Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,362,820	C→T	T5M (ACG→ATG)	yhcD →	putative fimbrial usher protein YhcD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,362,820	0	C	T	100.0%	55.2 / NA	16	T5M (ACG→ATG)	yhcD	putative fimbrial usher protein YhcD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/6);  total (10/6)

ATAAATATGTCACTGACTTAAAATAACTTTGCCTGGAGCGACAAGGATGTTAAAAAAAACGTTACTGGCCTACACCATTGGTTTTGCCTTTTCTCCCCCAGCCAATGCAGATGGTATAGAGATTGCCGCTG  >  NC_000913/3362761‑3362891                                                            |                                                                        ataaataTGTCACTGACTTAAAATAACTTTGCCTGGAGCGACAAGGATGTTAAAAAAAATGTTACTGGCCTACACCATTg                                                     <  1:585071/80‑1 (MQ=255)        tGTCACTGACTTAAAATAACTTTGCCTGGAGCGACAAGGATGTTAAAAAAAATGTTACTGGCCTACACCATTGGTTTTGc                                              <  1:585072/80‑1 (MQ=255)               gACTTAAAATAACTTTGCCTGGAGCGACAAGGATGTTAAAAAAAGTGATACTGGCCTACACCAGTGGTTTTGCCTTTtctc                                      >  1:585084/1‑81 (MQ=255)                    aaaaTAACTTTGCCTGGAGCGACAAGGATGTTAAAAAAAATGTTACTGGCCTACACCATTGGTTTTGCCTTTTCTccccc                                  <  2:585073/80‑1 (MQ=255)                        tAACTTTGCCTGGAGCGACAAGGATGTTAAAAAAAATGTTACTGGCCTACACCATTGGTTTTGCCTTTTCTCCCCCAGcc                              >  2:585090/1‑80 (MQ=255)                        tAACTTTGCCTGGAGCGACAAGGATGTTAAAAAAAATGTTACTGGCCTACACCATTGGTTTTGCCTTTTCTCCCCCAGCCa                             <  1:585074/81‑1 (MQ=255)                                ccTGGAGCGACAAGGATGTTAAAAAAAATGTTACTGGCCTACACCATTGGTTTTGCCTTTTCTCCCCCAGCCAATGCAGAt                     <  2:585075/81‑1 (MQ=255)                                  tGGAGCGACAAGGATGTTAAAAAAAATGTTACTGGCCTACACCATTGGTTTTGCCTTTTCTCCCCCAGCCAATGCAGATgg                   >  1:585088/1‑81 (MQ=255)                                     aGCGACAAGGATGTTAAAAAAAATGTTACTGGCCTACACCATTGGTTTTGCCTTTTCTCCCCCAGCCAATGCAGATGGta                 >  1:585086/1‑80 (MQ=255)                                       cGACAAGGATGTTAAAAAAAATGTTACTGGCCTACACCATTGGTTTTGCCTTTTCTCCCCCAGCCAATGCAGATGGta                 >  1:585089/1‑78 (MQ=255)                                       cGACAAGGATGTTAAAAAAAATGTTACTGGCCTACACCATTGGTTTTGCCTTTTCTCCCCCAGCCAATGCAGATGGta                 >  2:585091/1‑78 (MQ=255)                                       cGACAAGGATGTTAAAAAAAATGTTACTGGCCTACACCATTGGTTTTGCCTTTTCTCCCCCAGCCAATGCAGATGGta                 >  2:585093/1‑78 (MQ=255)                                          cAAGGATGTTAAAAAAAATGTTACTGGCCTACACCATTGGTTTTGCCTTTTCTCCCCCAGCCAATGCAGATGGtata               >  2:585095/1‑77 (MQ=255)                                                 gTTAAAAAAAATGTTACTGGCCTACACCATTGGTTTTGCCTTTTCTCCCCCAGCCAATGCAGATGGTATAGAGATTGCCGc    >  1:585083/1‑81 (MQ=255)                                                  ttAAAAAAAATGTTACTGGCCTACACCATTGGTTTTGCCTTTTCTCCCCCAGCCAATGCAGATGGTATAGAGATTGCCGc    <  1:585076/80‑1 (MQ=255)                                                   tAAAAAAAATGTTACTGGCCTACACCATTGGTTTTGCCTTTTCTCCCCCAGCCAATGCAGATGGTATAGAGATTGCCGCTg  >  2:585092/1‑81 (MQ=255)                                                            |                                                                        ATAAATATGTCACTGACTTAAAATAACTTTGCCTGGAGCGACAAGGATGTTAAAAAAAACGTTACTGGCCTACACCATTGGTTTTGCCTTTTCTCCCCCAGCCAATGCAGATGGTATAGAGATTGCCGCTG  >  NC_000913/3362761‑3362891

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑