| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,007,421 | T→C | S60S (TCA→TCG) | ycbX ← | 6‑N‑hydroxylaminopurine resistance protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,007,421 | 0 | T | C | 80.0% | 85.0 / 17.7 | 40 | S60S (TCA→TCG) | ycbX | 6‑N‑hydroxylaminopurine resistance protein |
| Reads supporting (aligned to +/- strand): ref base T (1/7); new base C (18/14); total (19/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.61e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTGTGGCGAAATCAGCAAAACGAACATATGCGCTACTGCCATCTGGTGCGGTGAGATGCAAGCCATCATGCACAGGTGAAGGAGTAAACCGTACCATCTGGGGAAACTGGCGAGCGGTAATAAACGTACCGTCAGGTTCCGTGATCATAAAGAT > NC_000913/1007345‑1007498 | gtgtgGCGAAATCAGCAAAACGAACATATGCGCTACTGCCATCTGGTGCGGTGAGATGCAAGCCATCATGCACAGGCGAAg > 1:190221/1‑81 (MQ=255) tgtgGCGAAATCAGCAAAACGAACATATGCGCTACTGCCATCTGGTGCGGTGAGATGCAAGCCATCATGCACAGGTGAAg < 1:190189/80‑1 (MQ=255) tgtgGCGAAATCAGCAAAACGAACATATGCGCTACTGCCATCTGGTGCGGTGAGATGCAAGCCATCATGCACAGGCGAAgg < 2:190190/81‑1 (MQ=255) ggCGAAATCAGCAAAACGAACATATGCGCTACTGCCATCTGGTGCGGTGAGATGCAAGCCATCATGCACAGGCGAAGGAg > 1:190225/1‑80 (MQ=255) gCGAAATCAGCAAAACGAACATATGCGCTACTGCCATCTGGTGCGGTGAGATGCAAGCCATCATGCACAGGTGAAGGAGTa < 2:190191/81‑1 (MQ=255) gCGAAATCAGCAAAACGAACATATGCGCTACTGCCATCTGGTGCGGTGAGATGCAAGCCATCATGCACAGGCGAAGGAGt < 1:190192/80‑1 (MQ=255) gAAATCAGCAAAACGAACATATGCGCTACTGCCATCTGGTGCGGTGAGATGCAAGCCATCATGCACAGGCGAAGGAGTaaa > 2:190231/1‑81 (MQ=255) gAAATCAGCAAAACGAACATATGCGCTACTGCCATCTGGTGCGGTGAGATGCAAGCCATCATGCACAGGCGAAGGAGTaaa > 1:190226/1‑81 (MQ=255) aaTCAGCAAAACGAACATATGCGCTACTGCCATCTGGTGCGGTGAGATGCAAGCCATCATGCACAGGCGAAGGAGTAAAcc < 2:190193/81‑1 (MQ=255) aTCAGCAAAACGAACATATGCGCTACTGCCATCTGGTGCGGTGAGATGCAAGCCATCATGCACAGGCGAAGGAGTAAACCg > 1:190236/1‑81 (MQ=255) tCAGCAAAACGAACATATGCGCTACTGCCATCTGGTGCGGTGAGATGCAAGCCATCATGCACAGGCGAAGGAGTAAACCGt < 2:190194/81‑1 (MQ=255) aaaaCGAACATATGCGCTACTGCCATCTGGTGCGGTGAGATGCAAGCCATCATGCTCAGGCGAAGGAGTAAACCGTACCAt > 1:190250/1‑81 (MQ=255) aaaaCGAACATATGCGCTACTGCCATCTGGTGCGGTGAGATGCAAGCCATCATGCACAGGCGAAGGAGTAAACCGTACCAt < 2:190195/81‑1 (MQ=255) aaCATATGCGCTACTGCCATCTGGTGCGGTGAGATGCAAGCCATCATGCACAGGCGAAGGAGTAAACCGTACCATCTggg > 1:190232/1‑80 (MQ=255) aCATATGCGCTACTGCCATCTGGTGCGGTGAGATGCAAGCCATCATGCACAGGCGAAGGAGTAAACCGTACCATCTgggg > 1:190243/1‑80 (MQ=255) aCATATGCGCTACTGCCATCTGGTGCGGTGAGATGCAAGCCATCATGCACAGGCGAAGGAGTAAACCGTACCATCTgggg > 1:190240/1‑80 (MQ=255) atatGCGCTACTGCCATCTGGTGCGGTGAGATGCAAGCCATCATGCACAGGCGAAGGAGTAAACCGTACCATCTGGGGaaa > 2:190247/1‑81 (MQ=255) tatGCGCTACTGCCATCTGGTGCGGTGAGATGCAAGCCATCATGCACAGGCGAAGGAGTAAACCGTACCATCTGGGGAAAc < 1:190196/81‑1 (MQ=255) atGCGCTACTGCCATCTGGTGCGGTGAGATGCAAGCCATCATGCACAGGCGAAGGAGTAAACCGTACCATCTGGGGAAACt > 1:190235/1‑81 (MQ=255) tGCGCTACTGCCATCTGGTGCGGTGAGATGCAAGCCATCATGCACAGGCGAAGGAGTAAACCGTACCATCTGGGGAAACt < 2:190197/80‑1 (MQ=255) tGCGCTACTGCCATCTGGTGCGGTGAGATGCAAGCCATCATGCACAGGCGAAGGAGTAAACCGTACCATCTGGGGAAACt < 1:190198/80‑1 (MQ=255) gcgcTACTGCCATCTGGTGCGGTGAGATGCAAGCCATCATGCACAGGCGAAGGAGTAAACCGTACCATCTGGGGAAACTgg > 2:190237/1‑81 (MQ=255) gcTACTGCCATCTGGTGCGGTGAGATGCAAGCCATCATGCACAGGTGAAGGAGTAAACCGTACCATCTGGGGAAACTGGCg < 1:190199/81‑1 (MQ=255) cTGCCATCTGGTGCGGTGAGATGCAAGCCATCATGCACAGGTGAAGGAGTAAACCGTACCATCTGGGGAAACTGGCGAGc < 1:190201/80‑1 (MQ=255) cTGCCATCTGGTGCGGTGAGATGCAAGCCATCATGCACAGGTGAAGGAGTAAACCGTACCATCTGGGGAAACTGGCGAGCg < 1:190200/81‑1 (MQ=255) cTGGTGCGGTGAGATGCAAGCCATCATGCACAGGTGAAGGAGTAAACCGCACCATCTGGGGAAACTGGCGAGCGGtaata > 2:190272/1‑80 (MQ=255) cTGGTGCGGTGAGATGCAAGCCATCATGCACAGGCGAAGGAGTAAACCGTACCATCTGGGGAAACTGGCGAGCGGtaata > 1:190239/1‑80 (MQ=255) cTGGTGCGGTGAGATGCAAGCCATCATGCACAGGCGAAGGAGTAAACCGTACCATCTGGGGAAACTGGCGAGCGGtaata > 2:190241/1‑80 (MQ=255) gTGCGGTGAGATGCAAGCCATCATGCACAGGCGAAGGAGTAAACCGTACCATCTGGGGAAACTGGCGAGCGGTAATAAAc < 1:190202/80‑1 (MQ=255) gTGCGGTGAGATGCAAGCCATCATGCACAGGCGAAGGAGTAAACCGTACCATCTGGGGAAACTGGCGAGCGGTAATAAACg > 1:190242/1‑81 (MQ=255) cGGTGAGATGCAAGCCATCATGCACAGGCGAAGGAGTAAACCGTACCATCTGGGGAAACTGGCGAGCGGTAATAAACGTAc < 2:190203/81‑1 (MQ=255) ggTGAGATGCAAGCCATCATGCACAGGCGAAGGAGTAAACCGTACCATCTGGGGAAACTGGCGAGCGGTAATAAACGTAcc < 2:190204/81‑1 (MQ=255) gaTGCAAGCCATCATGCACAGGCGAAGGAGTAAACCGTACCATCTGGGGAAACTGGCGAGCGGTAATAAACGTACCGTCAg < 2:190205/81‑1 (MQ=255) aTGCAAGCCATCATGCACAGGCGAAGGAGTAAACCGTACCATCTGGGGAAACTGGCGAGCGGTAATAAACGTACCGTCAgg < 2:190206/81‑1 (MQ=255) aaGCCATCATGCACAGGCGAAGGAGTAAACCGTACCATCTGGGGAAACTGGCGAGCGGTAATAAACGTACCGTCAGGTTcc < 1:190207/81‑1 (MQ=255) aGCCATCATGCACAGGCGAAGGAGTAAACCGTACCATCTGGGGAAACTGGCGAGCGGTAATAAACGTACCGTCAGGTTCCg > 1:190248/1‑81 (MQ=255) atcatGCACAGGTGAAGGAGTAAACCGTACCATCTGGGGAAACTGGCGAGCGGTAATAAACGTACCGTCAGGTTCCGTGAt < 1:190208/81‑1 (MQ=255) atcatGCACAGGCGAAGGAGTAAACCGTACCATCTGGGGAAACTGGCGAGCGGTAATAAACGTACCGTCAGGTTCCGTGAt > 1:190259/1‑81 (MQ=255) acaGGTGAAGGAGTAAACCGTACCATCTGGGGAAACTGGCGAGCGGTAATAAACGTACCGTCAGGTTCCGTGATCATAAAg < 2:190209/81‑1 (MQ=255) aGGCGAAGGAGTAAACCGTACCATCTGGGGAAACTGGCGAGCGGTAATAAACGTACCGTCAGGTTCCGTGATCATAAAGAt > 2:190245/1‑81 (MQ=255) | GTGTGGCGAAATCAGCAAAACGAACATATGCGCTACTGCCATCTGGTGCGGTGAGATGCAAGCCATCATGCACAGGTGAAGGAGTAAACCGTACCATCTGGGGAAACTGGCGAGCGGTAATAAACGTACCGTCAGGTTCCGTGATCATAAAGAT > NC_000913/1007345‑1007498 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |