Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,433,348	G→A	N71N (AAC→AAT)	smf ←	protein Smf

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,433,348	0	G	A	100.0%	101.4 / NA	27	N71N (AAC→AAT)	smf	protein Smf
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/11);  total (16/11)

AAACAGTGCGCCGGGGTAATCTGTCGTCGCCAGAAGTTGAGGAGGATAAAATTCGCTGTCCGCAGGAATTAAATGATGGTTGGGTTGCTCCAACCAACAAAGTGAGCTTTCGATACTCTTTCGTGGAAATGAAAGAAAGCGTTGTGCCTGCCG  >  NC_000913/3433270‑3433422                                                                               |                                                                           aaacaGTGCGCCGGGGTAATCTGTCGTCGCCAGAAGTTGAGGAGGATAAAATTCGCTGTCCGCAGGAATTAAATGATgatt                                                                          >  2:600656/1‑81 (MQ=255)  aacaGTGCGCCGGGGTAATCTGTCGTCGCCAGAAGTTGAGGAGGATAAAATTCGCTGTCCGCAGGAATTAAATGATgattg                                                                         <  1:600629/81‑1 (MQ=255)   acaGTGCGCCGGGGTAATCTGTCGTCGCCAGAAGTTGAGGAGGATAAAATTCGCTGTCCGCAGGAATTAAATGATgattgg                                                                        <  1:600630/81‑1 (MQ=255)         cgcCGGGGTAATCTGTCGTCGCCAGAAGTTGAGGAGGATAAAATTCGCTGTCCGCAGGAATTAAATGATGATTGGGTTGCt                                                                  <  2:600631/81‑1 (MQ=255)           ccGGGGTAATCTGTCGTCGCCAGAAGTTGAGGAGGATAAAATTCGCTGTCCGCAGGAATTAAATGATGATTGGGTTGCTcc                                                                <  1:600632/81‑1 (MQ=255)                   aTCTGTCGTCGCCAGAAGTTGAGGAGGATAAAATTCGCTGTCCGCAGGAATTAAATGATGATTGGGTTGCTCcaaccaac                                                         >  2:600659/1‑80 (MQ=255)                   aTCTGTCGTCGCCAGAAGTTGAGGAGGATAAAATTCGCTGTCCGCAGGAATTAAATGATGATTGGGTTGCTCcaaccaac                                                         >  1:600671/1‑80 (MQ=255)                        tcgtcgCCAGAAGTTGAGGAGGATAAAATTCGCTGTCCGCAGGAATTAAATGATGATTGGGTTGCTCCAACCAACAAAGt                                                    <  2:600633/80‑1 (MQ=255)                             gCCAGAAGTTGAGGAGGATAAAATTCGCTGTCCGCAGGAATTAAATGATGATTGGGTTGCTCCAACCAACAAAGTGAGCtt                                              <  2:600634/81‑1 (MQ=255)                              ccAGAAGTTGAGGAGGATAAAATTCGCTGTCCGCAGGAATTAAATGATGATTGGGTTGCTCCAACCAACAAAGTGAGCttt                                             >  2:600677/1‑81 (MQ=255)                               cAGAAGTTGAGGAGGATAAAATTCGCTGTCCGCAGGAATTAAATGATGATTGGGTTGCTCCAACCAACAAAGTGAGCTTTc                                            >  1:600660/1‑81 (MQ=255)                                aGAAGTTGAGGAGGATAAAATTCGCTGTCCGCAGGAATTAAATGATGATTGGGTTGCTCCAACCAACAAAGTGAGCTTTc                                            >  1:600676/1‑80 (MQ=255)                                   aGTTGAGGAGGATAAAATTCGCTGTCCGCAGGAATTAAATGATGATTGGGTTGCTCCAACCAACAAAGTGAGCTTTCGATa                                        <  2:600635/81‑1 (MQ=255)                                       gaggagGATAAAATTCGCTGTCCGCAGGAATTAAATGATGATTGGGTTGCTCCAACCAACAAAGTGAGCTTTCGATActct                                    >  1:600668/1‑81 (MQ=255)                                       gaggagGATAAAATTCGCTGTCCGCAGGAATTAAATGATGATTGGGTTGCTCCAACCAACAAAGTGAGCTTTCGATActc                                     <  1:600636/80‑1 (MQ=255)                                           aggaTAAAATTCGCTGTCCGCAGGAATTAAATGATGATTGGGTTGCTCCAACCAACAAAGTGAGCTTTCGATACTCTTTCg                                >  1:600667/1‑81 (MQ=255)                                              aTAAAATTCGCTGTCCGCAGGAATTAAATGATGATTGGGTTGCTCCAACCAACAAAGTGAGCTTTCGATACTCTTTCGTg                              >  1:600672/1‑80 (MQ=255)                                               tAAAATTCGCTGTCCGCAGGAATTAAATGATGATTGGGTTGCTCCAACCAACAAAGTGAGCTTTCGATACTCTTTCGTgg                             >  1:600675/1‑80 (MQ=255)                                                 aaaTTCGCTGTCCGCAGGAATTAAATGATGATTGGGTTGCTCCAACCAACAAAGTGAGCTTTCGATACTCTTTCGTGGaaa                          <  2:600637/81‑1 (MQ=255)                                                  aaTTCGCTGTCCGCAGGAATTAAATGATGATTGGGTTGCTCCAACCAACAAAGTGAGCTTTCGATACTCTTTCGTGGAAAt                         >  1:600680/1‑81 (MQ=255)                                                   aTTCGCTGTCCGCAGGAATTAAATGATGATTGGGTTGCTCCAACCAACAAAGTGAGCTTTCGATACTCTTTCGTGGAAAt                         >  2:600663/1‑80 (MQ=255)                                                        cTGTCCGCAGGAATTAAATGATGATTGGGTTGCTCCAACCAACAAAGTGAGCTTTCGATACTCTTTCGTGGAAATgaaag                    >  1:600661/1‑80 (MQ=255)                                                          gTCCGCAGGAATTAAATGATGATTGGGTTGCTCCAACCAACAAAGTGAGCTTTCGATACTCTTTCGTGGAAATgaaaga                   >  2:600673/1‑79 (MQ=255)                                                          gTCCGCAGGAATTAAATGATGATTGGGTTGCTCCAACCAACAAAGTGAGCTTTCGATACTCTTTCGTGGAAATgaaaga                   >  2:600681/1‑79 (MQ=255)                                                           tCCGCAGGAATTAAATGATGATTGGGTTGCTCCAACCAACAAAGTGAGCTTTCGATACTCTTTCGTGGAAATgaaagaa                  <  2:600638/79‑1 (MQ=255)                                                            ccGCAGGAATTAAATGATGATTGGGTTGCTCCAACCAACAAAGTGAGCTTTCGATACTCTTTCGTGGAAATGAAAGAAAGc               >  1:600679/1‑81 (MQ=255)                                                             cGCAGGAATTAAATGATGATTGGGTTGCTCCAACCAACAAAGTGAGCTTTCGATACTCTTTCGTGGAAATGAAAGAAAGc               <  2:600639/80‑1 (MQ=255)                                                                        aaTGATGATTGGGTTGCTCCAACCAACAAAGTGAGCTTTCGATACTCTTTCGTGGAAATGAAAGAAAGCGTTGtgcctgcc   <  2:600640/81‑1 (MQ=255)                                                                        aaTGATGATTGGGTTGCTCCAACCAACAAAGTGAGCTTTCGATACTCTTTCGTGGAAATGAAAGAAAGCGTTGtgcctgcc   <  1:600641/81‑1 (MQ=255)                                                                         atgatgATTGGGTTGCTCCAACCAACAAAGTGAGCTTTCGATACTCTTTCGTGGAAATGAAAGAAAGCGTTGTGCCTGCCg  >  1:600691/1‑81 (MQ=255)                                                                               |                                                                           AAACAGTGCGCCGGGGTAATCTGTCGTCGCCAGAAGTTGAGGAGGATAAAATTCGCTGTCCGCAGGAATTAAATGATGGTTGGGTTGCTCCAACCAACAAAGTGAGCTTTCGATACTCTTTCGTGGAAATGAAAGAAAGCGTTGTGCCTGCCG  >  NC_000913/3433270‑3433422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑