Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,026,163	A→C	I565L (ATC→CTC)	helD →	DNA helicase IV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,026,163	0	A	C	100.0%	114.0 / NA	32	I565L (ATC→CTC)	helD	DNA helicase IV
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (22/10);  total (22/10)

GTTATTGGATGAGAGTCAACTTGACGCTTTGCTGGATAAGCTCTCTGGTTATGCCAAACCGGAAGAGCGCATTCTGATCCTGGCGCGTTACCATCACATGAGGCCTGCCAGCCTGGAAAAAGCGGCAACACGCTGGCCGAAGTTGCAAATCGA  >  NC_000913/1026087‑1026239                                                                             |                                                                             gTTATTGGATGAGAGTCAACTTGACGCTTTGCTGGATAAGCTCTCTGGTTATGCCAAACCGGAAGAGCGCATTCTGCTCCt                                                                          <  1:193260/81‑1 (MQ=255)  ttATTGGATGAGAGTCAACTTGACGCTTTGCTGGATAAGCTCTCTGGTTATGCCAAACCGGAAGAGCGCATTCTGCTCCTg                                                                         <  2:193261/81‑1 (MQ=255)    aTTGGATGAGAGTCAACTTGACGCTTTGCTGGATAAGCTCTCTGGTTATGCCAAACCGGAAGAGCGCATTCTGCTCCTGgc                                                                       >  1:193286/1‑81 (MQ=255)         aTGAGAGTCAACTTGACGCTTTGCTGGATAAGCTCTCTGGTTATGCCAAACCGGAAGAGCGCATTCTGCTCCTGGCGCGt                                                                   >  2:193287/1‑80 (MQ=255)             gagTCAACTTGACGCTTTGCTGGATAAGCTCTCTGGTTATGCCAAACCGGAAGAGCGCATTCTGCTCCTGGCGCGTTACCa                                                              >  2:193285/1‑81 (MQ=255)                 cAACTTGACGCTTTGCTGGATAAGCTCTCTGGTTATGCCAAACCGGAAGAGCGCATTCTGCTCCTGGCGCGTTACCATca                                                           >  1:193284/1‑80 (MQ=255)                 cAACTTGACGCTTTGCTGGATAAGCTCTCTGGTTATGCCAAACCGGAAGAGCGCATTCTGCTCCTGGCGCGTTACCATca                                                           >  1:193300/1‑80 (MQ=255)                   aCTTGACGCTTTGCTGGATAAGCTCTCTGGTTATGCCAAACCGGAAGAGCGCATTCTGCTCCTGGCGCGTTACCATCACAt                                                        >  1:193289/1‑81 (MQ=255)                   aCTTGACGCTTTGCTGGATAAGCTCTCTGGTTATGCCAAACCGGAAGAGCGCATTCTGCTCCTGGCGCGTTACCATCACAt                                                        >  2:193295/1‑81 (MQ=255)                    cTTGACGCTTTGCTGGATAAGCTCTCTGGTTATGCCAAACCGGAAGAGCGCATTCTGCTCCTGGCGCGTTACCATCACAt                                                        >  1:193283/1‑80 (MQ=255)                        aCGCTTTGCTGGATAAGCTCTCTGGTTATGCCAAACCGGAAGAGCGCATTCTGCTCCTGGCGCGTTACCATCACATGAGGc                                                   <  2:193263/81‑1 (MQ=255)                        aCGCTTTGCTGGATAAGCTCTCTGGTTATGCCAAACCGGAAGAGCGCATTCTGCTCCTGGCGCGTTACCATCACATGAGGc                                                   <  1:193262/81‑1 (MQ=255)                          gCTTTGCTGGATAAGCTCTCTGGTTATGCCAAACCGGAAGAGCGCATTCTGCTCCTGGCGCGTTACCATCACATGAGGCCt                                                 <  1:193264/81‑1 (MQ=255)                          gCTTTGCTGGATAAGCTCTCTGGTTATGCCAAACCGGAAGAGCGCATTCTGCTCCTGGCGCGTTACCATCACATGAGGCCt                                                 >  1:193303/1‑81 (MQ=255)                             ttGCTGGATAAGCTCTCTGGTTATGCCAAACCGGAAGAGCGCATTCTGCTCCTGGCGCGTTACCATCACATGAGGCCTGc                                               >  2:193301/1‑80 (MQ=255)                              tGCTGGATAAGCTCTCTGGTTATGCCAAACCGGAAGAGCGCATTCTGCTCCTGGCGCGTTACCATCACATGAGGCCTGcc                                              <  1:193265/80‑1 (MQ=255)                                       aGCTCTCTGGTTATGCCAAACCGGAAGAGCGCATTCTGCTCCTGGCGCGTTACCATCACATGAGGCCTGCCAGCCTGGaa                                     >  1:193296/1‑80 (MQ=255)                                          tctctGGTTATGCCAAACCGGAAGAGCGCATTCTGCTCCTGGCGCGTTACCATCACATGAGGCCTGCCAGCCTGGAAAAAg                                 >  2:193302/1‑81 (MQ=255)                                           ctctGGTTATGCCAAACCGGAAGAGCGCATTCTGCTCCTGGCGCGTTACCATCACATGAGGCCTGCCAGCCTGGAAAAAGc                                >  2:193292/1‑81 (MQ=255)                                                gTTATGCCAAACCGGAAGAGCGCATTCTGCTCCTGGCGCGTTACCATCACATGAGGCCTGCCAGCCTGGAAAAAGCGGc                             <  1:193267/79‑1 (MQ=255)                                                gTTATGCCAAACCGGAAGAGCGCATTCTGCTCCTGGCGCGTTACCATCACATGAGGCCTGCCAGCCTGGAAAAAGCGGc                             <  1:193266/79‑1 (MQ=255)                                                 ttATGCCAAACCGGAAGAGCGCATTCTGCTCCTGGCGCGTTACCATCACATGAGGCCTGCCAGCCTGGAAAAAGCGGCaa                           >  2:193294/1‑80 (MQ=255)                                                   aTGCCAAACCGGAAGAGCGCATTCTGCTCCTGGCGCGTTACCATCACATGAGGCCTGCCAGCCTGGAAAAAGCGGCAacac                        >  2:193298/1‑81 (MQ=255)                                                   aTGCCAAACCGGAAGAGCGCATTCTGCTCCTGGCGCGTTACCATCACATGAGGCCTGCCAGCCTGGAAAAAGCGGCAacac                        >  2:193304/1‑81 (MQ=255)                                                     gCCAAACCGGAAGAGCGCATTCTGCTCCTGGCGCGTTACCATCACATGAGGCCTGCCAGCCTGGAAAAAGCGGCAACACGc                      >  2:193299/1‑81 (MQ=255)                                                      ccAAACCGGAAGAGCGCATTCTGCTCCTGGCGCGTTACCATCACATGAGGCCTGCCAGCCTGGAAAAAGCGGCAACACGCt                     >  1:193293/1‑81 (MQ=255)                                                              gAAGAGCGCATTCTGCTCCTGGCGCGTTACCATCACATGAGGCCTGCCAGCCTGGAAAAAGCGGCAACACGCTGGCCGAAg             >  1:193314/1‑81 (MQ=255)                                                              gAAGAGCGCATTCTGCTCCTGGCGCGTTACCATCACATGAGGCCTGCCAGCCTGGAAAAAGCGGCAACACGCTGGCCGAAg             <  1:193268/81‑1 (MQ=255)                                                                   gcgcATTCTGCTCCTGGCGCGTTACCATCACATGAGGCCTGCCAGCCTGGAAAAAGCGGCAACACGCTGGCCGAAGTTGc         >  2:193291/1‑80 (MQ=255)                                                                     gcATTCTGCTCCTGGCGCGTTACCATCACATGAGGCCTGCCAGCCTGGAAAAAGCGGCAACACGCTGGCCGAAGTTGCaaa      <  2:193269/81‑1 (MQ=255)                                                                      cATTCTGCTCCTGGCGCGTTACCATCACATGAGGCCTGCCAGCCTGGAAAAAGCGGCAACACGCTGGCCGAAGTTGCAAAt     >  2:193290/1‑81 (MQ=255)                                                                         tCTGCTCCTGGCGCGTTACCATCACATGAGGCCTGCCAGCCTGGAAAAAGCGGCAACACGCTGGCCGAAGTTGCAAATCGa  >  1:193316/1‑81 (MQ=255)                                                                             |                                                                             GTTATTGGATGAGAGTCAACTTGACGCTTTGCTGGATAAGCTCTCTGGTTATGCCAAACCGGAAGAGCGCATTCTGATCCTGGCGCGTTACCATCACATGAGGCCTGCCAGCCTGGAAAAAGCGGCAACACGCTGGCCGAAGTTGCAAATCGA  >  NC_000913/1026087‑1026239

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑