Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,098,551	A→G	G272G (GGA→GGG)	rhaS →	DNA‑binding transcriptional activator RhaS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,098,551	0	A	G	100.0%	107.8 / NA	30	G272G (GGA→GGG)	rhaS	DNA‑binding transcriptional activator RhaS
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/18);  total (12/18)

TTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACGCCATATTGCGAATAATCAACTTCGTTCTC  >  NC_000913/4098477‑4098628                                                                           |                                                                              ttCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGGCGGGAt                                                                         >  1:759557/1‑81 (MQ=255)  tCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGGCGGGATg                                                                        >  2:759559/1‑81 (MQ=255)    aGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGGCGGGATGGc                                                                      >  2:759549/1‑81 (MQ=255)           gTAACCACTTTTCGACGCTTTTTCGCCGAGAATTTAACTGGTCACCGCGTGATATTCGCCAGGGGCGGGATGGCTTTCTGc                                                               <  2:759502/81‑1 (MQ=255)              aCCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGGCGGGATGGCTTTCTGCaa                                                             <  1:759503/80‑1 (MQ=255)               ccACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGGCGGGATGGCTTTCTGCAAt                                                            >  2:759546/1‑80 (MQ=255)                cACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGGCGGGATGGCTTTCTGCAATaa                                                          >  1:759553/1‑81 (MQ=255)                 aCTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGGCGGGATGGCTTTCTGCAATAAc                                                         <  1:759505/81‑1 (MQ=255)                 aCTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGGCGGGATGGCTTTCTGCAATAAc                                                         <  2:759504/81‑1 (MQ=255)                      tCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGGCGGGATGGCTTTCTGCAATAACGCGa                                                     <  2:759506/80‑1 (MQ=255)                        gACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGGCGGGATGGCTTTCTGCAATAACGCGAAtc                                                  >  2:759564/1‑81 (MQ=255)                          cGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGGCGGGATGGCTTTCTGCAATAACGCGAAtctt                                                <  1:759507/81‑1 (MQ=255)                          cGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGGCGGGATGGCTTTCTGCAATAACGCGAAtctt                                                <  1:759508/81‑1 (MQ=255)                               tttCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGGCGGGATGGCTTTCTGCAATAACGCGAATCTTCTCaa                                           >  1:759565/1‑81 (MQ=255)                                 tCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGGCGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAAc                                          <  2:759509/80‑1 (MQ=255)                                  cGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGGCGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACg                                         <  2:759510/80‑1 (MQ=255)                                   gCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGGCGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGt                                        <  2:759511/80‑1 (MQ=255)                                      gagaGTTTAACTGGTCACCGCGTGATATTCGCCAGGGGCGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTAttt                                    >  1:759554/1‑81 (MQ=255)                                             tAACTGGTCACCGCGTGATATTCGCCAGGGGCGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACGc                              <  2:759512/80‑1 (MQ=255)                                                  ggTCACCGCGTGATATTCGCCAGGGGCGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACGCCATAtt                        >  2:759570/1‑81 (MQ=255)                                                      aCCGCGTGATATTCGCCAGGGGCGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACGCCATATTGCGa                    <  1:759513/81‑1 (MQ=255)                                                         gcgTGATATTCGCCAGGGGCGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACGCCATATTGCGaat                  >  2:759586/1‑80 (MQ=255)                                                           gTGATATTCGCCAGGGGCGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACGCCATATTGCGaataat               >  2:759550/1‑81 (MQ=255)                                                               tatTCGCCAGGGGCGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACGCCATATTGCGAATAATCAAc           <  1:759514/81‑1 (MQ=255)                                                                 ttCGCCAGGGGCGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACGCCATATTGCGAATAATCAACt          <  2:759515/80‑1 (MQ=255)                                                                  tCGCCAGGGGCGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACGCCATATTGCGAATAATCAACTTc        >  2:759572/1‑81 (MQ=255)                                                                   cGCCAGGGGCGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACGCCATATTGCGAATAATCAACTTc        <  2:759516/80‑1 (MQ=255)                                                                   cGCCAGGGGCGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACGCCATATTGCGAATAATCAACTTc        <  1:759517/80‑1 (MQ=255)                                                                     ccAGGGGCGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACGCCATATTGCGAATAATCAACTTCGtt     <  1:759518/81‑1 (MQ=255)                                                                     ccAGGGGCGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACGCCATATTGCGAATAATCAACTTCGtt     <  1:759519/81‑1 (MQ=255)                                                                        ggggCGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACGCCATATTGCGAATAATCAACTTCGTtctc  >  1:759571/1‑81 (MQ=255)                                                                        ggggCGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACGCCATATTGCGAATAATCAACTTCGTtctc  >  1:759551/1‑81 (MQ=255)                                                                           |                                                                              TTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACGCCATATTGCGAATAATCAACTTCGTTCTC  >  NC_000913/4098477‑4098628

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑