Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	922,079	T→G	L578R (CTG→CGG)	macB →	ABC‑type tripartite efflux pump ATP binding/membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	922,079	0	T	G	100.0%	84.6 / NA	24	L578R (CTG→CGG)	macB	ABC‑type tripartite efflux pump ATP binding/membrane subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (9/15);  total (9/15)

CGGGAAATTGGCATTCGCATGGCTGTAGGTGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACTGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAACACTGTCACTGTTAATTGCTTTCACCTTGCAGCTTTTCTTACCC  >  NC_000913/922003‑922155                                                                             |                                                                             cgGGAAATTGGCATTCGCATGGCTGTAGGTGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACGGGtt                                                                          <  1:176318/81‑1 (MQ=255)      aaTTGGCATTCGCATGGCTGTAGGTGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACGGGTTTGCCt                                                                     <  1:176319/81‑1 (MQ=255)      aaTTGGCATTCGCATGGCTGTAGGTGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACGGGTTTGCCt                                                                     <  1:176320/81‑1 (MQ=255)           gCATTCGCATGGCTGTAGGTGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACGGGTTTGCCTGGTCg                                                                >  2:176358/1‑81 (MQ=255)             aTTCGCATGGCTGTAGGTGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACGGGTTTGCCTGGTCGGt                                                              <  2:176321/81‑1 (MQ=255)                  cATGGCTGTAGGTGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACGGGTTTGCCTGGTCGGTGgcgc                                                         <  2:176322/81‑1 (MQ=255)                      gCTGTAGGTGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACGGGTTTGCCTGGTCGGTGGCGCGtt                                                      <  1:176323/80‑1 (MQ=255)                          tAGGTGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACGGGTTTGCCTGGTCGGTGGCGCGTTGGGaa                                                 <  1:176326/81‑1 (MQ=255)                          tAGGTGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACGGGTTTGCCTGGTCGGTGGCGCGTTGGGaa                                                 <  2:176324/81‑1 (MQ=255)                          tAGGTGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACGGGTTTGCCTGGTCGGTGGCGCGTTGGGaa                                                 <  1:176325/81‑1 (MQ=255)                           aGGTGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACGGGTTTGCCTGGTCGGTGGCGCGTTGGGaa                                                 >  2:176354/1‑80 (MQ=255)                           aGGTGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACGGGTTTGCCTGGTCGGTGGCGCGTTGGGaa                                                 >  2:176355/1‑80 (MQ=255)                            ggTGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACGGGTTTGCCTGGTCGGTGGCGCGTTGGGAAt                                                <  1:176327/80‑1 (MQ=255)                                cgcgAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACGGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAacac                                           >  1:176359/1‑81 (MQ=255)                                   gAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACGGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAAcactgt                                        <  1:176328/81‑1 (MQ=255)                                               ttttGCAACAGTTCCTGATCGAAGCCGTACGGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAACACTGTCACTGTTAATTg                            >  1:176352/1‑81 (MQ=255)                                                tttGCAACAGTTCCTGATCGAAGCCGTACGGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAACACTGTCACTGTTAATTGc                           >  1:176361/1‑81 (MQ=255)                                                            ccTGATCGAAGCCGTACGGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAACACTGTCACTGTTAATTGCTTTCACCTTGc                <  2:176329/80‑1 (MQ=255)                                                               gATCGAAGCCGTACGGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAACACTGTCACTGTTAATTGCTTTCACCTTGCAGCt            >  1:176357/1‑81 (MQ=255)                                                                   gAAGCCGTACGGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAACACTGTCACTGTTAATTGCTTTCACCTTGCAGCtttt         <  2:176330/80‑1 (MQ=255)                                                                   gAAGCCGTACGGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAACACTGTCACTGTTAATTGCTTTCACCTTGCAGCtttt         <  2:176331/80‑1 (MQ=255)                                                                   gAAGCCGTACGGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAACACTGTCACTGTTAATTGCTTTCACCTTGCAGCTTTTc        >  1:176364/1‑81 (MQ=255)                                                                     aGCCGTACGGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAACACTGTCACTGTTAATTGCTTTCACCTTGCAGCTTTTCtt      <  1:176332/81‑1 (MQ=255)                                                                         gTACGGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAACACTGTCACTGTTAATTGCTTTCACCTTGCAGCTTTTCTTAccc  >  1:176362/1‑81 (MQ=255)                                                                             |                                                                             CGGGAAATTGGCATTCGCATGGCTGTAGGTGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACTGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAACACTGTCACTGTTAATTGCTTTCACCTTGCAGCTTTTCTTACCC  >  NC_000913/922003‑922155

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑