| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,852,657 | T→C | Y43C (TAC→TGC) | ilvB ← | acetohydroxy acid synthase I subunit IlvB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,852,657 | 0 | T | C | 97.6% | 145.6 / ‑3.7 | 42 | Y43C (TAC→TGC) | ilvB | acetohydroxy acid synthase I subunit IlvB |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (9/32); total (9/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-01 | |||||||||||
CTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGTAAACAGGCAGGATAGAACCGCCCGGAATGCCTGTCACAATCTTAATGCCCTGCTGTTCCAGGAAATGAACGATAAAT > NC_000913/3852581‑3852734 | cTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGCAAAc < 1:698516/81‑1 (MQ=255) aGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGCAAAcagg < 1:698517/81‑1 (MQ=255) aGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGCAAAcag < 2:698518/80‑1 (MQ=255) gATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGCAAAcaggcag > 1:698585/1‑81 (MQ=255) tAAAGCCCGCGCCCTGTTCATGACGGGCCAGGATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGCAAACAGGCAGGa < 2:698519/81‑1 (MQ=255) tAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGCAAACAGGCAGGa < 1:698520/81‑1 (MQ=255) aaGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGCAAACAGGCAGGAt < 1:698521/80‑1 (MQ=255) aaGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCCTAAGGCATCGCAAACAGGCAGGATa < 1:698522/81‑1 (MQ=255) aGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGCAAACAGGCAGGATAg < 2:698523/81‑1 (MQ=255) ccGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGCAAACAGGCAGGATAGAAc < 2:698524/81‑1 (MQ=255) cgcCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGCAAACAGGCAGGATAGAACCGc > 2:698587/1‑81 (MQ=255) gcCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGCAAACAGGCAGGATAGAACCGcc < 2:698525/81‑1 (MQ=255) cccTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGCAAACAGGCAGGATAGAACCGccc < 2:698527/81‑1 (MQ=255) cccTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGCAAACAGGCAGGATAGAACCGccc < 2:698526/81‑1 (MQ=255) cTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGCAAACAGGCAGGATAGAACCGCCCgg < 1:698528/81‑1 (MQ=255) ttCATGGCGCGCCAGAATATGACGGATTTGCGTGCTTTGGCTTAACGCGTCGTAAACGGGGAGGATCGAACCACCGGGAAt < 2:892674/81‑1 (MQ=255) ttCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGCAAACAGGCAGGATAGAACCGCCCGGa < 2:698530/79‑1 (MQ=255) ttCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGCAAACAGGCAGGATAGAACCGCCCGGAAt < 2:698529/81‑1 (MQ=255) tCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGCAAACAGGCAGGATAGAACCGCCCGGAATg > 1:698594/1‑81 (MQ=255) cATGACGGGCCAGAATATGGCGGAGTTGCGTGCTTTGGCTTAAGGCATCGCAAACAGGCAGGATAGAACCGCCCGGAATg < 1:698531/80‑1 (MQ=255) aTGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGCAAACAGGCAGGATAGAACCGCCCGGAATGc < 1:698532/80‑1 (MQ=255) tGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGCAAACAGGCAGGATAGAACCGCCCGGAATGCCt > 2:698602/1‑81 (MQ=255) aCGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGCAAACAGGCAGGATAGAACCGCCCGGAATGCCTGt < 1:698533/81‑1 (MQ=255) atatGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGCAAACAGGCAGGATAGAACCGCCCGGAATGCCTGTCACAATCTTa < 2:698534/81‑1 (MQ=255) tatGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGCAAACAGGCAGGATAGAACCGCCCGGAATGCCTGTCACAATCTTaa < 1:698535/81‑1 (MQ=255) gCGGATTTGCGTGCTTTGGCTTAAGGCATCGCAAACAGGCAGGATAGAACCGCCCGGAATGCCTGTCACAATCTTAATGcc > 1:698603/1‑81 (MQ=255) gCGGATTTGCGTGCTTTGGCTTAAGGCATCGCAAACAGGCAGGATAGAACCGCCCGGAATGCCTGTCACAATCTTAATGc < 1:698536/80‑1 (MQ=255) cGGATTTGCGTGCTTTGGCTTAAGGCATCGCAAACAGGCAGGATAGAACCGCCCGGAATGCCTGTCACAATCTTAATGccc < 1:698537/81‑1 (MQ=255) cGGATTTGCGTGCTTTGGCTTAAGGCATCGCAAACAGGCAGGATAGAACCGCCCGGAATGCCTGTCACAATCTTAATGccc > 1:698606/1‑81 (MQ=255) ggATTTGCGTGCTTTGGCTTAAGGCATCGCAAACAGGCAGGATAGAACCGCCCGGAATGCCTGTCACAATCTTAATGCCct < 1:698538/81‑1 (MQ=255) cGTGCTTTGGCTTAAGGCATCGCAAACAGGCAGGATAGAACCGCCCGGAATGCCTGTCACAATCTTAATGCCCTGCTGTTc < 1:698539/81‑1 (MQ=255) gCTTTGGCTTAAGGCATCGCAAACAGGCAGGATAGAACCGCCCGGAATGCCTGTCACAATCTTAATGCCCTGCTGTTCCAg > 2:698598/1‑81 (MQ=255) gCTTTGGCTTAAGGCATCGCAAACAGGCAGGATAGAACCGCCCGGAATGCCTGTCACAATCTTAATGCCCTGCTGTTCCAg > 2:698600/1‑81 (MQ=255) cTTTGGCTTAAGGCATCGCAAACAGGCAGGATAGAACCGCCCGGAATGCCTGTCACAATCTTAATGCCCTGCTGTTCCAgg < 2:698540/81‑1 (MQ=255) cTTTGGCTTAAGGCATCGCAAACAGGCAGGATAGAACCGCCCGGAATGCCTGTCACAATCTTAATGCCCTGCTGTTCCAgg < 2:698541/81‑1 (MQ=255) tGGCTTAAGGCATCGCAAACAGGCAGGATAGAACCGCCCGGAATGCCTGTCACAATCTTAATGCCCTGCTGTTCCAGGa < 2:698542/79‑1 (MQ=255) tGGCTTAAGGCATCGCAAACAGGCAGGATAGAACCGCCCGGAATGCCTGTCACAATCTTAATGCCCTGCTGTTCCAGGa < 2:698543/79‑1 (MQ=255) ggCTTAAGGCATCGCAAACAGGCAGGATAGAACCGCCCGGAATGCCTGTCACAATCTTAATGCCCTGCTGTTCCAGGAAAt > 1:698590/1‑81 (MQ=255) gCTTAAGGCATCGCAAACAGGCAGGATAGAACCGCCCGGAATGCCTGTCACAATCTTAATGCCCTGCTGTTCCAGGAAAt < 1:698544/80‑1 (MQ=255) aaGGCATCGCAAACAGGCAGGATAGAACCGCCCGGAATGCCTGTCACAATCTTAATGCCCTGCTGTTCCAGGAAATGAAc < 1:698545/80‑1 (MQ=255) cATCGCAAACAGGCAGGATAGAACCGCCCGGAATGCCTGTCACAATCTTAATGCCCTGCTGTTCCAGGAAATGAACGATaa < 1:698546/81‑1 (MQ=255) tCGCAAACAGGCAGGATAGAACCGCCCGGAATGCCTGTCACAATCTTAATGCCCTGCTGTTCCAGGAAATGAACGATAAAt < 1:698547/81‑1 (MQ=255) | CTGAGCGATAAAGCCCGCGCCCTGTTCATGACGGGCCAGAATATGGCGGATTTGCGTGCTTTGGCTTAAGGCATCGTAAACAGGCAGGATAGAACCGCCCGGAATGCCTGTCACAATCTTAATGCCCTGCTGTTCCAGGAAATGAACGATAAAT > NC_000913/3852581‑3852734 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |