Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	353,323	C→G	S206R (AGC→AGG)	prpE →	propionyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	353,323	0	C	G	100.0%	142.9 / NA	39	S206R (AGC→AGG)	prpE	propionyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (21/18);  total (21/18)

TGCGATAAGTCAGGCGCAGCACCAGCCACGCCATGTTTTGCTGGTGGATCGCGGGCTGGCGAAAATGGCGCGCGTCAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATCAACACATCGGCGCGCGGGTACCGGTGGCGTGGCTGGAATCC  >  NC_000913/353245‑353398                                                                               |                                                                            tGCGATAAGTCAGGCGCAGCACCAGCCACGCCATGTTTTGCTGGTGGATCGCGGGCTGGCGAAAATGGCGCGCGTCAgggg                                                                           >  1:72920/1‑81 (MQ=255)  gCGATAAGTCAGGCGCAGCACCAGCCACGCCATGTTTTGCTGGTGGATCGCGGGCTGGCGAAAATGGCGCGCGTCAggggg                                                                          >  2:72941/1‑81 (MQ=255)  gCGATAAGTCAGGCGCAGCACCAGCCACGCCATGTTTTGCTGGTGGATCGCGGGCTGGCGAAAATGGCGCGCGTCAggggg                                                                          >  2:72910/1‑81 (MQ=255)   cGATAAGTCAGGCGCAGCACCAGCCACGCCATGTTTTGCTGGTGGATCGCGGGCTGGCGAAAATGGCGCGCGTCAggggg                                                                          <  1:72867/80‑1 (MQ=255)       aaGTCAGGCGCAGCACCAGCCACGCCATGTTTTGCTGGTGGATCGCGGGCTGGCGAAAATGGCGCGCGTCAGGGGGCGGGa                                                                     >  2:72926/1‑81 (MQ=255)        aGTCAGGCGCAGCACCAGCCACGCCATGTTTTGCTGGTGGATCGCGGGCTGGCGAAAATGGCGCGCGTCAGGGGGCGGGa                                                                     <  2:72868/80‑1 (MQ=255)         gTCAGGCGCAGCACCAGCCACGCCATGTTTTGCTGGTGGATCGCGGGCTGGCGAAAATGGCGCGCGTCAGGGGGCGGGATg                                                                   >  2:72904/1‑81 (MQ=255)            aGGCGCAGCACCAGCCACGCCATGTTTTGCTGGTGGATCGCGGGCTGGCGAAAATGGCGCGCGTCAGGGGGCGGGATGTCg                                                                <  1:72869/81‑1 (MQ=255)            aGGCGCAGCACCAGCCACGCCATGTTTTGCTGGTGGATCGCGGGCTGGCGAAAATGGCGCGCGTCAGGGGGCGGGATGTCg                                                                <  2:72870/81‑1 (MQ=255)             ggCGCAGCACCAGCCACGCCATGTTTTGCTGGTGGATCGCGGGCTGGCGAAAATGGCGCGCGTCAGGGGGCGGGATGTCGa                                                               >  1:72928/1‑81 (MQ=255)             ggCGCAGCACCAGCCACGCCATGTTTTGCTGGTGGATCGCGGGCTGGCGAAAATGGCGCGCGTCAGGGGGCGGGATGTCGa                                                               >  1:72929/1‑81 (MQ=255)              gcgcTGCCCCAGCCACGCCATGCTTTGCTGGCGGATCGCGGGCTGGCGAAAATGGCGCGTGTCACGGGGCGGGATGTCGAt                                                              >  2:72938/1‑81 (MQ=255)               cgcAGCACCAGCCACGCCATGTTTTGCTGGTGGATCGCGGGCTGGCGAAAATGGCGCGCGTCAGGGGGCGGGATGTCGAtt                                                             <  1:72871/81‑1 (MQ=255)                 cagcaCCAGCCACGCCATGTTTTGCTGGTGGATCGCGGGCTGGCGAAAATGGCGCGCGTCAGGGGGCGGGATGTCGATTTc                                                           <  2:72872/81‑1 (MQ=255)                 cagcaCCAGCCACGCCATGTTTTGCTGGTGGATCGCGGGCTGGCGAAAATGGCGCGCGTCAGGGGGCGGGATGTCGATTTc                                                           <  1:72873/81‑1 (MQ=255)                  agcaCCAGCCACGCCATGTTTTGCTGGTGGATCGCGGGCTGGCGAAAATGGCGCGCGTCAGGGGGCGGGATGTCGATTTcg                                                          <  1:72874/81‑1 (MQ=255)                     aCCAGCCACGCCATGTTTTGCTGGTGGATCGCGGGCTGGCGAAAATGGCGCGCGTCAGGGGGCGGGATGTCGATTTCGcgt                                                       <  1:72875/81‑1 (MQ=255)                     aCCAGCCACGCCATGTTTTGCTGGTGGATCGCGGGCTGGCGAAAATGGCGCGCGTCAGGGGGCGGGATGTCGATTTCGcgt                                                       >  1:72951/1‑81 (MQ=255)                        aGCCACGCCATGTTTTGCTGGTGGATCGCGGGCTGGCGAAAATGGCGCGCGTCAGGGGGCGGGATGTCGATTTCGcgtcgt                                                    >  2:72936/1‑81 (MQ=255)                        aGCCACGCCATGTTTTGCTGGTGGATCGCGGGCTGGCGAAAATGGCGCGCGTCAGGGGGCGGGATGTCGATTTCGcgtcgt                                                    >  2:72922/1‑81 (MQ=255)                          ccACGCCATGTTTTGCTGGTGGATCGCGGGCTGGCGAAAATGGCGCGCGTCAGGGGGCGGGATGTCGATTTCGCGTCGTTg                                                  <  2:72876/81‑1 (MQ=255)                             cGCCATGTTTTGCTGGTGGATCGCGGGCTGGCGAAAATGGCGCGCGTCAGGGGGCGGGATGTCGATTTCGCGTCGTTgcgc                                               <  2:72877/81‑1 (MQ=255)                                   gTTTTGCTGGTGGATCGCGGGCTGGCGAAAATGGCGCGCGTCAGGGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATCaa                                         >  1:72931/1‑81 (MQ=255)                                   gTTTTGCTGGTGGATCGCGGGCTGGCGAAAATGGCGCGCGTCAGGGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATCaa                                         >  1:72925/1‑81 (MQ=255)                                       tGCTGGTGGATCGCGGGCTGGCGAAAATGGCGCGCGTCAGGGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATCAAcaca                                     >  1:72933/1‑81 (MQ=255)                                            gtggATCGCGGGCTGGCGAAAATGGCGCGCGTCAGGGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATCAACACATCGgc                                <  1:72878/81‑1 (MQ=255)                                               gATCGCGGGCTGGCGAAAATGGCGCGCGTCAGGGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATCAACACATCGgcgcg                             <  2:72882/81‑1 (MQ=255)                                               gATCGCGGGCTGGCGAAAATGGCGCGCGTCAGGGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATCAACACATCGgcgc                              <  2:72879/80‑1 (MQ=255)                                               gATCGCGGGCTGGCGAAAATGGCGCGCGTCAGGGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATCAACACATCGgcgc                              <  1:72880/80‑1 (MQ=255)                                               gATCCCGGGCTGGCGAAAATGGCGCGCGTCAGGGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATCAACACATCGgcgc                              <  1:72881/80‑1 (MQ=255)                                                     gggCTGGCGAAAATGGCGCGCGTCAGGGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATCAACACATCGGCGCGCGGGTa                       >  1:72948/1‑81 (MQ=255)                                                     gggCTGGCGAAAATGGCGCGCGTCAGGGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATCAACACATCGGCGCGCGGGTa                       >  1:72946/1‑81 (MQ=255)                                                     gggCTGGCGAAAATGGCGCGCGTCAGGGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATCAACACATCGGCGCGCGGGTa                       >  1:72940/1‑81 (MQ=255)                                                     gggCTGGCGAAAATGGCGCGCGTCAGGGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATCAACACATCGGCGCGCGGGTa                       >  2:72924/1‑81 (MQ=255)                                                        cTGGCGAAAATGGCGCGCGTCAGGGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATCAACACATCGGCGCGCGGGTACCg                    >  1:72953/1‑81 (MQ=255)                                                          ggCGAAAATGGCGCGCGTCAGGGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATCAACACATCGGCGCGCGGGTACCGgt                  >  1:72947/1‑81 (MQ=255)                                                          ggCGAAAATGGCGCGCGTCAGGGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATCAACACATCGGCGCGCGGGTACCGgt                  >  2:72943/1‑81 (MQ=255)                                                          ggCGAAAATGGCGCGCGTCAGGGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATCAACACATCGGCGCGCGGGTACCGgt                  >  2:72958/1‑81 (MQ=255)                                                            cGAAAATGGCGCGCGTCAGGGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATCAACACATCGGCGCGCGGGTACCGgtgg                >  2:72930/1‑81 (MQ=255)                                                              aaaaTGGCGCGCGTCAGGGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATCAACACATCGGCGCGCGGGTACCGgtggcg              >  1:72957/1‑81 (MQ=255)                                                                 aTGGCGCGCGTCAGGGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATCAACACATCGGCGCGCGGGTACCGgtggcgtgg           <  2:72883/81‑1 (MQ=255)                                                                        gcgTCAGGGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATCAACACATCGGCGCGCGGGTACCGGTGGCGTGGCTGGAAt    <  2:72884/81‑1 (MQ=255)                                                                          gTCAGGGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATCAACACATCGGCGCGCGGGTACCGGTGGCGTGGCTGGAATcc  <  1:72885/81‑1 (MQ=255)                                                                               |                                                                            TGCGATAAGTCAGGCGCAGCACCAGCCACGCCATGTTTTGCTGGTGGATCGCGGGCTGGCGAAAATGGCGCGCGTCAGCGGGCGGGATGTCGATTTCGCGTCGTTGCGCCATCAACACATCGGCGCGCGGGTACCGGTGGCGTGGCTGGAATCC  >  NC_000913/353245‑353398

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑