Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,143,915	T→A	L151F (TTA→TTT)	rne ←	ribonuclease E

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,143,915	0	T	A	100.0%	98.5 / NA	27	L151F (TTA→TTT)	rne	ribonuclease E
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (10/17);  total (10/17)

AGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGA  >  NC_000913/1143840‑1143984                                                                            |                                                                      agATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTAAATTc                                                                  >  2:211488/1‑81 (MQ=255)     ttGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTAAATTCGGTa                                                              >  2:211485/1‑81 (MQ=255)       gCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTAAATTCGGTACg                                                            >  1:211495/1‑81 (MQ=255)        ccGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTAAATTCGGTACg                                                            <  1:211450/80‑1 (MQ=255)         cGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTAAATTCGGTACGgt                                                          <  1:211451/81‑1 (MQ=255)          gACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTAAATTCGGTACGgtc                                                         >  2:211493/1‑81 (MQ=255)          gACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTAAATTCGGTACGgtc                                                         <  2:211452/81‑1 (MQ=255)           aCGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTAAATTCGGTACGgtcg                                                        <  1:211453/81‑1 (MQ=255)            cGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTAAATTCGGTACGgtcgt                                                       <  2:211454/81‑1 (MQ=255)               cAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTAAATTCGGTACGGTCGTCGc                                                    <  2:211455/81‑1 (MQ=255)                        gcACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTAAATTCGGTACGGTCGTCGCCTTCGATAc                                           >  2:211491/1‑81 (MQ=255)                        gcACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTAAATTCGGTACGGTCGTCGCCTTCGATAc                                           <  2:211456/81‑1 (MQ=255)                           cGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTAAATTCGGTACGGTCGTCGCCTTCGATACGGc                                        >  2:211503/1‑81 (MQ=255)                            gATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTAAATTCGGTACGGTCGTCGCCTTCGATACGGc                                        >  1:211483/1‑80 (MQ=255)                             aTAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTAAATTCGGTACGGTCGTCGCCTTCGATACGGc                                        >  2:211497/1‑79 (MQ=255)                                aGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTAAATTCGGTACGGTCGTCGCCTTCGATACGGCgaga                                    <  2:211457/80‑1 (MQ=255)                                aGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTAAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGaa                                   <  1:211458/81‑1 (MQ=255)                                  ccccATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTAAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAAt                                 <  1:211459/81‑1 (MQ=255)                                            tCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTAAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCcgcg                       >  1:211492/1‑81 (MQ=255)                                             ccGGCAGTTCAAGGCTTGCCAGTGCTTCTTTAAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCcgcg                       <  1:211460/80‑1 (MQ=255)                                              cGGCAGTTCAAGGCTTGCCAGTGCTTCTTTAAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCcgcgc                      <  1:211461/80‑1 (MQ=255)                                               ggCAGTTCAAGGCTTGCCAGTGCTTCTTTAAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCcgcgcgc                    <  2:211462/81‑1 (MQ=255)                                                       aaGGCTTGCCAGTGCTTCTTTAAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGgttgtt            <  1:211463/81‑1 (MQ=255)                                                               ccAGTGCTTCTTTAAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATc     <  1:211464/80‑1 (MQ=255)                                                                cAGTGCTTCTTTAAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCAt      >  2:211494/1‑78 (MQ=255)                                                                cAGTGCTTCTTTAAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAg   <  1:211465/81‑1 (MQ=255)                                                                 aGTGCTTCTTTAAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGa  <  1:211466/81‑1 (MQ=255)                                                                            |                                                                      AGATTTGCCGACGCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGA  >  NC_000913/1143840‑1143984

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑