Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,042,554	C→G	A553P (GCA→CCA)	etk ←	protein‑tyrosine kinase Etk

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,042,554	0	C	G	100.0%	84.0 / NA	24	A553P (GCA→CCA)	etk	protein‑tyrosine kinase Etk
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (11/13);  total (11/13)

GAATAACCACGGCGTAAGTCGGCATCAATAAATAACACTTTTTGATCGGACTGGGCGATCACCGCTGCCAGAGTTGAACTGACAAACGTTTTACCACTGTCTGGCGTCGCACCGGTGATCATTAGAATGTTATTCTCCGTCTCCA  >  NC_000913/1042487‑1042631                                                                    |                                                                              gAATAACCACGGCGTAAGTCGGCATCAATAAATAACACTTTTTGATCGGACTGGGCGATCACCGCTGGCAGAGTTGAACt                                                                   >  1:196082/1‑80 (MQ=255)    tAACCACGGCGTAAGTCGGCATCAATAAATAACACTTTTTGATCGGACTGGGCGATCACCGCTGGCAGAGTTGAACTGAc                                                                >  1:196087/1‑80 (MQ=255)    tAACCACGGCGTAAGTCGGCATCAATAAATAACACTTTTTGATCGGACTGGGCGATCACCGCTGGCAGAGTTGAACTGACa                                                               <  2:196052/81‑1 (MQ=255)     aaCCACGGCGTAAGTCGGCATCAATAAATAACACTTTTTGATCGGACTGGGCGATCACCGCTGGCAGAGTTGAACTGACa                                                               >  1:196079/1‑80 (MQ=255)          cGGCGTAAGTCGGCATCAATAAATAACACTTTTTGATCGGACTGGGCGATCACCGCTGGCAGAGTTGAACTGACAAACGtt                                                         >  1:196076/1‑81 (MQ=255)          cGGCGTAAGTCGGCATCAATAAATAACACTTTTTGATCGGACTGGGCGATCACCGCTGGCAGAGTTGAACTGACAAACGtt                                                         <  1:196053/81‑1 (MQ=255)            gCGTAAGTCGGCATCAATAAATAACACTTTTTGATCGGACTGGGCGATCACCGCTGGCAGAGTTGAACTGACAAACGtttt                                                       <  2:196054/81‑1 (MQ=255)                aaGTCGGCATCAATAAATAACACTTTTTGATCGGACTGGGCGATCACCGCTGGCAGAGTTGAACTGACAAACGTTTTACCa                                                   >  2:196080/1‑81 (MQ=255)                 aGTCGGCATCAATAAATAACACTTTTTGATCGGACTGGGCGATCACCGCTGGCAGAGTTGAACTGACAAACGTTTTACCAc                                                  <  1:196055/81‑1 (MQ=255)                      gCATCAATAAATAACACTTTTTGATCGGACTGGGCGATCACCGCTGGCAGAGTTGAACTGACAAACGTTTTACCACTGTCt                                             <  2:196056/81‑1 (MQ=255)                           aataaataACACTTTTTGATCGGACTGGGCGATCACCGCTGGCAGAGTTGAACTGACAAACGTTTTACCACTGTCTGGCGt                                        >  1:196084/1‑81 (MQ=255)                            ataaataaCACTTTTTGATCGGACTGGGCGATCACCGCTGGCAGAGTTGAACTGACAAACGTTTTACCACTGTCTGGCGTc                                       >  1:196057/1‑81 (MQ=255)                             taaataaCACTTTTTGATCGGACTGGGCGATCACCGCTGGCAGAGTTGAACTGACAAACGTTTTACCACTGTCTGGCGTCg                                      >  1:196086/1‑81 (MQ=255)                                         tttGATCGGACTGGGCGATCACCGCTGGCAGAGTTGAACTGACAAACGTTTTACCACTGTCTGGCGTCGCACCGGTGATc                           <  1:196059/80‑1 (MQ=255)                                         tttGATCGGACTGGGCGATCACCGCTGGCAGAGTTGAACTGACAAACGTTTTACCACTGTCTGGCGTCGCACCGGTGATc                           <  1:196058/80‑1 (MQ=255)                                                ggACTGGGCGATCACCGCTGGCAGAGTTGAACTGACAAACGTTTTACCACTGTCTGGCGTCGCACCGGTGATCATTAGAAt                   >  2:196091/1‑81 (MQ=255)                                                  aCTGGGCGATCACCGCTGGCAGAGTTGAACTGACAAACGTTTTACCACTGTCTGGCGTCGCACCGGTGATCATTAGAATGt                 <  2:196060/81‑1 (MQ=255)                                                  aCTGGGCGATCACCGCTGGCAGAGTTGAACTGACAAACGTTTTACCACTGTCTGGCGTCGCACCGGTGATCATTAGAATGt                 >  2:196089/1‑81 (MQ=255)                                                   cTGGGCGATCACCGCTGGCAGAGTTGAACTGACAAACGTTTTACCACTGTCTGGCGTCGCACCGGTGATCATTAGAATGtt                <  2:196061/81‑1 (MQ=255)                                                         gATCACCGCTGGCAGAGTTGAACTGACAAACGTTTTACCACTGTCTGGCGTCGCACCGGTGATCATTAGAATGTTATtctc          >  1:196085/1‑81 (MQ=255)                                                         gATCACCGCTGGCAGAGTTGAACTGACAAACGTTTTACCACTGTCTGGCGTCGCACCGGTGATCATTAGAATGTTATtctc          <  1:196062/81‑1 (MQ=255)                                                           tCACCGCTGGCAGAGTTGAACTGACAAACGTTTTACCACTGTCTGGCGTCGCACCGGTGATCATTAGAATGTTATTCTCCg        <  2:196063/81‑1 (MQ=255)                                                                gCTGGCAGAGTTGAACTGACAAACGTTTTACCACTGTCTGGCGTCGCACCGGTGATCATTAGAATGTTATTCTCCGTCTcc   <  1:196064/81‑1 (MQ=255)                                                                 cTGGCAGAGTTGAACTGACAAACGTTTTACCACTGTCTGGCGTCGCACCGGTGATCATTAGAATGTTATTCTCCGTCTCca  <  2:196065/81‑1 (MQ=255)                                                                    |                                                                              GAATAACCACGGCGTAAGTCGGCATCAATAAATAACACTTTTTGATCGGACTGGGCGATCACCGCTGCCAGAGTTGAACTGACAAACGTTTTACCACTGTCTGGCGTCGCACCGGTGATCATTAGAATGTTATTCTCCGTCTCCA  >  NC_000913/1042487‑1042631

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑