Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	341,434	C→G	P104A (CCG→GCG)	yahJ →	putative deaminase with metallo‑dependent hydrolase domain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	341,434	0	C	G	100.0%	115.3 / NA	33	P104A (CCG→GCG)	yahJ	putative deaminase with metallo‑dependent hydrolase domain
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (12/21);  total (12/21)

CGCGCCAGACCGTGGAGATTCAGGACGGCAAAATTGTCGCCCTGCGCGAGAACAAGCTGCATCCGGACGCCACGCTGCCGCACTATGACGCTGGCGGTAAGCTGATGCTGCCCACCACCCGCGACATGCATATTCATCTCGACAAAACCTTTTA  >  NC_000913/341357‑341510                                                                              |                                                                             cgcgcCAGACCGTGGAGATTCAGGACGGCAAAATTGTCGCCCTGCGCGAGAACAAGCTGCATCCGGACGCCACGCTGgcgc                                                                           <  1:70622/81‑1 (MQ=255)  gcgcCAGACCGTGGAGATTCAGGACGGCAAAATTGTCGCCCTGCGCGAGAACAAGCTGCATCCGGACGCCACGCTGGCGCa                                                                          <  1:70623/81‑1 (MQ=255)      cAGACCGTGGAGATTCAGGACGGCAAAATTGTCGCCCTGCGCGAGAACAAGCTGCATCCGGACGTCACGCTGGCGCACTAt                                                                      <  2:70624/81‑1 (MQ=255)      cAGACCGTGGAGATTCAGGACGGCAAAATTGTCGCCCTGCGCGAGAACAAGCTGCATCCGGACGCCACGCTGGCGCACTAt                                                                      >  2:70679/1‑81 (MQ=255)            gTGGAGATTCAGGTCGGCAAAATTGTCGCCCTGCGCGAGAACAAGCTGCATCCGGTCGCCCCGCTGGCGCACCACGACGCt                                                                >  2:893823/1‑81 (MQ=255)                 gaTTCAGGACGGCAAAATTGTCGCCCTGCGCGAGAACAAGCTGCATCCGGACGCCACGCTGGCGCACTATGACGCTGGCgg                                                           >  1:70673/1‑81 (MQ=255)                  aTTCAGGACGGCAAAATTGTCGCCCTGCGCGAGAACAAGCTGCATCCGGACGCCACGCTGGCGCACTATGACGCTGGCgg                                                           >  1:70686/1‑80 (MQ=255)                  aTTCAGGACGGCAAAATTGTCGCCCTGCGCGAGAACAAGCTGCATCCGGACGCCACGCTGGCGCACTATGACGCTGGCGGt                                                          <  1:70625/81‑1 (MQ=255)                  aTTCAGGACGGCAAAATTGTCGCCCTGCGCGAGAACAAGCTGCATCCGGACGCCACGCTGGCGCACTATGACGCTGGCGGt                                                          <  1:70626/81‑1 (MQ=255)                        gACGGCAAAATTGTCGCCCTGCGCGAGAACAAGCTGCATCCGGACGCCACGCTGGCGCACTATGACGCTGGCGGTAAGCTg                                                    <  2:70627/81‑1 (MQ=255)                           ggCAAAATTGTCGCCCTGCGCGAGAACAAGCTGCATCCGGACGCCACGCTGGCGCACTATGACGCTGGCGGTAAGCTGAtg                                                 <  2:70628/81‑1 (MQ=255)                            gCAAAATTGTCGCCCTGCGCGAGAACAAGCTGCATCCGGACGCCACGCTGGCGCACTATGACGCTGGCGGTAAGCTGAtgc                                                <  2:70629/81‑1 (MQ=255)                                     tCGCCCTGCGCGAGAACAAGCTGCATCCGGACGCCACGCTGGCGCACTATGACGCTGGCGGTAAGCTGATGCTGCccacca                                       >  1:70675/1‑81 (MQ=255)                                     tCGCCCTGCGCGAGAACAAGCTGCATCCGGACGCCACGCTGGCGCACTATGACGCTGGCGGTAAGCTGATGCTGCccacc                                        <  2:70632/80‑1 (MQ=255)                                     tCGCCCTGCGCGAGAACAAGCTGCATCCGGACGCCACGCTGGCGCACTATGACGCTGGCGGTAAGCTGATGCTGCccacc                                        <  2:70630/80‑1 (MQ=255)                                     tCGCCCTGCGCGAGAACAAGCTGCATCCGGACGCCACGCTGGCGCACTATGACGCTGGCGGTAAGCTGATGCTGCccacc                                        <  1:70631/80‑1 (MQ=255)                                       gCCCTGCGCGAGAACAAGCTGCATCCGGACGCCACGCTGGCGCACTATGACGCTGGCGGTAAGCTGATGCTGCCcaccac                                      <  1:70633/80‑1 (MQ=255)                                        cccTGCGCGAGAACAAGCTGCATCCGGACGCCACGCTGGCGCACTATGACGCTGGCGGTAAGCTGATGCTGCCCACCAccc                                    <  1:70634/81‑1 (MQ=255)                                         ccTGCGCGAGAACAAGCTGCATCCGGACGCCACGCTGGCGCACTATGACGCTGGCGGTAAGCTGATGCTGCCCACCACCcg                                   >  1:70683/1‑81 (MQ=255)                                             cgcgAGAACAAGCTGCATCCGGACGCCACGCTGGCGCACTATGACGCTGGCGGTAAGCTGATGCTGCCCACCACCCGCGa                                >  1:70687/1‑80 (MQ=255)                                             cgcgAGAACAAGCTGCATCCGGACGCCACGCTGGCGCACTATGACGCTGGCGGTAAGCTGATGCTGCCCACCACCCGCGAc                               <  2:70636/81‑1 (MQ=255)                                             cgcgAGAACAAGCTGCATCCGGACGCCACGCTGGCGCACTATGACGCTGGCGGTAAGCTGATGCTGCCCACCACCCGCGAc                               <  1:70635/81‑1 (MQ=255)                                               cgAGAACAAGCTGCATCCGGACGCCACGCTGGCGCACTATGACGCTGGCGGTAAGCTGATGCTGCCCACCACCCGCGACAt                             <  2:70637/81‑1 (MQ=255)                                                gagaACAAGCTGCATCCGGACGCCACGCTGGCGCACTATGACGCTGGCGGTAAGCTGATGCTGCCCACCACCCGCGACATg                            >  1:70685/1‑81 (MQ=255)                                                gagaACAAGCTGCATCCGGACGCCACGCTGGCGCACTATGACGCTGGCGGTAAGCTGATGCTGCCCACCACCCGCGACATg                            >  1:70678/1‑81 (MQ=255)                                                 agaACAAGCTGCATCCGGACGCCACGCTGGCGCACTATGACGCTGGCGGTAAGCTGATGCTGCCCACCACCCGCGACATGc                           <  1:70638/81‑1 (MQ=255)                                                         cTGCATCCGGACGCCACGCTGGCGCACTATGACGCTGGCGGTAAGCTGATGCTGCCCACCACCCGCGACATGCATATTCAt                   <  2:70639/81‑1 (MQ=255)                                                             aTCCGGACGCCACGCTGGCGCACTATGACGCTGGCGGTAAGCTGATGCTGCCCACCACCCGCGACATGCATATTCATCTCg               <  2:70640/81‑1 (MQ=255)                                                                cGGACGCCACGCTGGCGCACTATGACGCTGGCGGTAAGCTGGTGCTGCCCACCACCCGCGACATGCATATTCATCTCGACa            <  1:133764/81‑1 (MQ=255)                                                                   aCGCCACGCTGGCGCACTATGACGCTGGCGGTAAGCTGATGCTGCCCACCACCCGCGACATGCATATTCATCTCGACaaaa         >  1:70684/1‑81 (MQ=255)                                                                     gCCACGCTGGCGCACTATGACGCTGGCGGTAAGCTGATGCTGCCCACCACCCGCGACATGCATATTCATCTCGACAAAAcc       >  2:70693/1‑81 (MQ=255)                                                                        aCGCTGGCGCACTATGACGCTGGCGGTAAGCTGATGCTGCCCACCACCCGCGACATGCATATTCATCTCGACAAAACCttt    >  1:70689/1‑81 (MQ=255)                                                                         cGCTGGCGCACTATGACGCTGGCGGTAAGCTGATGCTGCCCACCACCCGCGACATGCATATTCATCTCGACAAAACCtttt   <  1:70641/81‑1 (MQ=255)                                                                          gCTGGCGCACTATGACGCTGGCGGTAAGCTGATGCTGCCCACCACCCGCGACATGCATATTCATCTCGACAAAACCTTTTa  <  1:70642/81‑1 (MQ=255)                                                                              |                                                                             CGCGCCAGACCGTGGAGATTCAGGACGGCAAAATTGTCGCCCTGCGCGAGAACAAGCTGCATCCGGACGCCACGCTGCCGCACTATGACGCTGGCGGTAAGCTGATGCTGCCCACCACCCGCGACATGCATATTCATCTCGACAAAACCTTTTA  >  NC_000913/341357‑341510

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑