcgtgaacgt                        >  1:558739/1‑80 (MQ=255)                                             ggcagATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGcgtgaacgtga                      <  2:558692/81‑1 (MQ=255)                                               cagATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCgtgaacgtgaac                    <  1:558693/81‑1 (MQ=255)                                                agATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCGTGAACGTGAaca                   >  1:558745/1‑81 (MQ=255)                                                 gATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCGTGAACGTGAacac                  <  2:558694/81‑1 (MQ=255)                                                   tCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCGTGAACGTGAACACCt                <  2:558697/81‑1 (MQ=255)                                                   tCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCGTGAACGTGAACACCt                <  1:558696/81‑1 (MQ=255)                                                   tCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCGTGAACGTGAACACCt                <  1:558695/81‑1 (MQ=255)                                                      tGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCGTGAACGTGAACACCTGcc             >  1:558753/1‑81 (MQ=255)                                                       gAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCGTGAACGTGAACACCTGCCg            >  2:558735/1‑81 (MQ=255)                                                       gAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCGTGAACGTGAACACCTGCCg            >  1:558747/1‑81 (MQ=255)                                                        aaaaTCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCGTGAACGTGAACACCTGcc             <  2:558698/79‑1 (MQ=255)                                                        aaaaTCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCGTGAACGTGAACACCTGCCg            >  1:558744/1‑80 (MQ=255)                                                        aaaaTCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCGTGAACGTGAACACCTGCCGc           <  1:558699/81‑1 (MQ=255)                                                          aaTCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCGTGAACGTGAACACCTGCCGCaa         <  1:558700/81‑1 (MQ=255)                                                                 ttGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCGTGAACGTGAACACCTGCCGCAATATCCGc  >  1:558760/1‑81 (MQ=255)                                                                            |                                                                      TAGCGCGACGATAGATCTGCACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATACTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCGTGAACGTGAACACCTGCCGCAATATCCGC  >  NC_000913/3242972‑3243116

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑