Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,471,700	A→T	F605L (TTT→TTA)	fusA ←	elongation factor G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,471,700	0	A	T	100.0%	73.6 / NA	20	F605L (TTT→TTA)	fusA	elongation factor G
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (13/7);  total (13/7)

ACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCGCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAAC  >  NC_000913/3471623‑3471773                                                                              |                                                                          aCGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCGCTTTCTTtaa                                                                         <  1:608959/80‑1 (MQ=255)  cGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCGCTTTCTTTAAGc                                                                       >  2:608987/1‑81 (MQ=255)        cGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCGCTTTCTTTAAGCcttctt                                                                 <  1:608960/81‑1 (MQ=255)         ggTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCGCTTTCTTTAAGCCTTCttt                                                                <  2:608961/81‑1 (MQ=255)              tctcTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCGCTTTCTTTAAGCCTTCTTTAAAgg                                                           >  2:608997/1‑81 (MQ=255)                     cGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCGCTTTCTTTAAGCCTTCTTTAAAGGCGATAGa                                                    <  1:608962/81‑1 (MQ=255)                              tACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCGCTTTCTTTAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAg                                           >  2:608989/1‑81 (MQ=255)                                   cAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCGCTTTCTTTAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTa                                       >  2:608990/1‑80 (MQ=255)                                    aaCCTTCATGATCGGCTCAAGCAGAACTGGTTTCGCTTTCTTTAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTaaa                                     >  1:608999/1‑81 (MQ=255)                                           aTGATCGGCTCAAGCAGAACTGGTTTCGCTTTCTTTAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGt                              >  1:608996/1‑81 (MQ=255)                                            tGATCGGCTCAAGCAGAACTGGTTTCGCTTTCTTTAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGtt                             <  1:608963/81‑1 (MQ=255)                                            tGATCGGCTCAAGCAGAACTGGTTTCGCTTTCTTTAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGtt                             >  2:608993/1‑81 (MQ=255)                                             gATCGGCTCAAGCAGAACTGGTTTCGCTTTCTTTAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGtt                             >  2:608991/1‑80 (MQ=255)                                                 ggCTCAAGCAGAACTGGTTTCGCTTTCTTTAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCagag                        >  2:609003/1‑81 (MQ=255)                                                       aGCAGAACTGGTTTCGCTTTCTTTAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCAGAGGAGTCa                  <  1:608964/81‑1 (MQ=255)                                                          aGAACTGGTTTCGCTTTCTTTAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCAGAGGAGTCAACg               >  2:608998/1‑81 (MQ=255)                                                            aaCTGGTTTCGCTTTCTTTAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCAGAGGAGTCAACGt              >  2:609004/1‑80 (MQ=255)                                                                 gTTTCGCTTTCTTTAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCAGAGGAGTCAACGTCATgg         <  2:608966/80‑1 (MQ=255)                                                                 gTTTCGCTTTCTTTAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCAGAGGAGTCAACGTCATgg         <  2:608965/80‑1 (MQ=255)                                                                   ttCGCTTTCTTTAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCAGAGGAGTCAACGTCATGGt        >  1:609008/1‑79 (MQ=255)                                                                      gCTTTCTTTAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGaa   >  1:609016/1‑81 (MQ=255)                                                                       ctttctttAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAAc  >  1:609002/1‑81 (MQ=255)                                                                              |                                                                          ACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCGCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCAGAGGAGTCAACGTCATGGTAAGAAC  >  NC_000913/3471623‑3471773

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑