Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,988,602	G→T	V155V (GTC→GTA)	hemX ←	PF04375 family protein HemX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,988,602	0	G	T	100.0%	172.8 / NA	46	V155V (GTC→GTA)	hemX	PF04375 family protein HemX
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (20/26);  total (20/26)

GGTAATCAGACTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATCGGCCTGAGCCAGCAGCCAGGTTTTAG  >  NC_000913/3988530‑3988678                                                                         |                                                                             ggTAATCAGACTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTTACGTCCt                                                                       >  1:732566/1‑80 (MQ=255) ggTAATCAGACTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTTACGTCCTg                                                                      <  1:732504/81‑1 (MQ=255) ggTAATCAGACTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTTACGTCCTg                                                                      <  2:732505/81‑1 (MQ=255)   tAATCAGACTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTTACGTCCTGAt                                                                    <  2:732506/81‑1 (MQ=255)     aTCAGACTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTTACGTCCTGATc                                                                   <  1:732507/80‑1 (MQ=255)      tCAGACTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTTACGTCCTGATCGc                                                                 >  1:732576/1‑81 (MQ=255)        aGACTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTTACGTCCTGATCGCt                                                                <  1:732508/80‑1 (MQ=255)          aCTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTTACGTCCTGATCGCTCca                                                             >  2:732552/1‑81 (MQ=255)          aCTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTTACGTCCTGATCGCTCca                                                             >  2:732556/1‑81 (MQ=255)           cTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTTACGTCCTGATCGCTCcac                                                            <  2:732509/81‑1 (MQ=255)                   attcatATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTTACGTCCTGATCGCTCCACAGCTtccg                                                    >  1:732557/1‑81 (MQ=255)                   attcatATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTTACGTCCTGATCGCTCCACAGCTtcc                                                     <  2:732510/80‑1 (MQ=255)                     tcatATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTTACGTCCTGATCGCTCCACAGCTtccgtc                                                  >  1:732583/1‑81 (MQ=255)                       atatCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTTACGTCCTGATCGCTCCACAGCTtccgtccg                                                >  1:732575/1‑81 (MQ=255)                       atatCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTTACGTCCTGATCGCTCCACAGCTtccgtcc                                                 <  2:732511/80‑1 (MQ=255)                          tCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTTATGTCCTGATCGCTCCACAGCTTCCGTCCGGc                                              >  2:732560/1‑80 (MQ=255)                          tCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTTACGTCCTGATCGCTCCACAGCTTCCGTCCGGc                                              <  1:732512/80‑1 (MQ=255)                          tCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTTACGTCCTGATCGCTCCACAGCTTCCGTCCGGc                                              >  1:732585/1‑80 (MQ=255)                           ccGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTTACGTCCTGATCGCTCCACAGCTTCCGTCCGGc                                              >  2:732561/1‑79 (MQ=255)                            cGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTTACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAg                                           <  1:732513/81‑1 (MQ=255)                             gCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTTACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGt                                          >  1:732577/1‑81 (MQ=255)                               cAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTTACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGttt                                        <  2:732514/81‑1 (MQ=255)                               cAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTTACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGttt                                        >  2:732578/1‑81 (MQ=255)                                   cTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTTACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCAcc                                    <  1:732515/81‑1 (MQ=255)                                     ggCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTTACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCAc                                     >  2:732572/1‑78 (MQ=255)                                      gCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTTACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAg                                  <  2:732516/80‑1 (MQ=255)                                       cGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTTACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGa                                 >  1:732573/1‑80 (MQ=255)                                        gTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTTACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGaaa                               <  2:732517/81‑1 (MQ=255)                                        gTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTTACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGaaa                               <  1:732518/81‑1 (MQ=255)                                         tCTGCACTTTTCAGCAACGCTGCAGCGGTCGTTACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGaaaa                              <  1:732519/81‑1 (MQ=255)                                             cACTTTTCAGCAACGCTGCAGCGGTCGTTACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAAtg                            <  2:732520/79‑2 (MQ=255)                                             cACTTTTCAGCAACGCTGCAGCGGTCGTTACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATc                            <  1:732521/79‑1 (MQ=255)                                             cACTTTTCAGCAACGCTGCAGCGGTCGTTACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATCgg                          >  2:732564/1‑81 (MQ=255)                                                  ttCAGCAACGCTGCAGCGGTCGTTACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATCGGCCTg                      <  2:732522/80‑1 (MQ=255)                                                    cAGCAACGCTGCAGCGGTCGTTACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATCGGCCTGAg                    <  1:732523/80‑1 (MQ=255)                                                        aaCGCTGCAGCGGTCGTTACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATCGGCCTGAGCcagc               <  1:732525/81‑1 (MQ=255)                                                        aaCGCTGCAGCGGTCGTTACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATCGGCCTGAGCcagc               <  1:732524/81‑1 (MQ=255)                                                        aaCGCTGCAGCGGTCGTTACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATCGGCCTGAGCcagc               <  2:732526/81‑1 (MQ=255)                                                          cGCTGCAGCGGTCGTTACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATCGGCCTGAGCcagcag             >  2:732582/1‑81 (MQ=255)                                                               cAGCGGTCGTTACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATCGGCCTGAGCCAGCAGCCAgg        >  1:732571/1‑81 (MQ=255)                                                                aGCGGTCGTTACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATCGGCCTGAGCCAGCAGCCAGGt       >  2:732592/1‑81 (MQ=255)                                                                aGCGGTCGTTACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATCGGCCTGAGCCAGCAGCCAGGt       >  1:732579/1‑81 (MQ=255)                                                                 gCGGTCGTTACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATCGGCCTGAGCCAGCAGCCAGGtt      >  1:732591/1‑81 (MQ=255)                                                                  cGGTCGTTACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATCGGCCTGAGCCAGCAGCCAGGttt     <  1:732527/81‑1 (MQ=255)                                                                     tCGTTACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATCGGCCTGAGCCAGCAGCCAGGTTTTAg  <  2:732529/81‑1 (MQ=255)                                                                     gcGTTACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATCGGGCTGAGCCAGCAGCCAGGTTTTAg  <  2:732528/80‑1 (MQ=255)                                                                         |                                                                             GGTAATCAGACTCGGGTCATTCATATCCGCCAGGCTGGCGTCTGCACTTTTCAGCAACGCTGCAGCGGTCGTGACGTCCTGATCGCTCCACAGCTTCCGTCCGGCGAGTTTCACCAGAAAATCGGCCTGAGCCAGCAGCCAGGTTTTAG  >  NC_000913/3988530‑3988678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑