Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	814,742	G→C	G406A (GGC→GCC)	uvrB →	excision nuclease subunit B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	814,742	0	G	C	100.0%	155.8 / NA	44	G406A (GGC→GCC)	uvrB	excision nuclease subunit B
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (21/23);  total (21/23)

GAGTTCGAAGCATTAGCGCCGCAAACCATCTATGTTTCGGCGACGCCGGGTAATTACGAGCTGGAAAAATCCGGCGGCGATGTGGTGGATCAGGTGGTGCGTCCAACCGGATTGCTTGACCCGATTATCGAAGTGCGGCCGGTGGCGACAC  >  NC_000913/814666‑814816                                                                             |                                                                           gagTTCGAAGCATTAGCGCCGCAAACCATCTATGTTTCGGCGACGCCGGGTAATTACGAGCTGGAAAAATCCGGCGCCGAt                                                                        <  1:158320/81‑1 (MQ=255)     tCGAAGCATTAGCGCCGCAAACCATCTATGTTTCGGCGACGCCGGGTAATTACGAGCTGGAAAAATCCGGCGCCGATgtgg                                                                    >  2:158360/1‑81 (MQ=255)      cGAAGCATTAGCGCCGCAAACCATCTATGTTTCGGCGACGCCGGGTAATTACGAGCTGGAAAAATCCGGCGCCGATgtgg                                                                    <  1:158321/80‑1 (MQ=255)       gAAGCATTAGCGCCGCAAACCATCTATGTTTCGGCGACGCCGGGTAATTACGAGCTGGAAAAATCCGGCGCCGATgtggtg                                                                  <  2:158322/81‑1 (MQ=255)        aaGCATTAGCGCCGCAAACCATCTATGTTTCGGCGACGCCGGGTAATTACGAGCTGGAAAAATCCGGCGCCGATGtggtgg                                                                 <  1:158323/81‑1 (MQ=255)            aTTAGCGCCGCAAACCATCTATGTTTCGGCGACGCCGGGTAATTACGAGCTGGGAAAATCCGGCGCCGATGTGGTGGAt                                                               >  2:158367/1‑79 (MQ=255)            aTTAGCGCCGCAAACCATCTATGTTTCGGCGACGCCGGGTAATTACGAGCTGGAAAAATCCGGCGCCGATGTGGTGGATCa                                                             <  2:158324/81‑1 (MQ=255)              tAGCGCCGCAAACCATCTATGTTTCGGCGACGCCGGGTAATTACGAGCTGGAAAAATCCGGCGCCGATGTGGTGGATCAgg                                                           >  2:158366/1‑81 (MQ=255)               aGCGCCGCAAACCATCTATGTTTCGGCGACGCCGGGTAATTACGAGCTGGAAAAATCCGGCGCCGATGTGGTGGATCAggt                                                          >  1:158370/1‑81 (MQ=255)                gcgcCGCAAACCATCTATGTTTCGGCGACGCCGGGTAATTACGAGCTGGAAAAATCCGGCGCCGATGTGGTGGATCAggtg                                                         >  1:158364/1‑81 (MQ=255)                  gccgcAAACCATCTATGTTTCGGCGACGCCGGGTAATTACGAGCTGGAAAAATCCGGCGCCGATGTGGTGGATCAggtggt                                                       >  1:158376/1‑81 (MQ=255)                  gccgcAAACCATCTATGTTTCGGCGACGCCGGGTAATTACGAGCTGGAAAAATCCAGCGCCGATGTGGTGGATCAggtggt                                                       <  2:158325/81‑1 (MQ=255)                   ccgcAAACCATCTATGTTTCGGCGACGCCGGGTAATTACGAGCTGGAAAAATCCGGCGCCGATGTGGTGGATCAGgtggtg                                                      >  1:158374/1‑81 (MQ=255)                    cgcAAACCATCTATGTTTCGGCGACGCCGGGTAATTACGAGCTGGAAAAATCCGGCGCCGATGTGGTGGATCAGGTGGTGc                                                     <  2:158326/81‑1 (MQ=255)                      cAAACCATCTATGTTTCGGCGACGCCGGGTAATTACGAGCTGGAAAAATCCGGCGCCGATGTGGTGGATCAGGTGGTGCGt                                                   <  2:158327/81‑1 (MQ=255)                       aaaCCATCTATGTTTCGGCGACGCCGGGTAATTACGAGCTGGAAAAATCCGGCGCCGATGTGGTGGATCAGGTGGTGCGTc                                                  <  1:158328/81‑1 (MQ=255)                           cATCTATGTTTCGGCGACGCCGGGTAATTACGAGCTGGAAAAATCCGGCGCCGATGTGGTGGATCAGGTGGTGCGTCCAAc                                              <  1:158329/81‑1 (MQ=255)                            aTCTATGTTTCGGCGACGCCGGGTAATTACGAGCTGGAAAAATCCGGCGCCGATGTGGTGGATCAGGTGGTGCGTCCAAcc                                             >  2:158362/1‑81 (MQ=255)                                aTGTTTCGGCGACGCCGGGTAATTACGAGCTGGAAAAATCCGGCGCCGATGTGGTGGATCAGGTGGTGCGTCCAACCGGAt                                         >  2:158363/1‑81 (MQ=255)                                     tCGGCGACGCCGGGTAATTACGAGCTGGAAAAATCCGGCGCCGATGTGGTGGATCAGGTGGTGCGTCCAACCGGATTGCt                                     <  2:158330/80‑1 (MQ=255)                                      cGGCGACGCCGGGTAATTACGAGCTGGAAAAATCCGGCGCCGATGTGGTGGATCAGGTGGTGCGTCCAACCGGATTGCtt                                    >  1:158385/1‑80 (MQ=255)                                       ggCGACGCCGGGTAATTACGAGCTGGAAAAATCCGGCGCCGATGTGGTGGATCAGGTGGTGCGTCCAACCGGATTGCTTg                                   <  1:158332/80‑1 (MQ=255)                                       ggCGACGCCGGGTAATTACGAGCTGGAAAAATCCGGCGCCGATGTGGTGGATCAGGTGGTGCGTCCAACCGGATTGCTTg                                   <  2:158331/80‑1 (MQ=255)                                       ggCGACGCCGGGTAATTACGAGCTGGAAAAATCCGGCGCCGATGTGGTGGATCAGGTGGTGCGTCCAACCGGATTGCTTGa                                  >  2:158375/1‑81 (MQ=255)                                        gCGACGCCGGGTAATTACGATCTGGAAAAATCCGGCGCCGATGTGGTGGATCAGGTGGTGCGTCCAACCGGATTGCTTGAc                                 <  2:158333/81‑1 (MQ=255)                                         cGACGCCGGGTAATTACGAGCTGGAAAAATCCGGCGCCGATGTGGTGGATCAGGTGGTGCGTCCAACCGGATTGCTTGAcc                                >  2:158379/1‑81 (MQ=255)                                          gACGCCGGGTAATTACGAGCTGGAAAAATCCGGCGCCGATGTGGTGGATCAGGTGGTGCGTCCAACCGGATTGCTTGAccc                               >  1:158369/1‑81 (MQ=255)                                               cGGGTAATTACGAGCTGGAAAAATCCGGCGCCGATGTGGTGGATCAGGTGGTGCGTCCAACCGGATTGCTTGACCCGATTa                          >  2:158387/1‑81 (MQ=255)                                                gggTAATTACGAGCTGGAAAAATCCGGCGCCGATGTGGTGGATCAGGTGGTGCGTCCAACCGGATTGCTTGACCCGATTAt                         <  2:158334/81‑1 (MQ=255)                                                 ggTAATTACGAGCTGGAAAAATCCGGCGCCGATGTGGTGGATCAGGTGGTGCGTCCAACCGGATTGCTTGACCCGATTATc                        <  2:158335/81‑1 (MQ=255)                                                  gTAATTACGAGCTGGAAAAATCCGGCGCCGATGTGGTGGATCAGGTGGTGCGTCCAACCGGATTGCTTGACCCGATTATc                        >  1:158365/1‑80 (MQ=255)                                                     agtACGAGCTGGAAAAATCCGGCGCCGATGTGGTGGATCAGGGGGTGCGTCCAACCGGATTGCTTGACCCGATTATCGAAg                    <  2:158336/79‑1 (MQ=255)                                                     aTTACGAGCTGGAAAAATCCGGCGCCGATGTGGTGGATCAGGTGGTGCGTCCAACCGGATTGCTTGACCCGATTATCGAAg                    >  1:158377/1‑81 (MQ=255)                                                         cGAGCTGGAAAAATCCGGCGCCGATGTGGTGGATCAGGTGGTGCGTCCAACCGGATTGCTTGACCCGATTATCGAAGTGCg                >  2:158380/1‑81 (MQ=255)                                                          gAGCTGGAAAAATCCGGCGCCGATGTGGTGGATCAGGTGGTGCGTCCAACCGGATTGCTTGACCCGATTATCGAAGTGCgg               <  2:158338/81‑1 (MQ=255)                                                          gAGCTGGAAAAATCCGGCGCCGATGTGGTGGATCAGGTGGTGCGTCCAACCGGATTGCTTGACCCGATTATCGAAGTGCgg               >  2:158373/1‑81 (MQ=255)                                                          gAGCTGGAAAAATCCGGCGCCGATGTGGTGGATCAGGTGGTGCGTCCAACCGGATTGCTTGACCCGATTATCGAAGTGCgg               <  1:158337/81‑1 (MQ=255)                                                               ggAAAAATCCGGCGCCGATGTGGTGGATCAGGTGGTGCGTCCAACCGGATTGCTTGACCCGATTATCGAAGTGCGGCCGGt          >  2:158378/1‑81 (MQ=255)                                                                   aaaTCCGGCGCCGATGTGGTGGATCAGGTGGTGCGTCCAACCGGATTGCTTGACCCGATTATCGAAGTGCGGCCGGTGGCg      <  2:158339/81‑1 (MQ=255)                                                                    aaTCCGGCGCCGATGTGGTGGATCAGGTGGTGCGTCCAACCGGATTGCTTGACCCGATTATCGAAGTGCGGCCGGTGGCGa     >  2:158390/1‑81 (MQ=255)                                                                     aTCCGGCGCCGATGTGGTGGATCAGGTGGTGCGTCCAACCGGATTGCTTGACCCGATTATCGAAGTGCGGCCGGTGGCGac    <  2:158341/81‑1 (MQ=255)                                                                     aTCCGGCGCCGATGTGGTGGATCAGGTGGTGCGTCCAACCGGATTGCTTGACCCGATTATCGAAGTGCGGCCGGTGGCGac    <  2:158340/81‑1 (MQ=255)                                                                      tCCGGCGCCGATGTGGTGGATCAGGTGGTGCGTCCAACCGGATTGCTTGACCCGATTATCGAAGTGCGGCCGGTGGCGaca   <  2:158342/81‑1 (MQ=255)                                                                       ccGGCGCCGATGTGGTGGATCAGGTGGTGCGTCCAACCGGATTGCTTGACCCGATTATCGAAGTGCGGCCGGTGGCGaca   >  2:158384/1‑80 (MQ=255)                                                                        cggcgcCGATGTGGTGGATCAGGTGGTGCGTCCAACCGGATTGCTTGACCCGATTATCGAAGTGCGGCCGGTGGCGacac  <  1:158343/80‑1 (MQ=255)                                                                        cggcgcCGATGTGGTGGATCAGGTGGTGCGTCCAACCGGATTGCTTGACCCGATTATCGAAGTGCGGCCGGTGGCGacac  <  2:158344/80‑1 (MQ=255)                                                                        cggcgcCGATGTGGTGGATCAGGTGGTGCGTCCAACCGGATTGCTTGACCCGATTATCGAAGTGCGGCCGGTGGCGaca   >  1:158399/1‑79 (MQ=255)                                                                        cggcgcCGATGTGGTGGATCAGGTGGTGCGTCCAACCGGATTGCTTGACCCGATTATCGAAGTGCGGCCGGTGGCGaca   >  2:158396/1‑79 (MQ=255)                                                                             |                                                                           GAGTTCGAAGCATTAGCGCCGCAAACCATCTATGTTTCGGCGACGCCGGGTAATTACGAGCTGGAAAAATCCGGCGGCGATGTGGTGGATCAGGTGGTGCGTCCAACCGGATTGCTTGACCCGATTATCGAAGTGCGGCCGGTGGCGACAC  >  NC_000913/814666‑814816

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑