Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	461,034	T→G	I716S (ATC→AGC)	lon →	Lon protease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	461,034	0	T	G	100.0%	116.1 / NA	32	I716S (ATC→AGC)	lon	Lon protease
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (13/19);  total (13/19)

CCTGACCGGTAACCCGGTTCGTGCCGATGTGGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAAT  >  NC_000913/460960‑461107                                                                           |                                                                          ccTGACCGGTAACCCGGTTCGTGCCGATGTGGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGAGCggtg                                                                      <  2:94110/80‑1 (MQ=255)     aCCGGTAACCCGGTTCGTGCCGATGTGGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGAGCGGTGGTTTg                                                                 <  1:94111/81‑1 (MQ=255)       cGGTAACCCGGTTCGTGCCGATGTGGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGAGCGGTGGTTTgaa                                                               <  2:94112/81‑1 (MQ=255)        ggTAACCCGGTTCGTGCCGATGTGGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGAGCGGTGGTTTga                                                                >  1:94157/1‑79 (MQ=255)           aaCCCGGTTCGTGCCGATGTGGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGAGCGGTGGTTTgaaaga                                                            >  2:94158/1‑80 (MQ=255)                     gTGCCGATGTGGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGAGCGGTGGTTTGAAAGAAAAACTCCTgg                                                 <  1:94114/81‑1 (MQ=255)                     gTGCCGATGTGGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGAGCGGTGGTTTGAAAGAAAAACTCCTgg                                                 >  1:94169/1‑81 (MQ=255)                     gTGCCGATGTGGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGAGCGGTGGTTTGAAAGAAAAACTCCTg                                                  <  2:94113/80‑1 (MQ=255)                            tgtgGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGAGCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCa                                          <  2:94115/81‑1 (MQ=255)                             gtgGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGAGCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCAt                                         >  2:94174/1‑81 (MQ=255)                              tgGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGAGCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCAt                                         <  2:94116/80‑1 (MQ=255)                                  aaTGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGAGCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCgcgg                                    <  1:94117/81‑1 (MQ=255)                                    tGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGAGCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCgcggcg                                  <  2:94118/81‑1 (MQ=255)                                         ggTGAGATCACTCTGCGTGGTCAGGTACTGCCGAGCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGAtt                             <  1:94119/81‑1 (MQ=255)                                         ggTGAGATCACTCTGCGTGGTCAGGTACTGCCGAGCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGAt                              >  1:94188/1‑80 (MQ=255)                                          gTGAGATCACTCTGCGTGGTCAGGTACTGCCGAGCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGAtt                             >  2:94167/1‑80 (MQ=255)                                            gagaTCACTCTGCGTGGTCAGGTACTGCCGAGCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTaa                           >  2:94178/1‑80 (MQ=255)                                              gaTCACTCTGCGTGGTCAGGTACTGCCGAGCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAAc                        <  2:94120/81‑1 (MQ=255)                                               aTCACTCTGCGTGGTCAGGTACTGCCGAGCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACa                       <  2:94121/81‑1 (MQ=255)                                                  aCTCTGCGTGGTCAGGTACTGCCGAGCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTg                    >  1:94160/1‑81 (MQ=255)                                                      tGCGTGGTCAGGTACTGCCGAGCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTaa                >  2:94170/1‑81 (MQ=255)                                                       gCGTGGTCAGGTACTGCCGAGCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAAt               >  1:94159/1‑81 (MQ=255)                                                        cGTGGTCAGGTACTGCCGAGCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTa                 <  1:94122/78‑1 (MQ=255)                                                         gTGGTCAGGTACTGCCGAGCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTc             <  1:94123/81‑1 (MQ=255)                                                         gTGGTCAGGTACTGCCGAGCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTc             >  2:94162/1‑81 (MQ=255)                                                             tCAGGTACTGCCGAGCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGtt         >  2:94171/1‑81 (MQ=255)                                                             tCAGGTACTGCCGAGCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGAGATTAAAACAGTGCTAATTCCGtt         <  2:94124/81‑1 (MQ=255)                                                               aGGTACTGCCGAGCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTc        <  2:94125/80‑1 (MQ=255)                                                               aGGTACTGCCGAGCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCg       <  1:94127/81‑1 (MQ=255)                                                               aGGTACTGCCGAGCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCg       <  1:94126/81‑1 (MQ=255)                                                                    cTGCCGAGCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGaaaa   <  2:94128/80‑1 (MQ=255)                                                                     tGCCGAGCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAAt  >  1:94172/1‑80 (MQ=255)                                                                           |                                                                          CCTGACCGGTAACCCGGTTCGTGCCGATGTGGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAAT  >  NC_000913/460960‑461107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑