Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,418,650	A→G	intergenic (+39/‑392)	yhdV → / → yhdW	lipoprotein YhdV/putative ABC transporter periplasmic binding protein YhdW

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,418,650	0	A	G	100.0%	80.2 / NA	23	intergenic (+39/‑392)	yhdV/yhdW	lipoprotein YhdV/putative ABC transporter periplasmic binding protein YhdW
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/13);  total (10/13)

GATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTACATCGCGT  >  NC_000913/3418584‑3418727                                                                   |                                                                              gATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATGCAGTATCTCTCCAt                                                                 <  2:597508/81‑1 (MQ=255)  aTGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATGCAGTATCTCTCCATa                                                                <  1:597509/81‑1 (MQ=255)     gggATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATGCAGTATCTCTCCATAAGa                                                             <  1:597510/81‑1 (MQ=255)            tcGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATGCAGTATCTCTCCATAAGATAAAata                                                      <  1:597511/81‑1 (MQ=255)               ctctATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATGCAGTATCTCTCCATAAGATAAAATATTg                                                   <  2:597512/81‑1 (MQ=255)                    tCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATGCAGTATCTCTCCATAAGATAAAATATTGCtat                                               <  2:597514/80‑1 (MQ=255)                    tCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATGCAGTATCTCTCCATAAGATAAAATATTGCt                                                 <  2:597513/78‑1 (MQ=255)                     cGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATGCAGTATCTCTCCATAAGATAAAATATTGCTatat                                             >  1:597552/1‑81 (MQ=255)                             cTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATGCAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTgg                                     >  2:597550/1‑81 (MQ=255)                               gAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATGCAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTggg                                    >  2:597553/1‑80 (MQ=255)                                aaCAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATGCAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTa                                  <  2:597515/81‑1 (MQ=255)                                      gcgcGGAGTTCCGCGCTCTTTTTAACGATGCAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTccc                            <  2:597516/81‑1 (MQ=255)                                        gcgGAGTTCCGCGCTCTTTTTAACGATGCAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCctg                          >  1:597547/1‑81 (MQ=255)                                              ttCCGCGCTCTTTTTAACGATGCAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGAtt                    >  2:597542/1‑81 (MQ=255)                                                   cgcTCTTTTTAACGATGCAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCa               >  2:597544/1‑81 (MQ=255)                                                    gcTCTTTTTAACGATGCAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCAt              >  2:597545/1‑81 (MQ=255)                                                    gcTCTTTTTAACGATGCAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCAt              >  1:597546/1‑81 (MQ=255)                                                       ctTTTTAACGATGCAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGt            >  2:597551/1‑80 (MQ=255)                                                          tttAACGATGCAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTa          <  2:597517/79‑1 (MQ=255)                                                           ttAACGATGCAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTACAt       >  2:597540/1‑81 (MQ=255)                                                              aCGATGCAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTACATcgc    <  2:597518/81‑1 (MQ=255)                                                              aCGATGCAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTACATcg     <  1:597519/80‑1 (MQ=255)                                                                gATGCAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTACATCGCGt  <  2:597520/81‑1 (MQ=255)                                                                   |                                                                              GATGGGGATCATCGCTCTATCGAAATAACTGAACAGGGCGCGGAGTTCCGCGCTCTTTTTAACGATACAGTATCTCTCCATAAGATAAAATATTGCTATATTCTGCTGGGTAATTCCCTGCTGATTTAGCATGTTACATCGCGT  >  NC_000913/3418584‑3418727

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑