Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,300,767	T→G	V170V (GTA→GTC)	yhbT ←	SCP2 domain‑containing protein YhbT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,300,767	0	T	G	100.0%	150.1 / NA	41	V170V (GTA→GTC)	yhbT	SCP2 domain‑containing protein YhbT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (18/23);  total (18/23)

TCGAATGAGCGACGCAGCAGGGCATCAATACCCGGCGCATCAATGGGAATTTCTACTCGAATTAGCATGGTTCACCTACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATGCG  >  NC_000913/3300691‑3300844                                                                             |                                                                              tCGAATGAGCGACGCAGCAGGGCATCAATACCCGGCGCATCAATGGGAATTTCTACTCGAATTAGCATGGTTCACCGAcc                                                                            >  1:570759/1‑80 (MQ=255)  cGAATGAGCGACGCAGCAGGGCATCAATACCCGGCGCATCAATGGGAATTTCTACTCGAATTAGCATGGTTCAccgaccga                                                                          >  2:570778/1‑81 (MQ=255)   gAATGAGCGACGCAGCAGGGCATCAATACCCGGCGCATCAATGGGAATTTCTACTCGAATTAGCATGGTTTAccgaccga                                                                          <  2:570706/80‑1 (MQ=255)   gAATGAGCGACGCAGCAGGGCATCAATACCCGGCGCATCAATGGGAATTTCTACTCGAATTAGCATGGTTCAccgaccga                                                                          <  1:570705/80‑1 (MQ=255)   gAATGAGCGACGCAGCAGGGCATCAATACCCGGCGCATCAATGGGAATTTCTACTCGAATTAGCATGGTTCACCGACCGAt                                                                         >  2:570764/1‑81 (MQ=255)     aTGAGCGACGCAGCAGGGCATCAATACCCGGCGCATCAATGGGAATTTCTACTCGAATTAGCATGGTTCACCGACCGATGt                                                                       >  2:570755/1‑81 (MQ=255)        aGCGACGCAGCAGGGCATCAATACCCGGCGCATCAATGGGAATTTCTACTCGAATTAGCATGGTTCACCGACCGATGTCt                                                                     <  2:570707/80‑1 (MQ=255)          cGACGCAGCAGGGCATCAATACCCGGCGCATCAATGGGAATTTCTACTCGAATTAGCATGGTTCACCGACCGATGTCTGtt                                                                  >  2:570782/1‑81 (MQ=255)               cagcagGGCATCAATACCCGGCGCATCAATGGGAATTTCTACTCGAATTAGCATGGTTCACCGACCGATGTCTGTTTGGtt                                                             <  2:570708/81‑1 (MQ=255)               cagcagGGCATCAATACCCGGCGCATCAATGGGAATTTCTACTCGAATTAGCATGGTTCACCGACCGATGTCTGTTTGGtt                                                             >  1:570756/1‑81 (MQ=255)                 gcagGGCATCAATACCCGGCGCATCAATGGGAATTTCTACTCGAATTAGCATGGTTCACCGACCGATGTCTGTTTGGttt                                                            >  1:570773/1‑80 (MQ=255)                 gcagGGCATCAATACCCGGCGCATCAATGGGAATTTCTACTCGAATTAGCATGGTTCACCGACCGATGTCTGTTTGGTTTc                                                           <  2:570709/81‑1 (MQ=255)                  cagGGCATCAATACCCGGCGCATCAATGGGAATTTCTACTCGAATTAGCATGGTTCACCGACCGATGTCTGTTTGGTTTCa                                                          <  1:570710/81‑1 (MQ=255)                   agGGCATCAATACCCGGCGCATCAATGGGAATTTCTACTCGAATTAGCATGGTTCACCGACCGATGTCTGTTTGGTTTCAg                                                         >  2:570777/1‑81 (MQ=255)                     ggCATCAATACCCGGCGCATCAATGGGAATTTCTACTCGAATTAGCATGGTTCACCGACCGATGTCTGTTTGGTTTCAGgc                                                       <  1:570711/81‑1 (MQ=255)                        aTCAATACCCGGCGCATCAATGGGAATTTCTACTCGAATTAGCATGGTTCACCGACCGATGTCTGTTTGGTTTCAGGcgcg                                                    >  1:570762/1‑81 (MQ=255)                            aTACCCGGCGCATCAATGGGAATTTCTACTCGAATTAGCATGGTTCACCGACCGATGTCTGTTTGGTTTCAGGCGCGGttt                                                <  2:570712/81‑1 (MQ=255)                                 cGGCGCATCAATGGGAATTTCTACTCGAATTAGCATGGTTCACCGACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCAtt                                           <  2:570713/81‑1 (MQ=255)                                  ggCGCATCAATGGGAATTTCTACTCGAATTAGCATGGTTCACCGACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTc                                          >  1:570770/1‑81 (MQ=255)                                   gcgcATCAATGGGAATTTCTACTCGAATTAGCATGGTTCACCGACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTcc                                         <  1:570714/81‑1 (MQ=255)                                      cATCAATGGGAATTTCTACTCGAATTAGCATGGTTCACCGACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGc                                      >  1:570765/1‑81 (MQ=255)                                      cATCAATGGGAATTTCTACTCGAATTAGCATGGTTCACCGACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGc                                      >  1:570772/1‑81 (MQ=255)                                       aTCAATGGGAATTTCTACTCGAATTAGCATGGTTCACCGACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGcc                                     >  1:570781/1‑81 (MQ=255)                                       aTCAATGGGAATTTCTACTCGAATTAGCATGGTTCACCGACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGcc                                     <  1:570715/81‑1 (MQ=255)                                        tCAATGGGAATTTCTACTCGAATTAGCATGGTTCACCGACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGCCt                                    <  1:570716/81‑1 (MQ=255)                                         cAATGGGAATTTCTACTCGAATTAGCATGGTTCACCGACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGCCt                                    >  1:570768/1‑80 (MQ=255)                                          aaTGGGAATTTCTACTCGAATTAGCATGGTTCACCGACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGCCTca                                  <  1:570717/81‑1 (MQ=255)                                          aaTGGGAATTTCTACTCGAATTAGCATGGTTCACCGACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGCCTc                                   <  2:570718/80‑1 (MQ=255)                                           aTGGGAATTTCTACTCGAATTAGCATGGTTCACCGACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGCCTcaa                                 >  1:570771/1‑81 (MQ=255)                                              ggAATTTCTACTCGAATTAGCATGGTTCACCGACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGCCTcaaca                               <  2:570719/80‑1 (MQ=255)                                                aaTTTCTACTCGAATTAGCATGGTTCACCGACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGCCTCAACaaaa                            >  1:570780/1‑81 (MQ=255)                                                        cTCGAATTAGCATGGTTCACCGACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGCCTCAACAAAATCCGcc                      <  1:570721/79‑1 (MQ=255)                                                        cTCGAATTAGCATGGTTCACCGACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGCCTCAACAAAATCCGc                       <  1:570720/78‑1 (MQ=255)                                                           gAATTAGCATGGTTCACCGACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGCCTCAACAAAATCCGCCAGtt                  <  2:570722/80‑1 (MQ=255)                                                             aTTAGCATGGTTCACCGACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGCCTCAACAAAATCCGCCAGTTgca               <  1:570724/81‑1 (MQ=255)                                                             aTTAGCATGGTTCACCGACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGCCTCAACAAAATCCGCCAGTTgca               <  1:570723/81‑1 (MQ=255)                                                                 gCATGGTTCACCGACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGCCTCAACAAAATCCGCCAGTTgcagca            <  1:570725/80‑1 (MQ=255)                                                                  cATGGTTCACCGACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGCCTCAACAAAATCCGCCAGTTgcagcag           >  2:570779/1‑80 (MQ=255)                                                                    tGGTTCACCGACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGcat        <  2:570726/81‑1 (MQ=255)                                                                     ggTTCACCGACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGcat        >  2:570788/1‑80 (MQ=255)                                                                      gTTCACCGACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGcatc       <  1:570729/80‑1 (MQ=255)                                                                      gTTCACCGACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGcatc       <  2:570728/80‑1 (MQ=255)                                                                      gTTCACCGACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATT‑CCGCCTCAACAAAATCCGCCAGTTGCAGCAGcatca      <  2:570727/80‑1 (MQ=255)                                                                        tCACCGACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATg    >  1:570784/1‑81 (MQ=255)                                                                          accgaccgATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATGCg  >  1:570789/1‑81 (MQ=255)                                                                             |                                                                              TCGAATGAGCGACGCAGCAGGGCATCAATACCCGGCGCATCAATGGGAATTTCTACTCGAATTAGCATGGTTCACCTACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATGCG  >  NC_000913/3300691‑3300844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑