Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,447,858	G→T	R98S (CGT→AGT)	rplN ←	50S ribosomal subunit protein L14

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,447,858	0	G	T	100.0%	113.1 / NA	30	R98S (CGT→AGT)	rplN	50S ribosomal subunit protein L14
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (14/16);  total (14/16)

TCTGGTGCCAGAGAGATAATTTTCATGAACTTCTCACTACGAAGCTCACGAGTTACCGGCCCAAAAATACGCGTACCGATAGGCTGCTCGCTGTTGTTGTTCAGAAGAACACAAGCATTACCATCGAAGCGAATGACAGAACCGTC  >  NC_000913/3447788‑3447933                                                                       |                                                                            tCTGGTGCCAGAGAGATAATTTTCATGAACTTCTCACTACGAAGCTCACGAGTTACCGGCCCAAAAATACTCGTACCGa                                                                     >  1:603865/1‑79 (MQ=255)  cTGGTGCCAGAGAGATAATTTTCATGAACTTCTCACTACGAAGCTCACGAGTTACCGGCCCAAAAATACTCGTACCGATa                                                                   <  2:603820/80‑1 (MQ=255)     gTGCCAGAGAGATAATTTTCATGAACTTCTCACTACGAAGCTCACGAGTTACCGGCCCAAAAATACTCGTACCGATAGgct                                                               >  1:603852/1‑81 (MQ=255)         cAGAGAGATAATTTTCATGAACTTCTCACTACGAAGCTCACGAGTTACCGGCCCAAAAATACTCGTACCGATAGGCTGCTc                                                           <  2:603821/81‑1 (MQ=255)         cAGAGAGATAATTTTCATGAACTTCTCACTACGAAGCTCACGAGTTACCGGCCCAAAAATACTCGTACCGATAGGCTGCTc                                                           >  1:603848/1‑81 (MQ=255)            agagaTAATTTTCATGAACTTCTCACTACGAAGCTCACGAGTTACCGGCCCAAAAATACTCGTACCGATAGGCTGCTCGCt                                                        <  2:603823/81‑1 (MQ=255)            agagaTAATTTTCATGAACTTCTCACTACGAAGCTCACGAGTTACCGGCCCAAAAATACTCGTACCGATAGGCTGCTCGCt                                                        <  2:603822/81‑1 (MQ=255)                 tAATTTTCATGAACTTCTCACTACGAAGCTCACGAGTTACCGGCCCAAAAATACTCGTACCGATAGGCTGCTCGCtgttgt                                                   <  1:603825/81‑1 (MQ=255)                 tAATTTTCATGAACTTCTCACTACGAAGCTCACGAGTTACCGGCCCAAAAATACTCGTACCGATAGGCTGCTCGCtgttgt                                                   <  1:603824/81‑1 (MQ=255)                       tCATGAACTTCTCACTACGAAGCTCACGAGTTACCGGCCCAAAAATACTCGTACCGATAGGCTGCTCGCTgttgttgtt                                               >  1:603877/1‑79 (MQ=255)                       tCATGAACTTCTCACTACGAAGCTCACGAGTTACCGGCCCAAAAATACTCGTACCGATAGGCTGCTCGCTGTTGTTGTTc                                              <  2:603826/80‑1 (MQ=255)                        cATGAACTTCTCACTACGAAGCTCACGAGTTACCGGCCCAAAAATACTCGTACCGATAGGCTGCTCGCTgttgttgtt                                               >  2:603851/1‑78 (MQ=255)                        cATGAACTTCTCACTACGAAGCTCACGAGTTACCGGCCCAAAAATACTCGTACCGATAGGCTGCTCGCTGTTGTTGTTCa                                             <  1:603827/80‑1 (MQ=255)                         aTGAACTTCTCACTACGAAGCTCACGAGTTACCGGCCCAAAAATACTCGTACCGATAGGCTGCTCGCTGTTGTTGTTCaga                                           <  2:603828/81‑1 (MQ=255)                             aCTTCTCACTACGAAGCTCACGAGTTACCGGCCCAAAAATACTCGTACCGATAGGCTGCTCGCTGTTGTTGTTCAgaagaa                                       <  2:603829/81‑1 (MQ=255)                                    aCTACGAAGCTCACGAGTTACCGGCCCAAAAATACTCGTACCGATAGGCTGCTCGCTGTTGTTGTTCAGAAGAACACAAg                                 >  2:603871/1‑80 (MQ=255)                                    aCTACGAAGCTCACGAGTTACCGGCCCAAAAATACTCGTACCGATAGGCTGCTCGCTGTTGTTGTTCAGAAGAACACAAg                                 >  2:603870/1‑80 (MQ=255)                                          aaGCTCACGAGTTACCGGCCCAAAAATACTCGTACCGATAGGCTGCTCGCTGTTGTTGTTCAGAAGAACACAAGCATTAcc                          <  1:603830/81‑1 (MQ=255)                                             cTCACGAGTTACCGGCCCAAAAATACTCGTACCGATAGGCTGCTCGCTGTTGTTGTTCAGAAGAACACAAGCATTACCATc                       >  1:603863/1‑81 (MQ=255)                                               cACGAGTTACCGGCCCAAAAATACTCGTACCGATAGGCTGCTCGCTGTTGTTGTTCAGAAGAACACAAGCATTACCATCg                      >  2:603868/1‑80 (MQ=255)                                                aCGAGTTACCGGCCCAAAAATACTCGTACCGATAGGCTGCTCGCTGTTGTTGTTCAGAAGAACACAAGCATTACCATCg                      >  2:603854/1‑79 (MQ=255)                                                    gTTACCGGCCCAAAAATACTCGTACCGATAGGCTGCTCGCTGTTGTTGTTCAGAAGAACACAAGCATTACCATCGAAGCGa                <  2:603831/81‑1 (MQ=255)                                                     ttACCGGCCCAAAAATACTCGTACCGATAGGCTGCTCGCTGTTGTTGTTCAGAAGAACACAAGCATTACCATCGAAGCGaa               <  2:603832/81‑1 (MQ=255)                                                       aCCGGCCCAAAAATACTCGTACCGATAGGCTGCTCGCTGTTGTTGTTCAGAAGAACACAAGCATTACCATCGAAGCGAAt              <  1:603833/80‑1 (MQ=255)                                                       aCCGGCCCAAAAATACTCGTACCGATAGGCTGCTCGCTGTTGTTGTTCAGAAGAACACAAGCATTACCATCGAAGCGAAt              <  1:603834/80‑1 (MQ=255)                                                       aCCGGCCCAAAAATACTCGTACCGATAGGCTGCTCGCTGTTGTTGTTCAGAAGAACACAAGCATTACCATCGAAGCGAAt              >  1:603859/1‑80 (MQ=255)                                                        ccGGCCCAAAAATACTCGTACCGATAGGCTGCTCGCTGTTGTTGTTCAGAAGAACACAAGCATTACCATCGAAGCGAATGa            <  2:603835/81‑1 (MQ=255)                                                              cAAAAATACTCGTACCGATAGGCTGCTC‑‑‑GATGTTGTTCAGAAGAACACAAGCATTACCATCGAAGCGAATGACAGAACCGt   >  2:603855/1‑81 (MQ=255)                                                                  aaTACTCGTACCGATAGGCTGCTCGCTGTTGTTGTTCAGAAGAACACAAGCATTACCATCGAAGCGAATGACAGAACCGTc  >  2:603862/1‑81 (MQ=255)                                                                  aaTACTCGTACCGATAGGCTGCTCGCTGTTGTTGTTCAGAAGAACACAAGCATTACCATCGAAGCGAATGACAGAACCGTc  >  2:603864/1‑81 (MQ=255)                                                                       |                                                                            TCTGGTGCCAGAGAGATAATTTTCATGAACTTCTCACTACGAAGCTCACGAGTTACCGGCCCAAAAATACGCGTACCGATAGGCTGCTCGCTGTTGTTGTTCAGAAGAACACAAGCATTACCATCGAAGCGAATGACAGAACCGTC  >  NC_000913/3447788‑3447933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑