Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	538,029	A→G	I133V (ATT→GTT)	ybbW →	putative allantoin transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	538,029	0	A	G	100.0%	101.4 / NA	28	I133V (ATT→GTT)	ybbW	putative allantoin transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/14);  total (14/14)

TTATTAAGGGGCGGAATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGATTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGATTTCACTCTGTTAGGCCTTTCTCTACCGGG  >  NC_000913/537957‑538105                                                                         |                                                                             ttattaAGGGGCGGAATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGGTTCTGAtt                                                                      <  1:109324/81‑1 (MQ=255)      aaGGGGCGGAATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGGTTCTGATTGGCa                                                                  >  2:109356/1‑80 (MQ=255)       aGGGGCGGAATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGGTTCTGATTGGCaaa                                                                <  2:109325/81‑1 (MQ=255)               aaTTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGGTTCTGATTGGCAAAATCTGGcc                                                        >  1:109355/1‑81 (MQ=255)                  tGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGGTTCTGATTGGCAAAATCTGGCCgg                                                      >  2:109352/1‑80 (MQ=255)                   gccgccATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGGTTCTGATTGGCAAAATCTGGCCGGGa                                                    >  1:109357/1‑81 (MQ=255)                    ccgccATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGGTTCTGATTGGCAAAATCTGGCCGGGAt                                                   >  2:109361/1‑81 (MQ=255)                     cgccATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGGTTCTGATTGGCAAAATCTGGCCGGGAtt                                                  >  1:109364/1‑81 (MQ=255)                       ccATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGGTTCTGATTGGCAAAATCTGGCCGGGAtttt                                                >  1:109373/1‑81 (MQ=255)                         atcatGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGGTTCTGATTGGCAAAATCTGGCCGGGAttttt                                               >  2:109358/1‑80 (MQ=255)                            atGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGGTTCTGATTGGCAAAATCTGGCCGGGATTTTTAAct                                           <  1:109326/81‑1 (MQ=255)                                 gtttggtttgCAATGTTACGCGGGGTCACTGGCCTGCTTGGTTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCg                                       <  1:109327/80‑1 (MQ=255)                                      gtttgCAATGTTACGCGGGGTCACTGGCCTGCTTGGTTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCggtggt                                  <  2:109328/80‑1 (MQ=255)                                      gtttgCAATGTTACGCGGGGTCACTGGCCTGCTTGGTTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGgtggtg                                 <  2:109329/81‑1 (MQ=255)                                       tttgCAATGTTACGCGGGGTCACTGGCCTGCTTGGTTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGa                                >  1:109360/1‑81 (MQ=255)                                         tgCAATGTTACGCGGGGTCACTGGCCTGCTTGGTTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGAt                               <  1:109330/80‑1 (MQ=255)                                             aTGTTACGCGGGGTCACTGGCCTGCTTGGTTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGATTTCAc                          >  1:109371/1‑81 (MQ=255)                                               gTTACGCGGGGTCACTGGCCTGCTTGGTTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGATTTCActc                        >  2:109372/1‑81 (MQ=255)                                                 tACGCGGGGTCACTGGCCTGCTTGGTTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGATTTCACTCTg                      >  2:109365/1‑81 (MQ=255)                                                  aCGCGGGGTCACTGGCCTGCTTGGTTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGATTTCACTCTg                      <  1:109331/80‑1 (MQ=255)                                                   cgcgGGGTCACTGGCCTGCTTGGTTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGATTTCACTCTGt                     <  1:109332/80‑1 (MQ=255)                                                         gTCACTGGCCTGCTTGGTTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGATTTCACTCTGTTAGGCCt              >  2:109362/1‑81 (MQ=255)                                                            aCTGGCCTGCTTGGTTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGATTTCACTCTGTTAGGCCTTtc           <  1:109333/81‑1 (MQ=255)                                                                gCCTGCTTGGTTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGATTTCACTCTGTTAGGCCTTtctc         <  2:109334/79‑1 (MQ=255)                                                                gCCTGCTTGGTTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGATTTCACTCTGTTAGGCCTTTctct        <  1:109335/80‑1 (MQ=255)                                                                   tGCTTGGTTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGATTTCACTCTGTTAGACCTTTCTCTACCg    >  1:109363/1‑81 (MQ=255)                                                                     cTTGGTTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGATTTCACTCTGTTAGGCCTTTCTCTACCggg  <  2:109337/81‑1 (MQ=255)                                                                     cTTGGTTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGATTTCACTCTGTTAGGCCTTTCTCTACCgg   <  1:109336/80‑1 (MQ=255)                                                                         |                                                                             TTATTAAGGGGCGGAATTGCCGCCATCATGTGGTTTGGTTTGCAATGTTACGCGGGGTCACTGGCCTGCTTGATTCTGATTGGCAAAATCTGGCCGGGATTTTTAACTCTCGGTGGTGATTTCACTCTGTTAGGCCTTTCTCTACCGGG  >  NC_000913/537957‑538105

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑