Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	304,754	C→T	R151Q (CGA→CAA)	ecpE ←	putative fimbrial chaperone EcpE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	304,754	0	C	T	100.0%	127.1 / NA	34	R151Q (CGA→CAA)	ecpE	putative fimbrial chaperone EcpE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/18);  total (16/18)

TAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTT  >  NC_000913/304677‑304825                                                                              |                                                                        tAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGta                                                                      >  1:64022/1‑81 (MQ=255)  aaaCCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGt                                                                       <  1:63968/79‑1 (MQ=255)  aaaCCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGt                                                                       <  2:63969/79‑1 (MQ=255)   aaCCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGtact                                                                    <  1:63970/81‑1 (MQ=255)    gccATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGtacta                                                                   <  2:63971/80‑1 (MQ=255)     ccATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGtactac                                                                  <  2:63972/81‑1 (MQ=255)            ttGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATg                                                           <  1:63973/81‑1 (MQ=255)            ttGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATg                                                           >  1:64016/1‑81 (MQ=255)             tGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATgg                                                          >  1:64015/1‑81 (MQ=255)                     ttacttacCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATc                                                  <  2:63974/81‑1 (MQ=255)                       acttacCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATcac                                                >  2:64018/1‑81 (MQ=255)                       acttacCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATcac                                                >  2:64026/1‑81 (MQ=255)                        cttacCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATcac                                                <  1:63975/80‑1 (MQ=255)                           acCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATcaccac                                             >  2:64023/1‑80 (MQ=255)                            ccGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATcaccacc                                            <  2:63976/80‑1 (MQ=255)                            ccGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATcaccacc                                            <  1:63977/80‑1 (MQ=255)                              gTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCaccaccacc                                         >  2:64019/1‑81 (MQ=255)                                tCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCgg                                       >  2:64032/1‑81 (MQ=255)                                 ccTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCgg                                       <  2:63978/80‑1 (MQ=255)                                    gTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTcc                                   >  2:64024/1‑81 (MQ=255)                                    gTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTcc                                   >  2:64017/1‑81 (MQ=255)                                          ttGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCt                              >  2:64013/1‑80 (MQ=255)                                               gAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATTGTATCCATCACCACCACCGGCTCCGTGCTGACCAc                        <  1:63979/81‑1 (MQ=255)                                                aaGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCAcg                       <  1:63980/81‑1 (MQ=255)                                                aaGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCAcg                       >  1:64021/1‑81 (MQ=255)                                                 aGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCAcgc                      >  2:64020/1‑81 (MQ=255)                                                  ggACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCAcgcc                     <  1:63981/81‑1 (MQ=255)                                                   gACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCAcgccg                    >  1:64025/1‑81 (MQ=255)                                                           aaaCTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTgg            >  1:64030/1‑81 (MQ=255)                                                              cTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCt         <  2:63982/81‑1 (MQ=255)                                                                gAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGc       >  2:64027/1‑81 (MQ=255)                                                                 aaCCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTg        <  2:63983/79‑1 (MQ=255)                                                                 aaCCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGc       <  2:63984/80‑1 (MQ=255)                                                                       aCGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCtt  <  2:63985/79‑1 (MQ=255)                                                                              |                                                                        TAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTT  >  NC_000913/304677‑304825

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑