| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 449,803 | C→A | W280L (TGG→TTG) | cyoB ← | cytochrome bo3 ubiquinol oxidase subunit 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 449,803 | 0 | C | A | 100.0% | 75.2 / NA | 21 | W280L (TGG→TTG) | cyoB | cytochrome bo3 ubiquinol oxidase subunit 1 |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (7/14); total (7/14) | |||||||||||
AAGGTTGCCGCAATTTCGGAGAACACACCGAAAACAGGCAGGATCAGGATGTAAACTTCCGGGTGGCCCCAGGCCCAAATCAGGTTGATGTACATCATCATGTTGCCACCCATATCGTTGGTAAAGAAATGGGTGCCCAGATAGCGATCCAGG > NC_000913/449728‑449880 | aaGGTTGCCGCAATTTCGGAGAACACACCGAAAACAGGCAGGATCAGGATGTAAACTTCCGGGTGGCCCCAGGCCAAAATc < 2:91954/81‑1 (MQ=255) ccGCAATTTCGGAGAACACACCGAAAACAGGCAGGATCAGGATGTAAACTTCCGGGTGGCCCCAGGCCAAAATCAGGTTGa < 2:91955/81‑1 (MQ=255) cAATTTCGGAGAACACACCGAAAACAGGCAGGATCAGGATGTAAACTTCCGGGTGGCCCCAGGCCAAAATCAGGTTGATGt < 1:91956/81‑1 (MQ=255) tCGGAGAACACACCGAAAACAGGCAGGATCAGGATGTAAACTTCCGGGTGGCCCCAGGCCAAAATCAGGTTGATGTAcat > 1:92007/1‑80 (MQ=255) ggAGAACACACCGAAAACAGGCCGGATCAGGATGTAAACTTCCGGGTGGCCCCAGGCCAAAATCAGGTTGATGTAcatcat > 1:91998/1‑81 (MQ=255) ggAGAACACACCGAAAACAGGCAGGATCAGGATGTAAACTTCCGGGTGGCCCCAGGCCAAAATCAGGTTGATGTAcatcat > 1:92003/1‑81 (MQ=255) aaCACACCGAAAACAGGCAGGATCAGGATGTAAACTTCCGGGTGGCCCCAGGCCAAAATCAGGTTGATGTACATCATCATg > 2:91989/1‑81 (MQ=255) acacCGAAAACAGGCAGGATCAGGATGTAAACTTCCGGGTGGCCCCAGGCCAAAATCAGGTTGATGTACATCATCATGTTg < 1:91957/81‑1 (MQ=255) aaaCAGGCAGGATCAGGATGTAAACTTCCGGGTGGCCCCAGGCCAAAATCAGGTTGATGTACATCATCATGTTGCCACTCa > 2:91990/1‑81 (MQ=255) aaCAGGCAGGATCAGGATGTAAACTTCCGGGTGGCCCCAGGCCAAAATCAGGTTGATGTACATCATCATGTTGCCACCCat < 1:91958/81‑1 (MQ=255) aCAGGCAGGATCAGGATGTAAACTTCCGGGTGGCCCCAGGCCAAAATCAGGTTGATGTACATCATCATGTTGCCACCCata > 1:91994/1‑81 (MQ=255) aggcaggATCAGGATGTAAACTTCCGGGTGGCCCCAGGCCAAAATCAGGTTGATGTACATCATCATGTTGCCACCCATATc < 2:91959/81‑1 (MQ=255) ggcaggATCAGGATGTAAACTTCCGGGTGGCCCCAGGCCAAAATCAGGTTGATGTACATCATCATGTTGCCACCCATATCg < 1:91960/81‑1 (MQ=255) tAAACTTCCGGGTGGCCCCAGGCCAAAATCAGGTTGATGTACATCATCATGTTGCCACCCATATCGTTGGTAAAGAAATgg < 2:91962/81‑1 (MQ=255) tAAACTTCCGGGTGGCCCCAGGCCAAAATCAGGTTGATGTACATCATCATGTTGCCACCCATATCGTTGGTAAAGAAATg < 2:91961/80‑1 (MQ=255) cTTCCGGGTGGCCCCAGGCCAAAATCAGGTTGATGTACATCATCATGTTGCCACCCATATCGTTGGTAAAGAAATGGGTGc < 1:91963/81‑1 (MQ=255) ccGGGTGGCCCCAGGCCAAAATCAGGTTGATGTACATCATCATGTTGCCACCCATATCGTTGGTAAAGAAATGGGTGccc < 2:91964/80‑1 (MQ=255) ccGGGTGGCCCCAGGCCAAAATCAGGTTGATGTACATCATCATGTTGCCACCCATATCGTTGGTAAAGAAATGGGTGccc < 2:91965/80‑1 (MQ=255) ccGGGTGGCCCCAGGCCAAAATCAGGTTGATGTACATCATCATGTTGCCACCCATATCGTTGGTAAAGAAATGGGTGccc < 2:91966/80‑1 (MQ=255) ggTGGCCCCAGGCCAAAATCAGGTTGATGTACATCATCATGTTGCCACCCATATCGTTGGTAAAGAAATGGGTGCCCAGa > 1:92001/1‑80 (MQ=255) gCCAAAATCAGGTTGATGTACATCATCATGTTGCCACCCATATCGTTGGTAAAGAAATGGGTGCCCAGATAGCGATCCAgg < 1:91967/81‑1 (MQ=255) | AAGGTTGCCGCAATTTCGGAGAACACACCGAAAACAGGCAGGATCAGGATGTAAACTTCCGGGTGGCCCCAGGCCCAAATCAGGTTGATGTACATCATCATGTTGCCACCCATATCGTTGGTAAAGAAATGGGTGCCCAGATAGCGATCCAGG > NC_000913/449728‑449880 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |