Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,184,579	T→G	I1112S (ATC→AGC)	rpoB →	RNA polymerase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,184,579	0	T	G	100.0%	139.5 / NA	39	I1112S (ATC→AGC)	rpoB	RNA polymerase subunit beta
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (23/16);  total (23/16)

TACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAG  >  NC_000913/4184503‑4184656                                                                             |                                                                              tACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGAGCCTc                                                                           >  2:780995/1‑81 (MQ=255)  aCGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGAGCCt                                                                            <  2:780947/79‑1 (MQ=255)   cGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGAGCCTCg                                                                          >  2:781018/1‑80 (MQ=255)    gATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGAGCCTCGa                                                                         >  1:781026/1‑80 (MQ=255)        aaaaCGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGAGCCTCGAAAccc                                                                    <  1:780948/81‑1 (MQ=255)            cGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGAGCCTCGAAACCCACCt                                                                >  2:781015/1‑81 (MQ=255)             ggTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGAGCCTCGAAACCCACCTg                                                               >  1:781021/1‑81 (MQ=255)                aCGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGAGCCTCGAAACCCACCTggg                                                             >  1:781005/1‑80 (MQ=255)                 cGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGAGCCTCGAAACCCACCTGGGt                                                            <  1:780949/80‑1 (MQ=255)                   ccGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGAGCCTCGAAACCCACCTGGGTATg                                                         >  2:781023/1‑81 (MQ=255)                    cGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGAGCCTCGAAACCCACCTGGGTATgg                                                        >  2:781034/1‑81 (MQ=255)                     ggTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGAGCCTCGAAACCCACCTGGGTATGGc                                                       <  1:780950/81‑1 (MQ=255)                         gACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGAGCCTCGAAACCCACCTGGGTATGGCTGc                                                    >  1:781022/1‑80 (MQ=255)                            aTCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGAGCCTCGAAACCCACCTGGGTATGGCTGCGa                                                  >  1:781029/1‑79 (MQ=255)                               gTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGAGCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGt                                             <  2:780951/81‑1 (MQ=255)                                tACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGAGCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTa                                            >  1:781028/1‑81 (MQ=255)                                  cTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGAGCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTAt                                           <  2:780952/80‑1 (MQ=255)                                     aaCCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGAGCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGc                                       <  2:780953/81‑1 (MQ=255)                                     aaCCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGAGCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGc                                       <  1:780954/81‑1 (MQ=255)                                       cccGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGAGCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGa                                     >  2:781010/1‑81 (MQ=255)                                          gCTGGGCGTACCGTCTCGTATGAACATCGGTCAGAGCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGAc                                    >  1:781033/1‑79 (MQ=255)                                             gggCGTACCGTCTCGTATGAACATCGGTCAGAGCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGAc                                    >  2:781038/1‑76 (MQ=255)                                             gggCGTACCGTCTCGTATGAACATCGGTCAGAGCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGAc                                    >  2:781040/1‑76 (MQ=255)                                              ggCGTACCGTCTCGTATGAACATCGGTCAGAGCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGa                                <  2:780955/79‑1 (MQ=255)                                                 gTACCGTCTCGTATGAACATCGGTCAGAGCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAAc                           >  1:781035/1‑81 (MQ=255)                                                   aCCGTCTCGTATGAACATCGGTCAGAGCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGc                         >  2:781012/1‑81 (MQ=255)                                                    ccGTCTCGTATGAACATCGGTCAGAGCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGcc                        <  2:780956/81‑1 (MQ=255)                                                    ccGTCTCGTATGAACATCGGTCAGAGCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGcc                        <  1:780957/81‑1 (MQ=255)                                                    cagTCTCGTATGAACATCGGTCAGAGCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGc                         <  2:780958/78‑1 (MQ=255)                                                     cGTCTCGTATGAACATCGGTCAGAGCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCa                       >  1:781048/1‑81 (MQ=255)                                                       tctcGTATGAACATCGGTCAGAGCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATg                     >  2:781062/1‑81 (MQ=255)                                                           gTATGAACATCGGTCAGAGCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCt                   <  2:780959/79‑1 (MQ=255)                                                            tATGAACATCGGTCAGAGCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGaa                <  2:780960/81‑1 (MQ=255)                                                              tGAACATCGGTCAGAGCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAc              <  2:780961/81‑1 (MQ=255)                                                                   aTCGGTCAGAGCCTCGAAGCCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAcagcag         >  2:781061/1‑81 (MQ=255)                                                                   aTCGGTCAGAGCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAcagcag         <  2:780962/81‑1 (MQ=255)                                                                     cGGTCAGAGCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACagcagc        >  1:781053/1‑80 (MQ=255)                                                                      ggTCAGAGCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACagcagc        >  2:781042/1‑79 (MQ=255)                                                                       gTCAGAGCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACagcagc        >  1:781047/1‑78 (MQ=255)                                                                          agagCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaagaag  >  2:781044/1‑81 (MQ=255)                                                                             |                                                                              TACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAG  >  NC_000913/4184503‑4184656

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑