Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	91,323	G→A	E98K (GAA→AAA)	ftsL →	cell division protein FtsL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	91,323	0	G	A	100.0%	164.2 / NA	43	E98K (GAA→AAA)	ftsL	cell division protein FtsL
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/24);  total (19/24)

GGAGCGAGATGCTTTAGACATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGGAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGTTGATCCGTCACAAGAAAATATCGTAGTGCAAAAATAA  >  NC_000913/91247‑91397                                                                             |                                                                           ggAGCGAGATGCTTTAGACATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGAAAAg                                                                        >  1:19690/1‑81 (MQ=255) ggAGCGAGATGCTTTAGACATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGAAAAg                                                                        >  1:19689/1‑81 (MQ=255)   aGCGAGATGCTTTAGACATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGAAAAGGa                                                                      <  1:19638/81‑1 (MQ=255)    gCGAGATGCTTTAGACATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGAAAAGGAt                                                                     >  1:19698/1‑81 (MQ=255)        gaTGCTTTAGACATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGAAAAGGATCGc                                                                  >  1:19685/1‑80 (MQ=255)          tGCTTTAGACATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGAAAAGGATCGCCAc                                                               <  2:19639/81‑1 (MQ=255)            cTTTAGACATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGAAAAGGATCGCCACg                                                              <  1:19640/80‑1 (MQ=255)               tAGACATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGAAAAGGATCGCCACGGaa                                                           <  2:19643/80‑1 (MQ=255)               tAGACATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGAAAAGGATCGCCACGGaa                                                           <  2:19642/80‑1 (MQ=255)               tAGACATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGAAAAGGATCGCCACGGa                                                            <  2:19641/79‑1 (MQ=255)                  aCATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGAAAAGGATCGCCACGGAAAAGc                                                       <  1:19644/81‑1 (MQ=255)                   caatGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGAAAAGGATCGCCACGGAAAAGCt                                                      >  1:19684/4‑81 (MQ=255)                    aTTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGAAAAGGATCGCCACGGAAAAGCTg                                                     >  1:19701/1‑81 (MQ=255)                        aaTGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGAAAAGGATCGCCACGGAAAAGCTGCaaa                                                 >  2:19706/1‑81 (MQ=255)                              gcAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGAAAAGGATCGCCACGGAAAAGCTGCAAATgcagc                                           <  1:19645/81‑1 (MQ=255)                                aaCCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGAAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCAt                                         >  1:19694/1‑81 (MQ=255)                                aaCCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGAAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCAt                                         <  2:19647/81‑1 (MQ=255)                                aaCCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGAAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCAt                                         <  1:19646/81‑1 (MQ=255)                                aaCCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGAAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCAt                                         <  1:19648/81‑1 (MQ=255)                                 aCCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGAAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATg                                        <  2:19649/81‑1 (MQ=255)                                  ccTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGAAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGt                                       >  1:19696/1‑81 (MQ=255)                                   cTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGAAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGtt                                      >  1:19691/1‑81 (MQ=255)                                     gATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGAAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGTTGa                                    >  2:19699/1‑81 (MQ=255)                                      aTCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGAAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGTTg                                     <  1:19650/79‑1 (MQ=255)                                         cTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGAAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGTTGATcc                                 <  2:19651/80‑1 (MQ=255)                                         cTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGAAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGTTGATCCg                                <  1:19652/81‑1 (MQ=255)                                           tGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGAAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGTTGATCCGt                               >  2:19711/1‑80 (MQ=255)                                              agagAATGCGCTCGGCGACCATAGCCGGGTGAAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCTTGTTGATCCGTcaca                           <  1:19653/81‑1 (MQ=255)                                                agaATGCGCTCGGCGACCATAGCCGGGTGAAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGTTGATCCGTCACAAg                         <  2:19654/81‑1 (MQ=255)                                                  aaTGCGCTCGGCGACCATAGCCGGGTGAAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGTTGATCCGTCACAAGa                        >  1:19702/1‑80 (MQ=255)                                                   aTGCGCTCGGCGACCATAGCCGGGTGAAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGTTGATCCGTCACAAGaaa                      <  1:19655/81‑1 (MQ=255)                                                       gcTCGGCGACCATAGCCGGGTGAAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGTTGATCCGTCACAAGAAAatat                  >  1:19693/1‑81 (MQ=255)                                                        cTCGGCGACCATAGCCGGGTGAAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGTTGATCCGTCACAAGAAAATATc                 >  1:19721/1‑81 (MQ=255)                                                            gCGACCATAGCCGGGTGAAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGTTGATCCGTCACAAGAAAATATCGTAg             >  2:19700/1‑81 (MQ=255)                                                            gCGACCATAGCCGGGTGAAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGTTGATCCGTCACAAGAAAATATCGTAg             <  1:19656/81‑1 (MQ=255)                                                            gCGACCATAGCCGGGTGAAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGTTGATCCGTCACAAGAAAATATCGTAg             <  1:19657/81‑1 (MQ=255)                                                            gCGACCATAGCCGGGTGAAAAGGACCACCACGGAAAAGCCGCAAATGCAGCATGTTGATCCGTCACAAGTAAATATCGTAg             >  2:19697/1‑81 (MQ=255)                                                              gACCATAGCCGGGTGAAAAGGATCGCCACGGGAAAGCTGCAAATGCAGCATGTTGATCCGTCACAAGAAAATATCGTAGTg           <  2:19658/81‑1 (MQ=255)                                                              gACCATAGCCGGGTGAAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGTTGATCCGTCACAAGAAAATATCGTAGTg           >  1:19712/1‑81 (MQ=255)                                                                 cATAGCCGGGTGAAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGTTGATCCGTCACAAGAAAATATCGTAGTGCaa        <  2:19659/81‑1 (MQ=255)                                                                     gCCGGGTGAAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGTTGATCCGTCACAAGAAAATATCGTAGTGCAAAAAt    <  2:19660/81‑1 (MQ=255)                                                                       cgagTGAAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGTTGATCCGTCACAAGAAAATATCGTAGTGCAAAAATaa  <  2:19661/78‑1 (MQ=255)                                                                       cGGGTGAAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGTTGATCCGTCACAAGAAAATATCGTAGTGCAAAAAt    >  1:19704/1‑79 (MQ=255)                                                                             |                                                                           GGAGCGAGATGCTTTAGACATTGAATGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGGAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGTTGATCCGTCACAAGAAAATATCGTAGTGCAAAAATAA  >  NC_000913/91247‑91397

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑