Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,917,491	T→A	K259* (AAA→TAA)	atpG ←	ATP synthase F1 complex subunit gamma

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,917,491	0	T	A	100.0%	133.8 / NA	35	K259* (AAA→TAA)	atpG	ATP synthase F1 complex subunit gamma
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (15/20);  total (15/20)

GCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAGGTTTT  >  NC_000913/3917415‑3917565                                                                             |                                                                           gcCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTAAATc                                                                        <  2:714646/81‑1 (MQ=255)    cccGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTAAATCAgg                                                                     <  2:714647/81‑1 (MQ=255)     ccGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCGGCTCTTAAATCAGGc                                                                    >  2:714692/1‑81 (MQ=255)       gagaCGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTAAATCAGGCTg                                                                  >  1:714704/1‑81 (MQ=255)          aCGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTAAATCAGGCTgccg                                                               >  2:714696/1‑81 (MQ=255)            gATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTAAATCAGGCTgccgc                                                              >  1:714695/1‑80 (MQ=255)               ctcGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTAAATCAGGCTGCCGCCAtt                                                          >  2:714699/1‑81 (MQ=255)                 cGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTAAATCAGGCTGCCGCCATTgt                                                        >  1:714708/1‑81 (MQ=255)                  ggTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTAAATCAGGCTGCCGCCAtt                                                          <  1:714652/78‑1 (MQ=255)                  ggTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTAAATCAGGCTGCCGCCAtt                                                          <  1:714650/78‑1 (MQ=255)                  ggTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTAAATCAGGCTGCCGCCAt                                                           <  2:714648/77‑1 (MQ=255)                  ggTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTAAATCAGGCTGCCGCCATTgtc                                                       <  1:714651/81‑1 (MQ=255)                  ggTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTAAATCAGGCTGCCGCCATTgt                                                        <  2:714649/80‑1 (MQ=255)                  ggTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTAAATCAGGCTGCCGCCATTg                                                         <  1:714653/79‑1 (MQ=255)                   gTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTAAATCAGGCTGCCGCCATTgtcg                                                      >  2:714745/1‑81 (MQ=255)                    tGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTAAATCAGGCTGCCGCCATTgtcgg                                                     <  1:714654/81‑1 (MQ=255)                      aGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTAAATCAGGCTGCCGCCATTgtcggtc                                                   >  1:714690/1‑81 (MQ=255)                       gTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTAAATCAGGCTGCCGCCATTgtcggtcg                                                  <  1:714655/81‑1 (MQ=255)                          ccTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTAAATCAGGCTGCCGCCATTGTCGGTCGCgg                                               >  2:714715/1‑81 (MQ=255)                                 aaTGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTAAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCAt                                        >  1:714702/1‑81 (MQ=255)                                  aTGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTAAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCAt                                        <  2:714656/80‑1 (MQ=255)                                     cTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTAAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGcc                                    <  1:714657/81‑1 (MQ=255)                                        gCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTAAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGccac                                  >  1:714698/1‑80 (MQ=255)                                            gACGAGCTTTGTTGTATACCAACTGCAGCTCTTAAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATAc                             >  1:714700/1‑81 (MQ=255)                                                 gCTTTGTTGTATACCAACTGCAGCTCTTAAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGggcg                        <  1:714658/81‑1 (MQ=255)                                                  cTTTGTTGTATACCAACTGCAGCTCTTAAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGggcgg                       <  2:714659/81‑1 (MQ=255)                                                          tataCCAACTGCAGCTCTTAAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTc                <  1:714660/80‑1 (MQ=255)                                                            taCCAACTGCAGCTCTTAAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCt             <  1:714662/81‑1 (MQ=255)                                                            taCCAACTGCAGCTCTTAAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCt             <  1:714661/81‑1 (MQ=255)                                                            taCCAACTGCAGCTCTTAAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCt             <  2:714663/81‑1 (MQ=255)                                                              ccAACTGCAGCTCTTAAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTgg           <  2:714664/81‑1 (MQ=255)                                                               cAACTGCAGCTCTTAAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGc          >  1:714713/1‑81 (MQ=255)                                                                 aCTGCAGCTCTTAAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGc          >  1:714703/1‑79 (MQ=255)                                                                   tGCAGCTCTTAAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAgg      >  2:714712/1‑81 (MQ=255)                                                                       gCTCTTAAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAGGtttt  <  1:714665/81‑1 (MQ=255)                                                                             |                                                                           GCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCATTGTCGGTCGCGGCTTTCATCGCCACCATACGGGCGGCCTGCTCGCTGGCCAGGTTTT  >  NC_000913/3917415‑3917565

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑