Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	79,341	C→A	G38V (GGT→GTT)	leuD ←	3‑isopropylmalate dehydratase subunit LeuD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	79,341	0	C	A	100.0%	93.7 / NA	27	G38V (GGT→GTT)	leuD	3‑isopropylmalate dehydratase subunit LeuD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (9/18);  total (9/18)

TTCAGCACGAAGTCCGGGTTTGGCTGTTGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAACCCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCATCGGTATCGACATTGGCGGCATCCAGCGG  >  NC_000913/79263‑79414                                                                               |                                                                          ttCAGCACGAAGTCCGAGTTTGGCTGTTGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAacc                                                                         <  2:17204/81‑1 (MQ=255)   cAGCACGAAGTCCGGGTTTGGCTGTTGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCg                                                                        >  2:17242/1‑80 (MQ=255)   cAGCACGAAGTCCGGGTTTGGCTGTTGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCg                                                                        >  1:17247/1‑80 (MQ=255)    aGCACGAAGTCCGGGTTTGGCTGTTGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTa                                                                      >  2:17249/1‑81 (MQ=255)         gAAGTCCGGGTTTGGCTGTTGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGt                                                                 <  1:17206/81‑1 (MQ=255)         gAAGTCCGGGTTTGGCTGTTGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGt                                                                 <  2:17205/81‑1 (MQ=255)          aaGTCCGGGTTTGGCTGTTGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTc                                                                <  2:17207/81‑1 (MQ=255)           aGTCCGGGTTTGGCTGTTGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCa                                                               <  1:17208/81‑1 (MQ=255)             tCCGGGTTTGGCTGTTGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACt                                                             <  2:17209/81‑1 (MQ=255)               cGGGTTTGGCTGTTGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACtt                                                            <  1:17210/80‑1 (MQ=255)                        cTGTTGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCaaaaa                                                  <  1:17211/81‑1 (MQ=255)                           ttGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACt                                                <  1:17212/80‑1 (MQ=255)                              gCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGttt                                            <  2:17213/81‑1 (MQ=255)                               ccTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTc                                           <  2:17214/81‑1 (MQ=255)                                     tCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGgat                                      >  2:17250/1‑80 (MQ=255)                                     tCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGgat                                      <  1:17215/80‑1 (MQ=255)                                       aTCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGgatgat                                   <  2:17216/81‑1 (MQ=255)                                               aaCGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCATTgg                           >  2:17255/1‑81 (MQ=255)                                                aCGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCATCGGt                          <  1:17217/81‑1 (MQ=255)                                                      gTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCATCGGTATCGAc                    <  2:17218/81‑1 (MQ=255)                                                        cGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAATTGTTTCGGGATGATTGCATCGGTATCGACAt                  >  2:17254/1‑81 (MQ=255)                                                         gTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCATCGGTATCGACAtt                 >  1:17266/1‑81 (MQ=255)                                                                aGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCATCGGTATCGACATTGGCGGCa          <  1:17219/81‑1 (MQ=255)                                                                   tGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCATCGGTATCGACATTGGCGGCATcc       <  1:17220/81‑1 (MQ=255)                                                                      gcgcCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCATCGGTATCGACATTGGCGGCATCCAGc    >  1:17265/1‑81 (MQ=255)                                                                      gcgcCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCATCGGTATCGACATTGGCGGCATCCAGc    >  1:17251/1‑81 (MQ=255)                                                                       cgcCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCATCGGTATCGACATTGGCGGCATCCAGCg   <  2:17221/81‑1 (MQ=255)                                                                        gcCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCATCGGTATCGACATTGGCGGCATCCAGCgg  <  1:17222/81‑1 (MQ=255)                                                                               |                                                                          TTCAGCACGAAGTCCGGGTTTGGCTGTTGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAACCCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCATCGGTATCGACATTGGCGGCATCCAGCGG  >  NC_000913/79263‑79414

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑