Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	508,288	A→G	D24G (GAT→GGT)	ybaQ →	putative DNA‑binding transcriptional regulator YbaQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	508,288	0	A	G	100.0%	52.2 / NA	16	D24G (GAT→GGT)	ybaQ	putative DNA‑binding transcriptional regulator YbaQ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (8/8);  total (8/8)

AACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGA  >  NC_000913/508214‑508350                                                                           |                                                               aaCCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGGTTTGa                                                           >  2:103437/1‑80 (MQ=255) aaCCATGAAACAGGCAAAAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGATCGGTTTGaa                                                          <  1:103403/81‑1 (MQ=255)       gAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGGTTTGAAAATCaa                                                    >  1:103425/1‑81 (MQ=255)            aGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGGTTTGAAAATCAATGAGt                                               >  1:103432/1‑81 (MQ=255)             ggCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGGTTTGAAAATCAATGAGtt                                              <  2:103404/81‑1 (MQ=255)                     gAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGGTTTGAAAATCAATGAGTTAGCAGAGt                                      >  1:103434/1‑81 (MQ=255)                          ccGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGGTTTGAAAATCAATGAGTTAGCAGAGTtgctg                                 >  1:103431/1‑81 (MQ=255)                          ccGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGGTTTGAAAATCAATGAGTTAGCAGAGTtgctg                                 >  2:103435/1‑81 (MQ=255)                             acgacACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGGTTTGAAAATCAATGAGTTAGCAGAGTTGCTGCAt                              >  2:103428/1‑81 (MQ=255)                                 cacCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGGTTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTc                          <  2:103405/81‑1 (MQ=255)                                        agaTATTCTTCTCTATGAATATCTGGAACCGCTCGGTTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTaa                   <  1:103406/81‑1 (MQ=255)                                           tatTCTTCTCTATGAATATCTGGAACCGCTCGGTTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAg                <  2:103407/81‑1 (MQ=255)                                                    ctATGAATATCTGGAACCGCTCGGTTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTg        >  2:103441/1‑80 (MQ=255)                                                       tGAATATCTGGAACCGCTCGGTTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGc       <  2:103408/78‑1 (MQ=255)                                                       tGAATATCTGGAACCGCTCGGTTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGc       <  2:103409/78‑1 (MQ=255)                                                         aaTATCTGGAACCGCTCGGTTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGa  <  2:103410/81‑1 (MQ=255)                                                                           |                                                               AACCATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAATCAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGA  >  NC_000913/508214‑508350

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑