Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,299,439	T→C	intergenic (‑73/+125)	fdhF ← / ← mdtP	formate dehydrogenase H/putative multidrug efflux pump outer membrane channel

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,299,439	0	T	C	100.0%	149.5 / NA	41	intergenic (‑73/+125)	fdhF/mdtP	formate dehydrogenase H/putative multidrug efflux pump outer membrane channel
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/17);  total (24/17)

TCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTT  >  NC_000913/4299363‑4299513                                                                             |                                                                           tCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTCAtgt                                                                        >  2:808625/1‑81 (MQ=255)   aTCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTCATGTgg                                                                      <  1:808576/81‑1 (MQ=255)         ctcGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTCATGTGGGGTCAt                                                                >  2:808636/1‑81 (MQ=255)           cGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTCATGTGGGGTCATTc                                                              >  2:808637/1‑81 (MQ=255)           cGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTCATGTGGGGTCATTc                                                              >  2:808621/1‑81 (MQ=255)            gCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTCATGTGGGGTCAt                                                                <  1:808577/78‑1 (MQ=255)            gCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTCATGTGGGGTCATTcc                                                             >  2:808632/1‑81 (MQ=255)             cTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTCATGTGGGGTCATTccc                                                            >  1:808635/1‑81 (MQ=255)                cAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTCATGTGGGGTCATTCCCt                                                           <  1:808578/79‑1 (MQ=255)                cAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTCATGTGGGGTCATTCCCTGa                                                         >  2:808624/1‑81 (MQ=255)                 aGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTCATGTGGGGTCATTCCCTGat                                                        <  1:808579/81‑1 (MQ=255)                 aGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTCATGTGGGGTCATTCCCTGat                                                        <  2:808580/81‑1 (MQ=255)                    tAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTCATGTGGGGTCATTCCCTGatat                                                      >  2:808581/1‑80 (MQ=255)                     aatcaaatcaCGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTCATGTGGGGTCATTCCCTGATATTa                                                    >  1:808633/1‑81 (MQ=255)                     aatcaaatcaCGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTCATGTGGGGTCATTCCCTGATATTa                                                    >  1:808620/1‑81 (MQ=255)                            tcaCGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTCATGTGGGGTCATTCCCTGATATTACGGGCAc                                             <  2:808582/81‑1 (MQ=255)                              aCGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTCATGTGGGGTCATTCCCTGATATTACGGGCACt                                            <  1:808583/80‑1 (MQ=255)                              aCGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTCATGTGGGGTCATTCCCTGATATTACGGGCACt                                            <  2:808584/80‑1 (MQ=255)                               cGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTCATGTGGGGTCATTCCCTGATATTACGGGCACtat                                          >  1:808622/1‑81 (MQ=255)                                 cATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTCATGTGGGGTCATTCCCTGATATTACGGGCACtattt                                        <  2:808585/81‑1 (MQ=255)                                 cATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTCATGTGGGGTCATTCCCTGATATTACGGGCACtattt                                        <  2:808586/81‑1 (MQ=255)                                 cATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTCATGTGGGGTCATTCCCTGATATTACGGGCACtatt                                         >  2:808629/1‑80 (MQ=255)                                  aTACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTCATGTGGGGTCATTCCCTGATATTACGGGCACtattta                                       >  2:808644/1‑81 (MQ=255)                                  aTACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTCATGTGGGGTCATTCCCTGATATTACGGGCACtattta                                       >  1:808642/1‑81 (MQ=255)                                   tACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTCATGTGGGGTCATTCCCTGATATTACGGGCACtatttat                                      >  1:808634/1‑81 (MQ=255)                                   tACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTCATGTGGGGTCATTCCCTGATATTACGGGCACtatttat                                      >  2:808646/1‑81 (MQ=255)                                       cgcTCTCGACTACAGTATGCATCTTTTATGCCACATTTCATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCaa                                  <  1:808587/81‑1 (MQ=255)                                        gcTCTCGACTACAGTATGCATCTTTTATGCCACATTTCATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCaaa                                 <  1:808588/81‑1 (MQ=255)                                          tctcGACTACAGTATGCATCTTTTATGCCACATTTCATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAAc                               >  2:808658/1‑81 (MQ=255)                                          tctcGACTACAGTATGCATCTTTTATGCCACATTTCATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAAc                               >  1:808638/1‑81 (MQ=255)                                            tcGACTACAGTATGCATCTTTTATGCCACATTTCATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAActc                             >  1:808628/1‑81 (MQ=255)                                                cTACAGTATGCATCTTTTATGCCACATTTCATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGAc                         <  2:808589/81‑1 (MQ=255)                                                 tACAGTATGCATCTTTTATGCCACATTTCATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGAc                         >  1:808630/1‑80 (MQ=255)                                                  aCAGTATGCATCTTTTATGCCACATTTCATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACg                        >  2:808640/1‑80 (MQ=255)                                                             cTTTTATGCCACATTTCATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTg            >  1:808631/1‑81 (MQ=255)                                                                ttATGCCACATTTCATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTgtcg         >  2:808643/1‑81 (MQ=255)                                                                ttATGCCACATTTCATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTgt           <  1:808592/79‑1 (MQ=255)                                                                ttATGCCACATTTCATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTgt           <  1:808590/79‑1 (MQ=255)                                                                ttATGCCACATTTCATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTgt           <  2:808591/79‑1 (MQ=255)                                                                  aTGCCACATTTCATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTgtcgt        >  2:808639/1‑80 (MQ=255)                                                                       acaTTTCATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGttt  <  2:808593/81‑1 (MQ=255)                                                                             |                                                                           TCATCGGTCTCGCTCCAGTTAATCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTT  >  NC_000913/4299363‑4299513

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑