Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,096,234	C→G	P131R (CCG→CGG)	ycdU →	uncharacterized protein YcdU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,096,234	0	C	G	100.0%	35.9 / NA	11	P131R (CCG→CGG)	ycdU	uncharacterized protein YcdU
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (5/6);  total (5/6)

GTGCAATGAGTTGGGGGCTATACCAGCTTCTACCCTCGTTATATGCGCTGTTCCTGATTTCGTTACTTGGGCCGTTGGTCATCTTTCTGCTGTTTCGGATGATCCGCACCAGTTATGTCAAGCAGGTCGCTATTTCAGTAGCGAAA  >  NC_000913/1096162‑1096307                                                                         |                                                                          gTGCAATGAGTTGGGGGCTATACCAGCTTCTACCCTCGTTATATGCGCTGTTCCTGATTTCGTTACTTGGGCGGTTGGTCa                                                                   <  1:204223/81‑1 (MQ=255) gTGCAATGAGTTGGGGGCTATACCAGCTTCTACCCTCGTTATATGCGCTGTTCCTGATTTCGTTACTTGGGCGGTTGGTCa                                                                   >  2:204240/1‑81 (MQ=255)     aaTGAGTTGGGGGCTATACCAGCTTCTACCCTCGTTATATGCGCTGTTCCTGATTTCGTTACTTGGGCGGTTGGTCATCtt                                                               >  1:204234/1‑81 (MQ=255)                                  ccTCGTTATATGCGCTGTTCCTGATTTCGTTACTTGGGCGGTTGGTCATCTTTCTGCTGTTTCGGATGATCCGCACCAGt                                   >  2:204235/1‑80 (MQ=255)                                         atatGCGCTGTTCCTGATTTCGTTACTTGGGCGGTTGGTCATCTTTCTGCTGTTTCGGATGATCCGCACCAGTTATGTCa                            <  1:204224/80‑1 (MQ=255)                                           atGCGCTGTTCCTGATTTCGTTACTTGGGCGGTTGGTCATCTTTCTGCTGTTTCGGATGATCCGCACCAGTTATGTCAAg                          >  1:204242/1‑80 (MQ=255)                                                   ttCCTGATTTCGTTACTTGGGCGGTTGGTCATCTTTCTGCTGTTTCGGATGATCCGCACCAGTTATGTCAAGCAGGTCGc                  >  1:204239/1‑80 (MQ=255)                                                          tttCGTTACTTGGGCGGTTGGTCATCTTTCTGCTGTTTCGGATGATCCGCACCAGTTATGTCAAGCAGGTCGCTATTTCAg          <  1:368840/81‑1 (MQ=255)                                                                 aCTTGGGCGGTTGGTCATCTTTCTGCTGTTTCGGATGATCCGCACCAGTTATGTCAAGCAGGTCGCTATTTCAGTAGCGa    <  1:204225/80‑1 (MQ=255)                                                                  cTTGGGCGGTTGGTCATCTTTCTGCTGTTTCGGATGATCCGCACCAGTTATGTCAAGCAGGTCGCTATTTCAGTAGCGaaa  <  1:204226/81‑1 (MQ=255)                                                                  cTTGGGCGGTTGGTCATCTTTCTGCTGTTTCGGATGATCCGCACCAGTTATGTCAAGCAGGTCGCTATTTCAGTAGCGaa   <  2:204227/80‑1 (MQ=255)                                                                         |                                                                          GTGCAATGAGTTGGGGGCTATACCAGCTTCTACCCTCGTTATATGCGCTGTTCCTGATTTCGTTACTTGGGCCGTTGGTCATCTTTCTGCTGTTTCGGATGATCCGCACCAGTTATGTCAAGCAGGTCGCTATTTCAGTAGCGAAA  >  NC_000913/1096162‑1096307

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑