Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,795,757	G→C	A277P (GCG→CCG)	waaF →	ADP‑heptose‑‑LPS heptosyltransferase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,795,757	0	G	C	100.0%	83.3 / NA	24	A277P (GCG→CCG)	waaF	ADP‑heptose‑‑LPS heptosyltransferase 2
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (15/9);  total (15/9)

GCTTGATCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGGCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTATCCCAT  >  NC_000913/3795681‑3795828                                                                             |                                                                        gCTTGATCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTgcgccggc                                                                     <  2:685821/81‑1 (MQ=255)        cAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAAt                                                              >  2:685868/1‑81 (MQ=255)         aaGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATc                                                             >  2:685853/1‑81 (MQ=255)         aaGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATc                                                             >  1:685867/1‑81 (MQ=255)         aaGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATc                                                             <  2:685822/81‑1 (MQ=255)                 aTCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTg                                                     >  1:685863/1‑81 (MQ=255)                      gATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGc                                                >  2:685861/1‑81 (MQ=255)                      gATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGc                                                >  2:685875/1‑81 (MQ=255)                                  tAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGt                                      <  2:685824/79‑1 (MQ=255)                                  tAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGt                                      <  1:685823/79‑1 (MQ=255)                                    aaGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTcc                                    >  1:685854/1‑79 (MQ=255)                                      gCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAg                                 >  2:685871/1‑80 (MQ=255)                                      gCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGt                                <  1:685825/81‑1 (MQ=255)                                       ccATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAg                                 >  1:685877/1‑79 (MQ=255)                                       ccATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTa                               <  1:685826/81‑1 (MQ=255)                                          ttGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGcc                            <  1:685827/81‑1 (MQ=255)                                           tGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGccc                           >  1:685872/1‑81 (MQ=255)                                              cACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGa                        >  1:685860/1‑81 (MQ=255)                                                atAAAGATTATGGAATGATGAATGTTGAGCAGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACt                      <  1:889531/80‑1 (MQ=255)                                                  aaCGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACtt                     >  2:685857/1‑80 (MQ=255)                                                   aCGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTca                   >  2:685865/1‑81 (MQ=255)                                                    cGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTca                   >  2:685884/1‑80 (MQ=255)                                                            gCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACAccgcc            <  1:685828/79‑1 (MQ=255)                                                                    cATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTATCCCAt  <  2:685829/81‑1 (MQ=255)                                                                     aTGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTATCCCAt  >  1:685876/1‑80 (MQ=255)                                                                             |                                                                        GCTTGATCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGGCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTATCCCAT  >  NC_000913/3795681‑3795828

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑