Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,371,109	G→A	V29I (GTT→ATT)	groL →	chaperonin GroEL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,371,109	0	G	A	100.0%	121.3 / NA	32	V29I (GTT→ATT)	groL	chaperonin GroEL
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/15);  total (17/15)

AAAGACGTAAAATTCGGTAACGACGCTCGTGTGAAAATGCTGCGCGGCGTAAACGTACTGGCAGATGCAGTGAAAGTTACCCTCGGTCCAAAAGGCCGTAACGTAGTTCTGGATAAATCTTTCGGTGCACCGACCATCACCAAAGATGGTGT  >  NC_000913/4371034‑4371185                                                                            |                                                                             aaaGACGTAAAATTCGGTAACGACGCTCGTGTGAAAATGCTGCGCGGCGTAAACGTACTGGCAGATGCAGTGAAAATTAcc                                                                         >  1:825149/1‑81 (MQ=255)          aaaTTCGGTAACGACGCTCGTGTGAAAATGCTGCGCGGCGTAAACGTACTGGCAGATGCAGTGAAAATTACCCTCGGTcc                                                                 <  1:825103/80‑1 (MQ=255)          aaaTTCGGTAACGACGCTCGTGTGAAAATGCTGCGCGGCGTAAACGTACTGGCAGATGCAGTGAAAATTACCCTCGGTcc                                                                 <  1:825102/80‑1 (MQ=255)          aaaTTCGGTAACGACGCTCGTGTGAAAATGCTGCGCGGCGTAAACGTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCa                                                                <  2:825104/81‑1 (MQ=255)             ttCGGTAACGACGCTCGTGTGAAAATGCTGCGCGGCGTAAACGTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCaaaa                                                             <  1:825105/81‑1 (MQ=255)               cGGTAACGACGCTCGTGTGAAAATGCTGCGCGGCGTAAACGTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAgg                                                           >  2:825143/1‑81 (MQ=255)               cGGTAACGACGCTCGTGTGAAAATGCTGCGCGGCGTAAACGTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAgg                                                           >  1:825157/1‑81 (MQ=255)                ggTAACGACGCTCGTGTGAAAATGCTGCGCGGCGTAAACGTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGc                                                          <  1:825106/81‑1 (MQ=255)                ggTAACGACGCTCGTGTGAAAATGCTGCGCGGCGTAAACGTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGc                                                          >  1:825136/1‑81 (MQ=255)                   aaCGACGCTCGTGTGAAAATGCTGCGCGGCGTAAACGTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCCg                                                        >  2:825153/1‑80 (MQ=255)                   aaCGACGCTCGTGTGAAAATGCTGCGCGGCGTAAACGTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCCg                                                        >  2:825147/1‑80 (MQ=255)                     cgacgCTCGTGTGAAAATGCTGCGCGGCGTAAACGTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCCGTaa                                                     <  1:825107/81‑1 (MQ=255)                      gacgCTCGTGTGAAAATGCTGCGCGGCGTAAACGTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCCGTAAc                                                    <  2:825108/81‑1 (MQ=255)                       acgCTCGTGTGAAAATGCTGCGCGGCGTAAACGTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCCGTAACg                                                   >  2:825196/1‑81 (MQ=255)                             gtgtGAAAATGCTGCGCGGCGTAAACGTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCCGTAACGTAGt                                               <  1:825109/79‑1 (MQ=255)                                tgAAAATGCTGCGCGGCGTAAACGTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCCGTAACGTAGTTCTgg                                          <  1:825110/81‑1 (MQ=255)                                  aaaaTGCTGCGCGGCGTAAACGTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCCGTAACGTAGTTCTGGa                                         <  1:825112/80‑1 (MQ=255)                                  aaaaTGCTGCGCGGCGTAAACGTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCCGTAACGTAGTTCTGGAt                                        <  2:825111/81‑1 (MQ=255)                                         tgcGCGGCGTAAACGTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCCGTAACGTAGTTCTGGATAAATCtt                                 >  2:825158/1‑81 (MQ=255)                                          gcgcGGCGTAAACGTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCCGTAACGTAGTTCTGGATAAATCttt                                >  2:825146/1‑81 (MQ=255)                                              ggcgTAAACGTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCCGTAACGTAGTTCTGGATAAATCTTTCGGt                            <  1:825113/81‑1 (MQ=255)                                                cgTAAACGTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCCGTAACGTAGTTCTGGATAAATCTTTCGGTg                           >  1:825156/1‑80 (MQ=255)                                                  tAAACGTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCCGTAACGTAGTTCTGGATAAATCTTTCGGTGCAc                        <  1:825114/81‑1 (MQ=255)                                                      cGTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCCGTAACGTAGTTCTGGATAAATCTTTCGGTGCACCGAc                    >  1:825169/1‑81 (MQ=255)                                                      cGTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCCGTAACGTAGTTCTGGATAAATCTTTCGGTGCACCGAc                    >  1:825154/1‑81 (MQ=255)                                                       gTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCCGTAACGTAGTTCTGGATAAATCTTTCGGTGCACCGAcc                   <  1:825115/81‑1 (MQ=255)                                                       gTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCCGTAACGTAGTTCTGGATAAATCTTTCGGTGCACCGAc                    >  1:825152/1‑80 (MQ=255)                                                       gTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCCGTAACGTAGTTCTGGATAAATCTTTCGGTGCACCGAc                    >  2:825168/1‑80 (MQ=255)                                                               aGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCCGTAACGTAGTTCTGGATAAATCTTTCGGTGCACCGACCATCACCaa           <  1:825116/81‑1 (MQ=255)                                                               aGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCCGTAACGTAGTTCTGGATAAATCTTTCGGTGCACCGACCATCACCa            >  2:825165/1‑80 (MQ=255)                                                                     aGTGAAAATTACCCTCGGTCCAAAAGGCCGTAACGTAGTTCTGGATAAATCTTTCGGTGCACCGACCATCACCAAAGATgg     >  1:825173/1‑81 (MQ=255)                                                                        gAAAATTACCCTCGGTCCAAAAGGCCGTAACGTAGTTCTGGATAAATCTTTCGGTGCACCGACCATCACCAAAGATGgtgt  >  2:825160/1‑81 (MQ=255)                                                                            |                                                                             AAAGACGTAAAATTCGGTAACGACGCTCGTGTGAAAATGCTGCGCGGCGTAAACGTACTGGCAGATGCAGTGAAAGTTACCCTCGGTCCAAAAGGCCGTAACGTAGTTCTGGATAAATCTTTCGGTGCACCGACCATCACCAAAGATGGTGT  >  NC_000913/4371034‑4371185

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑