Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	914,159	T→C	E233E (GAA→GAG)	lysO ←	L‑lysine exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	914,159	0	T	C	100.0%	62.0 / NA	18	E233E (GAA→GAG)	lysO	L‑lysine exporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (6/12);  total (6/12)

GCACCGCATAAGCCCAGTGCAGTAGAGCGGCTGCGGCGAATCAGCCCAGGGATCAACATAATAGCAATCAGTTCACGGGCCAGATCATTAAAAAACGCCGCGCTCCCGATTACCGGACCAAAAGATTCGGTCAATAAAATACCGGA  >  NC_000913/914088‑914233                                                                        |                                                                           gCACCGCATAAGCCCAGTGCAGTAGAGCGGCTGCGGCGAATCAGCCCAGGGATCAACATAATAGCAATCAGCTCACGGGc                                                                    <  1:175037/80‑1 (MQ=255)     cGCATAAGCCCAGTGCAGTAGAGCGGCTGCGGCGAATCAGCCCAGGGATCAACATAATAGCAATCAGCTCACGGGCCAGAt                                                               <  2:175038/81‑1 (MQ=255)              ccAGTGCAGTAGAGCGGCTGCGGCGAATCAGCCCAGGGATCAACATAATAGCAATCAGCTCACGGGCCAGATCATTaaaaa                                                      <  1:175039/81‑1 (MQ=255)               cAGTGCAGTAGAGCGGCTGCGGCGAATCAGCCCAGGGATCAACATAATAGCAATCAGCTCACGGGCCAGATCATTaaaaaa                                                     <  2:175040/81‑1 (MQ=255)                  tGCAGTAGAGCGGCTGCGGCGAATCAGCCCAGGGATCAACATAATAGCAATCAGCTCACGGGCCAGATCATTAAAAAAcgc                                                  <  1:175041/81‑1 (MQ=255)                               cTGCGGCGAATCAGCCCAGGGATCAACATAATAGCAATCAGCTCACGGGCCAGATCATTAAAAAACGCCGCGCTCCCGAt                                      >  2:175069/1‑80 (MQ=255)                                   ggcgAATCAGCCCAGGGATCAACATAATAGCAATCAGCTCACGGGCCAGATCATTAAAAAACGCCGCGCTCCCGATTACCg                                 <  2:175042/81‑1 (MQ=255)                                        aTCAGCCCAGGGATCAACATAATAGCAATCAGCTCACGGGCCAGATCATTAAAAAACGCCGCGCTCCCGATTACCGGAcc                             >  1:175063/1‑80 (MQ=255)                                         tCAGCCCAGGGATCAACATAATAGCAATCAGCTCACGGGCCAGATCATTAAAAAACGCCGCGCTCCCGATTACCGGACCaa                           <  1:175043/81‑1 (MQ=255)                                              ccAGGGATCAACATAATAGCAATCAGCTCACGGGCCAGATCATTAAAAAACGCCGCGCTCCCGATTACCGGACCAAAAGAt                      >  2:175065/1‑81 (MQ=255)                                               cAGCGATCAACATAATAGCAATCAGCTCACGAGCCAGATCATTAAAAAACGCCGCGCTCCCGATTACCGGACCAAAAGAtt                     <  2:175044/81‑1 (MQ=255)                                                 gggATCAACATAATAGCAATCAGCTCACGGGCCAGATCATTAAAAAACGCCGCGCTCCCGATTACCGGACCAAAAGATTCg                   <  1:175045/81‑1 (MQ=255)                                                    aTCAACATAATAGCAATCAGCTCACGGGCCAGATCATTAAAAAACGCCGCGCTCCCGATTACCGGACCAAAAGATTCGGTc                <  1:175046/81‑1 (MQ=255)                                                       aaCATAATAGCAATCAGCTCACGGGCCAGATCATTAAAAAACGCCGCGCTCCCGATTACCGGACCAAAAGATTCGGTCaa              <  2:175047/80‑1 (MQ=255)                                                        aCATAATAGCAATCAGCTCACGGGCCAGATCATTAAAAAACGCCGCGCTCCCGATTACCGGACCAAAAGATTCGGTCAATa            <  2:175048/81‑1 (MQ=255)                                                          ataataGCAATCAGCTCACGGGCCAGATCATTAAAAAACGCCGCGCTCCCGATTACCGGACCAAAAGATTCGGTCAATaa           >  2:175062/1‑80 (MQ=255)                                                             ataGCAATCAGCTCACGGGCCAGATCATTAAAAAACGCCGCGCTCCCGATTACCGGACCAAAAGATTCGGTCAATAAAATa       >  2:175067/1‑81 (MQ=255)                                                                     cAGCTCACGGGCCAGATCATTAAAAAACGCCGCGCTCCCGATTACCGGACCAAAAGATTCGGTCAATAAAATACCGGa  >  1:175078/1‑78 (MQ=255)                                                                        |                                                                           GCACCGCATAAGCCCAGTGCAGTAGAGCGGCTGCGGCGAATCAGCCCAGGGATCAACATAATAGCAATCAGTTCACGGGCCAGATCATTAAAAAACGCCGCGCTCCCGATTACCGGACCAAAAGATTCGGTCAATAAAATACCGGA  >  NC_000913/914088‑914233

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑