Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,175,484	G→T	noncoding (13/85 nt)	tyrU →	tRNA‑Tyr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,175,484	0	G	T	100.0%	137.2 / NA	36	noncoding (13/85 nt)	tyrU	tRNA‑Tyr
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (18/18);  total (18/18)

TAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTT  >  NC_000913/4175407‑4175560                                                                              |                                                                             tagAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCTAGc                                                                           >  2:778650/1‑81 (MQ=255)    agCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCTAGCGGc                                                                        <  1:778607/81‑1 (MQ=255)    agCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCTAGCGGc                                                                        <  2:778606/81‑1 (MQ=255)     gCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCTAGCGGcc                                                                       <  1:778608/81‑1 (MQ=255)     gCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCTAGCGGcc                                                                       <  1:778609/81‑1 (MQ=255)     gCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCTAGCGGc                                                                        >  1:778654/1‑80 (MQ=255)      cAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCTAGCGGcc                                                                       >  2:778669/1‑80 (MQ=255)            aCTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCTAGCGGCCAAAgg                                                                  >  1:778652/1‑79 (MQ=255)            aCTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCTAGCGGCCAAAGGGa                                                                <  1:778610/81‑1 (MQ=255)              ttGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCTAGCGGCCAAAGGGAg                                                               >  1:778668/1‑80 (MQ=255)                 tAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCTAGCGGCCAAAGGGAGCAg                                                            <  2:778612/80‑1 (MQ=255)                 tAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCTAGCGGCCAAAGGGAGCAg                                                            <  2:778611/80‑1 (MQ=255)                      aGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCTAGCGGCCAAAGGGAGCAGACTGt                                                       <  1:778613/80‑1 (MQ=255)                       gTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCTAGCGGCCAAAGGGAGCAGACTGTa                                                      >  2:778671/1‑80 (MQ=255)                          ggTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCTAGCGGCCAAAGGGAGCAGACTGTAAAt                                                   <  2:778614/80‑1 (MQ=255)                          ggTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCTAGCGGCCAAAGGGAGCAGACTGTAAATc                                                  <  1:778615/81‑1 (MQ=255)                                    tCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCTAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTca                                          >  1:778663/1‑79 (MQ=255)                                      gATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCTAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGa                                      >  1:778666/1‑81 (MQ=255)                                       aTTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCTAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGAc                                     >  1:778685/1‑81 (MQ=255)                                        ttCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCTAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACt                                    <  2:778616/81‑1 (MQ=255)                                        ttCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCTAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACt                                    <  1:778617/81‑1 (MQ=255)                                          ccGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCTAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTc                                  >  2:778672/1‑81 (MQ=255)                                             gTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCTAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCg                                 >  1:778680/1‑79 (MQ=255)                                              tAGTCGGCACCATCAAGTCCGGTGGGGTTCCCTAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAg                              <  1:778618/81‑1 (MQ=255)                                                   ggCACCATCAAGTCCGGTGGGGTTCCCTAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCg                         <  2:778619/81‑1 (MQ=255)                                                   ggCACCATCAAGTCCGGTGGGGTTCCCTAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCg                         <  2:778620/81‑1 (MQ=255)                                                            aaGTCCGGTGGGGTTCCCTAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTcc                >  1:778675/1‑81 (MQ=255)                                                             aGTCCGGTGGGGTTCCCTAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTccc               >  2:778677/1‑81 (MQ=255)                                                               tCCGGTGGGGTTCCCTAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTcccc              >  2:778679/1‑80 (MQ=255)                                                                 cGGTGGGGTTCCCTAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCccac           <  1:778621/81‑1 (MQ=255)                                                                  ggTGGGGTTCCCTAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCccac           >  1:778676/1‑80 (MQ=255)                                                                   gTGGGGTTCCCTAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCccacca         <  1:778622/81‑1 (MQ=255)                                                                   gTGGGGTTCCCTAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCccacca         >  2:778687/1‑81 (MQ=255)                                                                    tGGGGTTCCCTAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCccaccac        <  2:778623/81‑1 (MQ=255)                                                                      gggTTCCCTAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCccaccacc       >  2:778700/1‑80 (MQ=255)                                                                          tCCCTAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAAttt  >  2:778670/1‑81 (MQ=255)                                                                              |                                                                             TAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTT  >  NC_000913/4175407‑4175560

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑