Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	909,605	A→T	L482Q (CTA→CAA)	poxB ←	pyruvate oxidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	909,605	0	A	T	100.0%	130.4 / NA	34	L482Q (CTA→CAA)	poxB	pyruvate oxidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (14/20);  total (14/20)

CAACTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAA  >  NC_000913/909528‑909678                                                                              |                                                                          caACTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTgttgtt                                                                        >  1:174194/1‑81 (MQ=255)       cAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTTGTTCGGt                                                                    <  2:174151/79‑1 (MQ=255)       cAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTTGTTCGGTGc                                                                  >  1:174193/1‑81 (MQ=255)        aGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTTGTTCGGTGcc                                                                 <  2:174152/81‑1 (MQ=255)        aGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTTGTTCGGTGc                                                                  <  1:174153/80‑1 (MQ=255)                 ttCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTTGTTCGGTGCCgtcagtca                                                         <  2:174154/80‑1 (MQ=255)                  tCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTTGTTCGGTGCCGTCAGTCaa                                                        <  2:174155/80‑1 (MQ=255)                   cTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTTGTTCGGTGCCGTCAGTCaaa                                                       <  1:174156/80‑1 (MQ=255)                   cTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTTGTTCGGTGCCGTCAGTCaaa                                                       >  2:174173/1‑80 (MQ=255)                      cacGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTTGTTCGGTGCCGTCAGTCAAAt                                                      <  2:174157/78‑1 (MQ=255)                          gATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTTGTTCGGTGCCGTCAGTCAAATAGccac                                                >  1:174190/1‑80 (MQ=255)                           aTACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTTGTTCGGTGCCGTCAGTCAAATAGccacca                                              <  1:174159/81‑1 (MQ=255)                           aTACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTTGTTCGGTGCCGTCAGTCAAATAGccacca                                              <  2:174158/81‑1 (MQ=255)                                cGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTTGTTCGGTGCCGTCAGTCAAATAGCCACCAGCttt                                         <  1:174160/81‑1 (MQ=255)                                   aaTGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTTGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCAt                                      >  2:174208/1‑81 (MQ=255)                                      gCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTTGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCAtctc                                   >  1:174202/1‑81 (MQ=255)                                         gCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTTGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCAt                                >  2:174211/1‑81 (MQ=255)                                          cACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTTGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATc                               <  2:174162/81‑1 (MQ=255)                                          cACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTTGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATc                               <  1:174161/81‑1 (MQ=255)                                           aCGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTTGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCg                              >  1:174195/1‑81 (MQ=255)                                             gCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTTGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGc                             >  2:174201/1‑80 (MQ=255)                                               ttCGGCAATGCGGGCAAAGTTTGTGTCGTGTTGTTCGGTGCCGTCAGTCTAATAGCCACCAGCTTTCATCTCCATCGCca                           >  2:174204/1‑80 (MQ=255)                                                 cGGCAATGCGGGCAAAGTTTGTGTCGTGTTGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCcaca                         >  1:174207/1‑80 (MQ=255)                                                 cGGCAATGCGGGCAAAGTTTGTGTCGTGTTGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCcac                          <  1:174163/79‑1 (MQ=255)                                                      aTGCGGGCAAAGTTTGTGTCGTGTTGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGccc                   <  1:174165/81‑1 (MQ=255)                                                      aTGCGGGCAAAGTTTGTGTCGTGTTGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGccc                   <  2:174164/81‑1 (MQ=255)                                                        gCGGGCAAAGTTTGTGTCGTGTTGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAg                 >  2:174203/1‑81 (MQ=255)                                                           ggCAAAGTTTGTGTCGTGTTGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCAc              >  1:174219/1‑81 (MQ=255)                                                           ggCAAAGTTTGTGTCGTGTTGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCAc              <  2:174166/81‑1 (MQ=255)                                                             cAAAGTTTGTGTCGTGTTGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGc            >  2:174217/1‑81 (MQ=255)                                                              aaaGTTTGTGTCGTGTTGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCt           >  1:174214/1‑81 (MQ=255)                                                                 gTTTGTGTCGTGTTGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCtgtt        <  1:174167/81‑1 (MQ=255)                                                                  tttGTGTCGTGTTGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCtgttg       <  1:174168/81‑1 (MQ=255)                                                                    tgtgTCGTGTTGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCtgttgt      <  1:174169/80‑1 (MQ=255)                                                                        tCGTGTTGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTaaa  <  1:174170/80‑1 (MQ=255)                                                                              |                                                                          CAACTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAA  >  NC_000913/909528‑909678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑