Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,863,817	A→T	intergenic (‑214/‑82)	glvC ← / → yidP	putative PTS enzyme II component GlvC/putative DNA‑binding transcriptional regulator YidP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,863,817	0	A	T	100.0%	84.5 / NA	23	intergenic (‑214/‑82)	glvC/yidP	putative PTS enzyme II component GlvC/putative DNA‑binding transcriptional regulator YidP
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (9/14);  total (9/14)

TGGGCGGTAGAGATCTACGTTTTCGCGAAAAAGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTAAAAAAAAGATGTTTTTTCAATCGATTAAGCAGAACTTGTGTGCGCATTACCCGGGCTTGCAGGCAAAAAAGAGATC  >  NC_000913/3863741‑3863893                                                                             |                                                                             tGGGCGGTAGAGATCTACGTTTTCGCGAAAAAGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTtaaa                                                                           >  2:701335/1‑80 (MQ=255)  gggCGGTAGAGATCTACGTTTTCGCGAAAAAGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTtaaaa                                                                          <  1:701288/80‑1 (MQ=255)   ggCGGTAGAGATCTACGTTTTCGCGAAAAAGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTtaaaaaa                                                                        <  1:701289/81‑1 (MQ=255)     cGGTAGAGATCTACGTTTTCGCGAAAAAGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAg                                                                      <  1:701290/81‑1 (MQ=255)            gaTCTACGTTTTCGCGAAAAAGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGtttt                                                               <  2:701291/81‑1 (MQ=255)                  cGTTTTCGCGAAAAAGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCaa                                                          <  2:701296/80‑1 (MQ=255)                  cGTTTTCGCGAAAAAGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCa                                                           <  1:701292/79‑1 (MQ=255)                  cGTTTTCGCGAAAAAGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCa                                                           <  1:701295/79‑1 (MQ=255)                  cGTTTTCGCGAAAAAGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCa                                                           <  2:701294/79‑1 (MQ=255)                  cGTTTTCGCGAAAAAGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCa                                                           <  1:701293/79‑1 (MQ=255)                       tCGCGAAAAAGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATCGAt                                                     <  2:701297/80‑1 (MQ=255)                               aaGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATCGATTAAGCAGaa                                            >  2:701331/1‑81 (MQ=255)                                   aGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATCGATTAAGCAGAACTtg                                        >  2:701327/1‑81 (MQ=255)                                     aCCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATCGATTAAGCAGAACTtgtg                                      >  2:701321/1‑81 (MQ=255)                                            tgAGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATCGATTAAGCAGAACTTGTGTgcgc                                 <  1:701298/79‑1 (MQ=255)                                             gAGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATCGATTAAGCAGAACTTGTGTGCGCAt                               >  2:701334/1‑80 (MQ=255)                                              aGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATCGATTAAGCAGAACTTGTGTGCGCATTa                             >  1:701340/1‑81 (MQ=255)                                               gCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATCGATTAAGCAGAACTTGTGTGCGCATTAc                            >  1:701330/1‑81 (MQ=255)                                               gCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATCGATTAAGCAGAACTTGTGTGCGCATTAc                            >  2:701332/1‑81 (MQ=255)                                                           aGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATCGATTAAGCAGAACTTGTGTGCGCATTACCCGGGCTTGCAg                <  2:701299/81‑1 (MQ=255)                                                           aGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATCGATTAAGCAGAACTTGTGTGCGCATTACCCGGGCTTGCAg                >  2:701342/1‑81 (MQ=255)                                                             cATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATCGATTAAGCAGAACTTGTGTGCGCATTACCCGGGCTTGCAGGc              <  1:701300/81‑1 (MQ=255)                                                               tCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATCGATTAAGCAGAACTTGTGTGCGCATTACCCGGGCTTGCAGGCaa            <  2:701301/81‑1 (MQ=255)                                                                  aaCACATCTTTTAAAAAAAGATGTTTTTTCAATCGATTAAGCAGAACTTGTGTGCGCATTACCCGGGCTTGCAGGCaaaaa         <  1:701302/81‑1 (MQ=255)                                                                   acacATCTTTTAAAAAAAGATGTTTTTTCAATCGATTAAGCAGAACTTGTGTGCGCATTACCCGGGCTTGCAGGCaaaaaa        <  2:701303/81‑1 (MQ=255)                                                                   acacATCTTTTAAAAAAAGATGTTTTTTCAATCGATTAAGCAGAACTTGTGTGCGCATTACCCGGGCTTGCAGGCaaaa          >  2:701338/1‑79 (MQ=255)                                                                          ttttAAAAAAAGATGTTTTTTCAATCGATTAAGCAGAACTTGTGTGCGCATTACCCGGGCTTGCAGGCAAAAAAGAGATc  >  2:701358/1‑80 (MQ=255)                                                                             |                                                                             TGGGCGGTAGAGATCTACGTTTTCGCGAAAAAGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTAAAAAAAAGATGTTTTTTCAATCGATTAAGCAGAACTTGTGTGCGCATTACCCGGGCTTGCAGGCAAAAAAGAGATC  >  NC_000913/3863741‑3863893

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑