Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,503,704	A→G	intergenic (‑273/‑354)	insG ← / → yjhB	KpLE2 phage‑like element; IS4 putative transposase/putative sialic acid transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,503,704	0	A	G	100.0%	83.9 / NA	24	intergenic (‑273/‑354)	insG/yjhB	KpLE2 phage‑like element; IS4 putative transposase/putative sialic acid transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/15);  total (9/15)

TATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAACCTCTCAGTCTTTCCTCATTTGACGAAGGGAGTTTTATTCAACCTGAACGGACTACGAAAATGAGCACAAT  >  NC_000913/4503626‑4503775                                                                               |                                                                        tataACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAGcc                                                                       <  2:854814/81‑1 (MQ=255)   taACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATATCGTAAGCTAAAGTAATAGCctc                                                                     <  1:854815/81‑1 (MQ=255)             aaaGGTATACAGAAAAGCAAAGAATTACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAGCCTCTCAGTCTTTc                                                           >  1:854842/1‑81 (MQ=255)              aaGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAGCCTCTCAGTCTTTcc                                                          >  1:854848/1‑81 (MQ=255)                    taCAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAGCCTCTCAGTCTTTCCTCAttt                                                    <  1:854816/81‑1 (MQ=255)                    taCAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAGCCTCTCAGTCTTTCCTCAttt                                                    <  1:854817/81‑1 (MQ=255)                     aCAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAGCCTCTCAGTCTTTCCTCAttt                                                    >  2:854846/1‑80 (MQ=255)                       aGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAGCCTCTCAGTCTTTCCTCATTTGAc                                                 <  2:854818/81‑1 (MQ=255)                                 aGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAGCCTCTCAGTCTTTCCTCATTTGACGAAGGGAGtt                                       <  1:875951/81‑1 (MQ=255)                                        cTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAGCCTCTCAGTCTTTCCTCATTTGACGAAGGGAGTTTTATTCa                                <  2:854820/81‑1 (MQ=255)                                           cAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAGCCTCTCAGTCTTTCCTCATTTGACGAAGGGAGTTTTATTCAAcc                             >  1:854847/1‑81 (MQ=255)                                           cAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAGCCTCTCAGTCTTTCCTCATTTGACGAAGGGAGTTTTATTCAAcc                             <  1:854821/81‑1 (MQ=255)                                            aaaGGAAAACAGCTATAACGTAAGCTAAAGTAATAGCCTCTCAGTCTTTCCTCATTTGACGAAGGGAGTTTTATTCAACCt                            >  1:854851/1‑81 (MQ=255)                                             aaGGAAAACAGCTATAACGTAAGCTAAAGTAATAGCCTCTCAGTCTTTCCTCATTTGACGAAGGGAGTTTTATTCAACCTg                           <  2:854822/81‑1 (MQ=255)                                                 aaaaCAGCTATAACGTAAGCTAAAGTAATAGCCTCTCAGTCTTTCCTCATTTGACGAAGGGAGTTTTATTCAACCTGAACg                       <  2:854823/81‑1 (MQ=255)                                                 aaaaCAGCTATAACGTAAGCTAAAGTAATAGCCTCTCAGTCTTTCCTCATTTGACGAAGGGAGTTTTATTCAACCTGAACg                       >  2:854838/1‑81 (MQ=255)                                                 aaaaCAGCTATAACGTAAGCTAAAGTAATAGCCTCTCAGTCTTTCCTCATTTGACGAAGGGAGTTTTATTCAACCTGAACg                       >  2:854850/1‑81 (MQ=255)                                                  aaaCAGCTATAACGTAAGCTAAAGTAATAGCCTCTCAGTCTTTCCTCATTTGACGAAGGGAGTTTTATTCAACCTGAACgg                      <  1:854824/81‑1 (MQ=255)                                                    aCAGCTATAACGTAAGCTAAAGTAATAGCCTCTCAGTCTTTCCTCATTTGACGAAGGGAGTTTTATTCAACCTGAACGGAc                    <  1:854825/81‑1 (MQ=255)                                                       gCTATAACGTAAGCTAAAGTAATAGCCTCTCAGTCTTTCCTCATTTGACGAAGGGAGTTTTATTCAACCTGAACGGACTAc                 >  1:854863/1‑81 (MQ=255)                                                              cGTAAGCTAAAGTAATAGCCTCTCAGTCTTTCCTCATTTGACGAAGGGAGTTTTATTCAACCTGAACGGACTACGAAAATg          <  2:854826/81‑1 (MQ=255)                                                                  aGCTAAAGTAATAGCCTCTCAGTCTTTCCTCATTTGACGAAGGGAGTTTTATTCAACCTGAACGGACTACGAAAATGAGca      >  1:854858/1‑81 (MQ=255)                                                                       aaGTAATAGCCTCTCAGTCTTTCCTCATTTGACGAAGGGAGTTTTATTCAACCTGAACGGACTACGAAAATGAGCACAAt  <  2:854827/80‑1 (MQ=255)                                                                       aaGTAATAGCCTCTCAGTCTTTCCTCATTTGACGAAGGGAGTTTTATTCAACCTGAACGGACTACGAAAATGAGCACAAt  <  1:854828/80‑1 (MQ=255)                                                                               |                                                                        TATAACAGGTATAAAGGTATACAGAAAAGCAAAGAAATACTGCAAAGGAAAACAGCTATAACGTAAGCTAAAGTAATAACCTCTCAGTCTTTCCTCATTTGACGAAGGGAGTTTTATTCAACCTGAACGGACTACGAAAATGAGCACAAT  >  NC_000913/4503626‑4503775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑