Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	698,541	G→T	R213S (CGT→AGT)	asnB ←	asparagine synthetase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	698,541	0	G	T	100.0%	126.6 / NA	34	R213S (CGT→AGT)	asnB	asparagine synthetase B
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (17/17);  total (17/17)

GACCACCAGAAAGCAGCACACCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTT  >  NC_000913/698466‑698618                                                                            |                                                                              gACCACCAGAAAGCAGCACACCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCt                                                                          <  1:137947/81‑1 (MQ=255)  accaccAGAAAGCAGCACACCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTc                                                                         <  1:137948/81‑1 (MQ=255)  accaccAGAAAGCAGCACACCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTc                                                                         >  1:137991/1‑81 (MQ=255)   ccaccaGAAAGCAGCACACCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCg                                                                        >  2:137980/1‑81 (MQ=255)    caccaGAAAGCAGCACACCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGt                                                                       >  1:137994/1‑81 (MQ=255)      ccaGAAAGCAGCACACCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGttt                                                                     >  1:137987/1‑81 (MQ=255)        aGAAAGCAGCACACCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGttttt                                                                   >  1:137998/1‑81 (MQ=255)           aaGCAGCACACCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTc                                                                >  2:137984/1‑81 (MQ=255)                  acacCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACg                                                         >  2:138009/1‑81 (MQ=255)                    acCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGt                                                        >  2:137985/1‑80 (MQ=255)                       gTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATc                                                    >  1:138000/1‑81 (MQ=255)                           ggCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTc                                                <  2:137949/81‑1 (MQ=255)                              acatcagacatcagATGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCAcc                                             <  2:137950/81‑1 (MQ=255)                                   agacatcagaTGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATc                                        <  1:137951/81‑1 (MQ=255)                                    gacatcagaTGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCGTCg                                       <  2:137952/81‑1 (MQ=255)                                    gacatcagaTGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCg                                       >  2:138007/1‑81 (MQ=255)                                    gacatcagaTGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCg                                       <  1:137953/81‑1 (MQ=255)                                     acatcagaTGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGt                                      <  1:137954/81‑1 (MQ=255)                                          agaTGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCg                                 <  1:137955/81‑1 (MQ=255)                                               gCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAAcc                             <  1:137956/80‑1 (MQ=255)                                                   ttAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTTACCGCATCGTAGTCGAACCAGTc                         <  2:137957/80‑1 (MQ=255)                                                     aaCTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTcgc                       <  2:137958/80‑1 (MQ=255)                                                      aCTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTcgcg                      <  2:137959/80‑1 (MQ=255)                                                      aCTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCga                     >  2:138003/1‑81 (MQ=255)                                                       cTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCgat                    <  1:137960/81‑1 (MQ=255)                                                         gAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCgatga                  >  2:138004/1‑81 (MQ=255)                                                          aaTCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCgatga                  >  1:138001/1‑80 (MQ=255)                                                            tCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAg               >  2:137997/1‑81 (MQ=255)                                                              ttCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGt              >  2:138002/1‑80 (MQ=255)                                                                ccAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGt              <  1:137961/78‑1 (MQ=255)                                                                 cAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCTAACCAGTCGCGATGATAGTAAg           >  2:137996/1‑80 (MQ=255)                                                                   gTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAAc        <  2:137962/81‑1 (MQ=255)                                                                       cTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGAt    <  2:137963/81‑1 (MQ=255)                                                                         gACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGAttt  >  1:138006/1‑81 (MQ=255)                                                                            |                                                                              GACCACCAGAAAGCAGCACACCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTT  >  NC_000913/698466‑698618

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑