Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,913,188	T→G	I161L (ATC→CTC)	glmS ←	L‑glutamine‑‑D‑fructose‑6‑phosphate aminotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,913,188	0	T	G	100.0%	136.4 / NA	37	I161L (ATC→CTC)	glmS	L‑glutamine‑‑D‑fructose‑6‑phosphate aminotransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (27/10);  total (27/10)

GCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGTATCCGGGTGACGGGAGTCCATGATCACTGTACCGTACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGC  >  NC_000913/3913111‑3913264                                                                              |                                                                             gCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGTATCCGGGTGACGGGAGTCCATGAGCa                                                                            <  1:713433/80‑1 (MQ=255)    cATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGTATCCGGGTGACGGGAGTCCATGAGCACTGt                                                                        <  2:713434/81‑1 (MQ=255)     aTCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGTATCCGGGTGACGGGAGTCCATGAGCACTGTa                                                                       >  1:713479/1‑81 (MQ=255)       ccccAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGTATCCGGGTGACGGGAGTCCATGAGCACTGTAcc                                                                     >  1:713497/1‑81 (MQ=255)       ccccAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGTATCCGGGTGACGGGAGTCCATGAGCACTGTAcc                                                                     >  1:713478/1‑81 (MQ=255)        cccAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGTATCCGGGTGACGGGAGTCCATGAGCACTGTAcc                                                                     <  1:713435/80‑1 (MQ=255)            ggCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGTATCCGGGTGACGGGAGTCCATGAGCACTGTACCGTACg                                                                >  2:713487/1‑81 (MQ=255)            ggCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGTATCCGGGTGACGGGAGTCCATGAGCACTGTACCGTACg                                                                >  2:713483/1‑81 (MQ=255)             gCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGTATCCGGGTGACGGGAGTCCATGAGCACTGTACCGTACg                                                                >  1:713500/1‑80 (MQ=255)                   cACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGTATCCGGGTGACGGGAGTCCATGAGCACTGTACCGTACGCACCACg                                                         >  1:713499/1‑81 (MQ=255)                      cAGCGGACTACCAGAACGTGCCGCCAGCAGGGTATCCGGGTGACGGGAGTCCATGAGCACTGTACCGTACGCACCACGCAg                                                      >  1:713512/1‑81 (MQ=255)                        gCGGACTACCAGAACGTGCCGCCAGCAGGGTATCCGGGTGACGGGAGTCCATGAGCACTGTACCGTACGCACCACGCAGCt                                                    <  2:713436/81‑1 (MQ=255)                         cGGACTACCAGAACGTGCCGCCAGCAGGGTATCCGGGTGACGGGAGTCCATGAGCACTGTACCGTACGCACCACGCAGCTg                                                   <  1:713437/81‑1 (MQ=255)                         cGGACTACCAGAACGTGCCGCCAGCAGGGTATCCGGGTGACGGGAGTCCATGAGCACTGTACCGTACGCACCACGCAGCTg                                                   <  1:713438/81‑1 (MQ=255)                           gACTACCAGAACGTGCCGCCAGCAGGGTATCCGGGTGACGGGAGTCCATGAGCACTGTACCGTACGCACCACGCAGCTGCg                                                 >  1:713475/1‑81 (MQ=255)                               aCCAGAACGTGCCGCCAGCAGGGTATCCGGGTGACGGGAGTCCATGAGCACTGTACCGTACGCACCACGCAGCTGCGGGa                                              >  2:713481/1‑80 (MQ=255)                                  aGAACGTGCCGCCAGCAGGGTATCCGGGTGACGGGAGTCCATGAGCACTGTACCGTACGCACCACGCAGCTGCGGGATAg                                           >  2:713494/1‑80 (MQ=255)                                      cGTGCCGCCAGCAGGGTATCCGGGTGACGGGAGTCCATGAGCACTGTACCGTACGCACCACGCAGCTGCGGGATAGCACGc                                      <  1:713439/81‑1 (MQ=255)                                        tGCCGCCAGCAGGGTATCCGGGTGACGGGAGTCCATGAGCACTGTACCGTACGCACCACGCAGCTGCGGGATAGCACGCAg                                    <  1:713440/81‑1 (MQ=255)                                           cgccAGCAGGGTATCCGGGTGACGGGAGTCCATGAGCACTGTACCGTACGCACCACGCAGCTGCGGGATAGCACGCAGAAc                                 >  1:713493/1‑81 (MQ=255)                                            gccAGCAGGGTATCCGGGTGACGGGAGTCCATGAGCACTGTACCGTACGCACCACGCAGCTGCGGGATAGCACGCAGAACg                                >  2:713508/1‑81 (MQ=255)                                            gccAGCAGGGTATCCGGGTGACGGGAGTCCATGAGCACTGTACCGTACGCACCACGCAGCTGCGGGATAGCACGCAGAACg                                >  2:713480/1‑81 (MQ=255)                                            gccAGCAGGGTATCCGGGTGACGGGAGTCCATGAGCACTGTACCGTACGCACCACGCAGCTGCGGGATAGCACGCAGAACg                                >  1:713514/1‑81 (MQ=255)                                             ccAGCAGGGTATCCGGGTGACGGGAGTCCATGAGCACTGTACCGTACGCACCACGCAGCTGCGGGATAGCACGCAGAACgg                               >  1:713484/1‑81 (MQ=255)                                               agcagGGTATCCGGGTGACGGGAGTCCATGAGCACTGTACCGTACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGcc                             >  1:713488/1‑81 (MQ=255)                                               agcagGGTATCCGGGTGACGGGAGTCCATGAGCACTGTACCGTACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGcc                             >  2:713503/1‑81 (MQ=255)                                                  agGGTATCTGGGTGACGGGAGTCCATGAGCACTGTACCGTACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCa                          >  2:713491/1‑81 (MQ=255)                                                  agGGTATCCGGGTGACGGGAGTCCATGAGCACTGTACCGTACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCa                          <  1:713441/81‑1 (MQ=255)                                                       aTCCGGGTGACGGGAGTCCATGAGCACTGTACCGTACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACg                        >  1:713489/1‑78 (MQ=255)                                                             gTGACGGGAGTCCATGAGCACTGTACCGTACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTccc               <  2:713442/81‑1 (MQ=255)                                                                  gggAGTCCATGAGCACTGTACCGTACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCtt          >  1:713509/1‑81 (MQ=255)                                                                  gggAGTCCATGAGCACTGTACCGTACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCtt          >  1:713524/1‑81 (MQ=255)                                                                   ggAGTCCATGAGCACTGTACCGTACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTg         >  2:713501/1‑81 (MQ=255)                                                                    gAGTCCATGAGCACTGTACCGTACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGt        >  1:713506/1‑81 (MQ=255)                                                                      gTCCATGAGCACTGTACCGTACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGttt      >  2:832823/1‑81 (MQ=255)                                                                         cATGAGCACTGTACCGTACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAg   >  1:713510/1‑81 (MQ=255)                                                                          aTGAGCACTGTACCGTACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGc  >  2:713516/1‑81 (MQ=255)                                                                              |                                                                             GCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGTATCCGGGTGACGGGAGTCCATGATCACTGTACCGTACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGC  >  NC_000913/3913111‑3913264

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑