Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	797,736	T→C	E232E (GAA→GAG)	ybhA ←	pyridoxal phosphate/fructose‑1,6‑bisphosphate phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	797,736	0	T	C	100.0%	107.0 / NA	30	E232E (GAA→GAG)	ybhA	pyridoxal phosphate/fructose‑1,6‑bisphosphate phosphatase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/13);  total (17/13)

TATCACCAATCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCA  >  NC_000913/797660‑797813                                                                             |                                                                              tATCACCAATCACAATGTTCGCGCGCGCTTTCACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTc                                                                             >  2:155399/1‑79 (MQ=255)    cACCAATCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCa                                                                        >  2:155392/1‑81 (MQ=255)     aCCAATCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCAt                                                                       <  2:155357/81‑1 (MQ=255)          tCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGa                                                                  <  1:155358/81‑1 (MQ=255)               aTGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCa                                                             >  2:155393/1‑81 (MQ=255)                    ggCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCATTa                                                          >  2:155406/1‑79 (MQ=255)                        cgcgcTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCATTAAAGTTa                                                    <  1:155359/81‑1 (MQ=255)                        cgcgcTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCATTAAAGTTa                                                    >  1:155414/1‑81 (MQ=255)                               ttACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCATTAAAGTTATCGCCg                                              >  2:155397/1‑80 (MQ=255)                               ttACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCATTAAAGTTATCGCCg                                              >  2:155394/1‑80 (MQ=255)                                  ccGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCATTAAAGTTATCGCCGAAt                                           <  2:155360/80‑1 (MQ=255)                                  ccGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCATTAAAGTTATCGCCGAAt                                           <  2:155361/80‑1 (MQ=255)                                   cgcgTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGc                                         <  2:155362/81‑1 (MQ=255)                                     cgTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCa                                       <  1:155364/81‑1 (MQ=255)                                     cgTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCa                                       <  2:155363/81‑1 (MQ=255)                                      gTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCac                                      <  1:155365/81‑1 (MQ=255)                                         aTCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCacgac                                   <  1:155366/81‑1 (MQ=255)                                           cGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGt                                 >  2:155398/1‑81 (MQ=255)                                              cGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGtttt                              <  1:155367/81‑1 (MQ=255)                                               gTTGCCCATCGCCACGCCTGTACCAGCGTCCTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTc                             >  1:155402/1‑81 (MQ=255)                                                  gCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCAt                          >  2:155404/1‑81 (MQ=255)                                                   cccATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATc                         <  2:155368/81‑1 (MQ=255)                                                    ccATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATc                         >  1:155395/1‑80 (MQ=255)                                                         gCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCaa                   >  1:155405/1‑81 (MQ=255)                                                          ccACGCCTGTACCAGCGGCCTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAAc                  >  1:155415/1‑81 (MQ=255)                                                          ccACGCCTGTACCAGCGGCCTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAAc                  <  1:155369/81‑1 (MQ=255)                                                                 tGTACCAGCGGCCTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTgcgc           >  2:155409/1‑81 (MQ=255)                                                                       aGCGGCCTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAAc     >  1:155408/1‑81 (MQ=255)                                                                       aGCGGCCTCCAGCATACTGATATCATTAAAGTTATCGCCGAATCCCTCGACGTTTTCCATCGCCCAACCTTGCGCCTCAAc     >  1:155407/1‑81 (MQ=255)                                                                          ggCCTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCa  >  2:155410/1‑81 (MQ=255)                                                                             |                                                                              TATCACCAATCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCA  >  NC_000913/797660‑797813

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑