Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	996,703	C→G	G81A (GGC→GCC)	ssuA ←	aliphatic sulfonate ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	996,703	0	C	G	100.0%	71.0 / NA	21	G81A (GGC→GCC)	ssuA	aliphatic sulfonate ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (7/14);  total (7/14)

CGGTGGCTCGACGCCCACGTACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCCAGGAGATTT  >  NC_000913/996627‑996775                                                                             |                                                                         cGGTGGCTCGACGCCCACGTACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGGCga                                                                       <  1:188735/80‑1 (MQ=255) cGGTGGCTCGACGCCCACGTACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGGCga                                                                       <  1:188734/80‑1 (MQ=255)   gTGGCTCGACGCCCACGTACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGGCGAGAt                                                                    <  2:188736/81‑1 (MQ=255)    tGGCTCGACGCCCACGTACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATTTCCCCGGTACTGGCGAGATc                                                                   <  2:188737/81‑1 (MQ=255)      gCTCGACGCCCACGTACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGGCGAGATCa                                                                  <  2:188738/80‑1 (MQ=255)                   gTACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGGCGAGATCAATACTGCCAACGtt                                                    <  2:188739/81‑1 (MQ=255)                      cacCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGGCGAGATCAATACTGCCAACGTTTa                                                  <  2:188740/80‑1 (MQ=255)                               aGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGGCGAGATCAATACTGCCAACGTTTAACGCTTCCa                                         <  1:188741/80‑1 (MQ=255)                                     ggCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGGCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTg                                  <  1:188742/81‑1 (MQ=255)                                      gCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGGCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGc                                 >  1:188770/1‑81 (MQ=255)                                       cAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGGCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCg                                >  1:188766/1‑81 (MQ=255)                                       cAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGGCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCg                                <  2:188743/81‑1 (MQ=255)                                        aGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGGCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCgg                               <  2:188744/81‑1 (MQ=255)                                              ggCAAAGATTGGCGGAATATCCCCGGTACTGGCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCcgc                         >  1:188769/1‑81 (MQ=255)                                                cAAAGATTGGCGGAATATCCCCGGTACTGGCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCgg                       >  2:188771/1‑81 (MQ=255)                                                     aTTGGCGGAATATCCCCGGTACTGGCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAAc                  <  1:188745/81‑1 (MQ=255)                                                         gCGGAATATCCCCGGTACTGGCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTcc               <  2:188746/80‑1 (MQ=255)                                                                 tCCCCGGTACTGGCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCCAGgag      <  1:188747/81‑1 (MQ=255)                                                                    ccGGTACTGGCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCCAGGAGAtt   >  2:188775/1‑81 (MQ=255)                                                                     cGGTACTGGCGAGATCAATACTGCCCACGTTTAACTCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCCAGGAGAttt  >  1:188774/1‑81 (MQ=255)                                                                     cGGTACTGGCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCCAGGAGAttt  >  2:188767/1‑81 (MQ=255)                                                                             |                                                                         CGGTGGCTCGACGCCCACGTACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCCAGGAGATTT  >  NC_000913/996627‑996775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑