Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,308,605	G→T	T107K (ACG→AAG)	nlpI ←	lipoprotein NlpI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,308,605	0	G	T	100.0%	89.4 / NA	24	T107K (ACG→AAG)	nlpI	lipoprotein NlpI
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (9/15);  total (9/15)

TAGTTGTAAGTTGGATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCGAAAAATCGTTACGC  >  NC_000913/3308533‑3308682                                                                         |                                                                              tAGTTGTAAGTTGGATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCTTTAAatat                                                                       <  2:572662/81‑1 (MQ=255)   gTTGTAAGTTGGATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCTTTAAatata                                                                      <  2:572663/80‑1 (MQ=255)    ttGTAAGTTGGATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCTTTAAATATATg                                                                    <  1:572664/81‑1 (MQ=255)       tAAGTTGGATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCTTTAAATATATGCCt                                                                 <  1:572665/81‑1 (MQ=255)          gTTGGATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCTTTAAATATATGCCTAAg                                                              >  2:572694/1‑81 (MQ=255)           ttGGATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCTTTAAATATATGCCTAAGt                                                             <  2:572666/81‑1 (MQ=255)              gATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCTTTAAATATATGCCTAAGTAAt                                                          <  2:572667/81‑1 (MQ=255)               aTCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCTTTAAATATATGCCTAAGTAAtt                                                         <  1:572668/81‑1 (MQ=255)                     cTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCTTTAAATATATGCCTAAGTAATTGAATAc                                                   <  1:572669/81‑1 (MQ=255)                       cAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCTTTAAATATATGCCTAAGTAATTGAATACtt                                                 >  1:572699/1‑81 (MQ=255)                        aaGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCTTTAAATATATGCCTAAGTAATTGAATACt                                                  <  1:572670/79‑1 (MQ=255)                            aCAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCTTTAAATATATGCCTAAGTAATTGAATACTTCAgg                                             >  1:572705/1‑80 (MQ=255)                                    aaaCGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCTTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCgg                                    <  2:572671/81‑1 (MQ=255)                                      aCGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCTTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTc                                  >  2:572696/1‑81 (MQ=255)                                         cTTCATAGGCAGCATCAAAATTGCCTGCCTGCTTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGa                               <  2:572672/81‑1 (MQ=255)                                         cTTCATAGGCAGCATCAAAATTGCCTGCCTGCTTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGa                               >  2:572693/1‑81 (MQ=255)                                          ttCATAGGCAGCATCAAAATTGCCTGCCTGCTTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGAt                              >  2:572692/1‑81 (MQ=255)                                                  cagcaTCAAAATTGCCTGCCTGCTTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAgcg                      >  2:572708/1‑81 (MQ=255)                                                      aTCAAAATTGCCTGCCTGCTTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTg                  >  1:572702/1‑81 (MQ=255)                                                               gcctgcctGCTTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCGAAAAAt          <  1:572673/80‑1 (MQ=255)                                                                    cctgcTTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCGAAAAATCGtt      <  2:572676/79‑1 (MQ=255)                                                                    cctgcTTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCGAAAAATCGtt      <  2:572675/79‑1 (MQ=255)                                                                    cctgcTTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCGAAAAATCGtt      <  2:572674/79‑1 (MQ=255)                                                                      tgcTTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCGAAAAATCGTTAcgc  >  1:572704/1‑81 (MQ=255)                                                                         |                                                                              TAGTTGTAAGTTGGATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCGAAAAATCGTTACGC  >  NC_000913/3308533‑3308682

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑