Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,638,175	A→G	intergenic (+55/‑3)	creC → / → creD	sensory histidine kinase CreC/putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,638,175	0	A	G	100.0%	134.6 / NA	38	intergenic (+55/‑3)	creC/creD	sensory histidine kinase CreC/putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/23);  total (15/23)

ACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAAT  >  NC_000913/4638099‑4638251                                                                             |                                                                             aCTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGGCTAt                                                                          >  2:883066/1‑81 (MQ=255)      aCCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGGCTATGTTg                                                                      >  1:883071/1‑80 (MQ=255)        cGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGGCTATGTTGaaa                                                                   >  2:883079/1‑81 (MQ=255)         gTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGGCTATGTTGAAAt                                                                  >  2:883081/1‑81 (MQ=255)            aCTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGGCTATGTTGAAATccc                                                               <  1:883014/81‑1 (MQ=255)            aCTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGGCTATGTTGAAATcc                                                                <  1:883013/80‑1 (MQ=255)                cacaTAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGGCTATGTTGAAATcccccc                                                            <  2:883015/80‑1 (MQ=255)                 acaTAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTg                                                          >  1:883067/1‑81 (MQ=255)                  caTAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGt                                                         <  1:883016/81‑1 (MQ=255)                    tAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTc                                                       <  1:883017/81‑1 (MQ=255)                     tgCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGt                                                         <  1:883018/77‑1 (MQ=255)                           aaaTTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTCTGGaaaa                                                >  2:883062/1‑81 (MQ=255)                           aaaTTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTCTGGaaa                                                 <  1:883019/80‑1 (MQ=255)                             aTTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTCTGGAAAATg                                              <  1:883021/81‑1 (MQ=255)                             aTTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTCTGGAAAATg                                              <  2:883020/81‑1 (MQ=255)                               tcttcCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTCTGGAAAATGAc                                            >  2:883061/1‑81 (MQ=255)                                cttcCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTCTGGAAAATGAc                                            <  1:883022/80‑1 (MQ=255)                                   cccACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTa                                          <  1:883023/79‑1 (MQ=255)                                       caTAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGt                                    >  2:883074/1‑81 (MQ=255)                                        aTAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGtt                                   <  1:883024/81‑1 (MQ=255)                                         tAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGttt                                  <  1:883025/81‑1 (MQ=255)                                                cGTATCCTGCTGCCATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGc                             <  1:883026/79‑1 (MQ=255)                                                 gTATCCTGCTGCCATTGCAAAGGAGAAGGCTGTGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGt                          <  2:883027/81‑1 (MQ=255)                                                 gTATCCTGCTGCCATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAg                           >  1:883070/1‑80 (MQ=255)                                                  tATCCTGCTGCCATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTa                         >  2:883084/1‑81 (MQ=255)                                                   aTCCTGCTGCCATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTAt                        >  2:883068/1‑81 (MQ=255)                                                      ctgctgCCATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGc                     <  1:883028/81‑1 (MQ=255)                                                       tgctgcCATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCt                    >  1:883082/1‑81 (MQ=255)                                                         ctgcCATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCt                    <  1:883029/79‑1 (MQ=255)                                                            ccATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCtgttgt               >  1:883075/1‑81 (MQ=255)                                                            ccATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCtgttg                <  2:883031/80‑1 (MQ=255)                                                            ccATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCtgttg                <  1:883032/80‑1 (MQ=255)                                                            ccATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCtgttg                <  1:883030/80‑1 (MQ=255)                                                             cATTGCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTgttgtt              <  2:883033/81‑1 (MQ=255)                                                                 gCAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGAtt          >  2:883069/1‑81 (MQ=255)                                                                  cAAAGGAGAAGGCTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTc         <  1:883034/81‑1 (MQ=255)                                                                      ggAGAAGGCTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGa      >  2:883072/1‑80 (MQ=255)                                                                      ggAGAAGGCTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGAt     <  1:883035/81‑1 (MQ=255)                                                                         gaaggCTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAAt  >  1:883076/1‑81 (MQ=255)                                                                             |                                                                             ACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAAT  >  NC_000913/4638099‑4638251

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑