Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,941,643	T→G	intergenic (‑316/‑165)	yieP ← / → rrsC	putative transcriptional regulator YieP/16S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,941,643	0	T	G	100.0%	70.5 / NA	20	intergenic (‑316/‑165)	yieP/rrsC	putative transcriptional regulator YieP/16S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (12/8);  total (12/8)

CGGCAGAGAAAGCAAAAATAAATGCTTGACTCTGTAGCGGGAAAGCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTA  >  NC_000913/3941574‑3941720                                                                      |                                                                              cGGCAGAGAAAGCAAAAATAAATGCTTGACTCTGTAGCGGGAAAGCGTATTATGCACACCCCGCGCCGCGGAGAAagagc                                                                     >  1:720776/1‑80 (MQ=255)   gCAGAGAAAGCAAAAATAAATGCTTGACTCTGTAGCGGGAAAGCGTATTATGCACACCCCGCGCCGCGGAGAAagagcaaa                                                                  <  2:720745/81‑1 (MQ=255)      gagaAAGCAAAAATAAATGCTTGACTCTGTAGCGGGAAAGCGTATTATGCACACCCCGCGCCGCGGAGAAAGAGCAAAGCg                                                               >  2:720775/1‑81 (MQ=255)        gaAAGCAAAAATAAATGCTTGACTCTGTAGCGGGAAAGCGTATTATGCACACCCCGCGCCGCGGAGAAAGAGCAAAGCgg                                                              >  1:720772/1‑80 (MQ=255)           aGCAAAAATAAATGCTTGACTCTGTAGCGGGAAAGCGTATTATGCACACCCCGCGCCGCGGAGAAAGAGCAAAGCGGCACt                                                          <  1:720746/81‑1 (MQ=255)            gCAAAAATAAATGCTTGACTCTGTAGCGGGAAAGCGTATTATGCACACCCCGCGCCGCGGAGAAAGAGCAAAGCGGCACTg                                                         <  2:720747/81‑1 (MQ=255)              aaaaaTAAATGCTTGACTCTGTAGCGGGAAAGCGTATTATGCACACCCCGCGCCGCGGAGAAAGAGCAAAGCGGCACTGct                                                       >  2:720779/1‑81 (MQ=255)               aaaaTAAATGCTTGACTCTGTAGCGGGAAAGCGTATTATGCACACCCCGCGCCGCGGAGAAAGAGCAAAGCGGCACTGctc                                                      <  2:720748/81‑1 (MQ=255)                    aaatGCTTGACTCTGTAGCGGGAAAGCGTATTATGCACACCCCGCGCCGCGGAGAAAGAGCAAAGCGGCACTGCTCTTTaa                                                 >  2:720767/1‑81 (MQ=255)                     aatGCTTGACTCTGTAGCGGGAAAGCGTATTATGCACACCCCGCGCCGCGGAGAAAGAGCAAAGCGGCACTGCTCTTTaa                                                 <  2:720749/80‑1 (MQ=255)                         cTTGACTCTGTAGCGGGAAAGCGTATTATGCACACCCCGCGCCGCGGAGAAAGAGCAAAGCGGCACTGCTCTTTAACAAt                                             <  2:720750/80‑1 (MQ=255)                                    aGCGGGAAAGCGTATTATGCACACCCCGCGCCGCGGAGAAAGAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAAt                                 >  1:720783/1‑81 (MQ=255)                                        ggAAAGCGTATTATGCACACCCCGCGCCGCGGAGAAAGAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCtgt                             >  1:720790/1‑81 (MQ=255)                                        ggAAAGCGTATTATGCACACCCCGCGCCGCGGAGAAAGAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCtgt                             >  1:720769/1‑81 (MQ=255)                                             gCGTATTATGCACACCCCGCGCCGCGGAGAAAGAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTggg                        >  2:720774/1‑81 (MQ=255)                                               gTATTATGCACACCCCGCGCCGCGGAGAAAGAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCa                      >  1:720773/1‑81 (MQ=255)                                                  ttatGCACACCCCGCGCCGCGGAGAAAGAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTc                   <  1:720751/81‑1 (MQ=255)                                                       cacaCCCCGCGCCGCGGAGAAAGAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCg                  >  2:720782/1‑77 (MQ=255)                                                               gcgcCGCGGAGAAAGAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGAtt      <  1:720752/81‑1 (MQ=255)                                                                cgccgcGGAGAAAGAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTc     >  1:720778/1‑81 (MQ=255)                                                                   cgcgGAGAAAGAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCtt   >  1:720777/1‑80 (MQ=255)                                                                   cgcgGAGAAAGAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTa  <  2:720753/81‑1 (MQ=255)                                                                      |                                                                              CGGCAGAGAAAGCAAAAATAAATGCTTGACTCTGTAGCGGGAAAGCGTATTATGCACACCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTA  >  NC_000913/3941574‑3941720

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑