Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,839,590	G→A	A135V (GCG→GTG)	nlpA ←	lipoprotein‑28

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,839,590	0	G	A	100.0%	112.4 / NA	30	A135V (GCG→GTG)	nlpA	lipoprotein‑28
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/9);  total (21/9)

CCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTGGAATAACCCGCCATAGGGAAGACAAA  >  NC_000913/3839513‑3839663                                                                              |                                                                          ccTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCAcc                                                                         <  1:695148/80‑1 (MQ=255)  ctctTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATcaccac                                                                       <  1:695149/81‑1 (MQ=255)    ctTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCACCACTg                                                                     >  2:695167/1‑81 (MQ=255)        cAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCACCACTGTcgc                                                                 >  2:695172/1‑81 (MQ=255)        cAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCACCACTGTcgc                                                                 >  1:695173/1‑81 (MQ=255)         agagTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCACCACTGTcgcg                                                                >  2:695185/1‑81 (MQ=255)                  aGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCACCACTGTCGCGCCTTCttt                                                        >  1:695187/1‑80 (MQ=255)                    cTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCACCACTGTCGCGCCTTCTTTTAt                                                     <  2:695150/81‑1 (MQ=255)                        tctTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCACCACTGTCGCGCCTTCTTTTATTTgc                                                 >  2:695175/1‑81 (MQ=255)                         ctTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCACCACTGTCGCGCCTTCTTTTATTTgcg                                                >  2:695264/1‑81 (MQ=255)                         ctTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCACCACTGTCGCGCCTTCTTTTATTTgcg                                                >  1:695184/1‑81 (MQ=255)                            tttGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCACCACTGTCGCGCCTTCTTTTATTTgcgc                                               <  1:695151/79‑1 (MQ=255)                               gCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCACCACTGTCGCGCCTTCTTTTATTTGCGCCACgg                                          >  1:695181/1‑81 (MQ=255)                                cAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCACCACTGTCGCGCCTTCTTTTATTTGCGCCACgg                                          <  1:695152/80‑1 (MQ=255)                                    aGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCACCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTg                                     <  2:695153/81‑1 (MQ=255)                                     gTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCACCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGa                                    >  1:695188/1‑81 (MQ=255)                                       aaaaGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCACCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGAtt                                  >  1:695197/1‑81 (MQ=255)                                        aaaGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCACCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGAttt                                 >  1:695170/1‑81 (MQ=255)                                         aaGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCACCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGAtttt                                >  2:695178/1‑81 (MQ=255)                                             gcgcGACCAAGGTTGGTCGGGTCGTTGGGGATCACCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTg                            >  2:695190/1‑81 (MQ=255)                                              cgcgACCAAGGTTGGTCGGGTCGTTGGGGATCACCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTg                            <  1:695154/80‑1 (MQ=255)                                                cgACCAAGGTTGGTCGGGTCGTTGGGGATCACCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTGGaa                         >  1:695177/1‑81 (MQ=255)                                                 gACCAAGGTTGGTCGGGTCGTTGGGGATCACCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTGGaa                         >  1:695194/1‑80 (MQ=255)                                                 gACCAAGGTTGGTCGGGTCGTTGGGGATCACCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTGGAAt                        <  2:695155/81‑1 (MQ=255)                                                    cAAGGTTGGTCGGGTCGTTGGGGATCACCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTGGAATAAc                     >  2:695193/1‑81 (MQ=255)                                                    cAAGGTTGGTCGGGTCGTTGGGGATCACCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTGGAATAAc                     >  1:695179/1‑81 (MQ=255)                                                          tGGTCGGGTCGTTGGGGATCACCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTGGAATAACCCGCCa               >  2:695203/1‑81 (MQ=255)                                                               gggtcgTTGGGGATCACCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTGGAATAACCCGCCATAggg          <  2:695156/81‑1 (MQ=255)                                                               gggtcgTTGGGGATCACCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTGGAATAACCCGCCATAgg           <  2:695157/80‑1 (MQ=255)                                                                    gTTGGGGATCACCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTGGAATAACCCGCCATAGGGAAGa      >  2:695180/1‑80 (MQ=255)                                                                       ggggATCACCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTGGAATAACCCGCCATAGGGAAGACaaa  >  1:695186/1‑81 (MQ=255)                                                                              |                                                                          CCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTGGAATAACCCGCCATAGGGAAGACAAA  >  NC_000913/3839513‑3839663

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑