Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,182,052	C→G	A260P (GCG→CCG)	potD ←	spermidine preferential ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,182,052	0	C	G	100.0%	72.4 / NA	21	A260P (GCG→CCG)	potD	spermidine preferential ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (12/9);  total (12/9)

CACATCCGGGCGCAGCAGGAAGTTGATCAATTTCAGCGCGCCTTCTTTGTTTTTGGCATTTGCCGGGATCGCCAGGCTGTCCATCCAGAAAATGCCGCCTTCTTTCGGCCACACCACGTCAATTGGCGTACCCGCCTGGCGTGCAACG  >  NC_000913/1181981‑1182128                                                                        |                                                                             cacaTCCGGGCGCAGCAGGAAGTTGATCAATTTCAGCGCGCCTTCTTTGTTTTTGGCATTTGCCGGGATCGGCAGGCTGtc                                                                     >  1:217390/1‑81 (MQ=255)  acaTCCGGGCGCAGCAGGAAGTTGATCAATTTCAGCGCGCCTTCTTTGTTTTTGGCATTTGCCGGGATCGGCAGGCTGtcc                                                                    <  2:217355/81‑1 (MQ=255)    aTCCGGGCGCAGCAGGAAGTTGATCAATTTCAGCGCGCCTTCTTTGTTTTTGGCATTTGCCGGGATCGGCAGGCTGtccat                                                                  >  2:217396/1‑81 (MQ=255)             cagcagGAAGTTGATCAATTTCAGCGCGCCTTCTTTGTTTTTGGCATTTGCCGGGATCGGCAGGCTGTCCATCCAGAAAAt                                                         >  1:217391/1‑81 (MQ=255)              agcagGAAGTTGATCAATTTCAGCGCGCCTTCTTTGTTTTTGGCATTTGCCGGGATCGGCAGGCTGTCCATCCAGAAAATg                                                        <  1:217356/81‑1 (MQ=255)                             aaTTTCAGCGCGCCTTCTTTGTTTTTGGCATTTGCCGGGATCGGCAGGCTGTCCATCCAGAAAATGCCGCCTTCTTTCgg                                          <  2:217357/80‑1 (MQ=255)                             aaTTTCAGCGCGCCTTCTTTGTTTTTGGCATTTGCCGGGATCGGCAGGCTGTCCATCCAGAAAATGCCGCCTTCTTTCGGc                                         >  2:217389/1‑81 (MQ=255)                                 tCAGCGCGCCTTCTTTGTTTTTGGCATTTGCCGGGATCGGCAGGCTGTCCATCCAGAAAATGCCGCCTTCTTTCGGCcaca                                     >  1:217395/1‑81 (MQ=255)                                 tCAGCGCGCCTTCTTTGTTTTTAGCATTTGCCGGGATCGGCAGGCTGTCCATCCAGAAAATGCCGCCTTCTTTCGGCcac                                      <  2:217358/80‑1 (MQ=255)                                      gcgcCTTCTTTGTTTTTGGCATTTGCCGGGATCGGCAGGCTGTCCATCCAGAAAATGCCGCCTTCTTTCGGCCACACCACg                                <  2:217359/81‑1 (MQ=255)                                          cttcttTGTTTTTGGCATTTGCCGGGATCGGCAGGCTGTCCATCCAGAAAATGCCGCCTTCTTTCGGCCACACCACGTCaa                            >  1:217410/1‑81 (MQ=255)                                           ttcttTGTTTTTGGCATTTGCCGGGATCGGCAGGCTGTCCATCCAGAACATGCCGCCTTCTTTCGGCCACACCACGTCAAt                           >  2:217398/1‑81 (MQ=255)                                                  tttttGGCATTTGCCGGGATCGGCAGGCTGTCCATCCAGAAAATGCCGCCTTCTTTCGGCCACACCACGTCAATTGGCGt                     <  2:217360/80‑1 (MQ=255)                                                         cATTTGCCGGGATCGGCAGGCTGTCCATCCAGAAAATGCCGCCTTCTTTCGGCCACACCACGTCAATTGGCGTACCCGCCt             <  1:217361/81‑1 (MQ=255)                                                         cATTTGCCGGGATCGGCAGGCTGTCCATCCAGAAAATGCCGCCTTCTTTCGGCCACACCACGTCAATTGGCGTACCCGCCt             <  2:217362/81‑1 (MQ=255)                                                             tGCCGGGATCGGCAGGCTGTCCATCCAGAAAATGCCGCCTTCTTTCGGCCACACCACGTCAATTGGCGTACCCGCCTGGCg         >  1:217404/1‑81 (MQ=255)                                                             tGCCGGGATCGGCAGGCTGTCCATCCAGAAAATGCCGCCTTCTTTCGGCCACACCACGTCAATTGGCGTACCCGCCTGGCg         >  2:217399/1‑81 (MQ=255)                                                              gCCGGGATCGGCAGGCTGTCCATCCAGAAAATGCCGCCTTCTTTCGGCCACACCACGTCAATTGGCGTACCCGCCTGGCGt        >  1:217397/1‑81 (MQ=255)                                                                cGGGATCGGCAGGCTGTCCATCCAGAAAATGCCGCCTTCTTTCGGCCACACCACGTCAATTGGCGTACCCGCCTGGCGTg       >  2:217392/1‑80 (MQ=255)                                                                  ggATCGGCAGGCTGTCCATCCAGAAAATGCCGCCTTCTTTCGGCCACACCACGTCAATTGGCGTACCCGCCTGGCGTGCaa    <  1:217363/81‑1 (MQ=255)                                                                     tCGGCAGGCTGTCCATCCAGAAAATGCCGCCTTCTTTCGGCCACACCACGTCAATTGGCGTACCCGCCTGGCGTGCAACg  >  1:217411/1‑80 (MQ=255)                                                                        |                                                                             CACATCCGGGCGCAGCAGGAAGTTGATCAATTTCAGCGCGCCTTCTTTGTTTTTGGCATTTGCCGGGATCGCCAGGCTGTCCATCCAGAAAATGCCGCCTTCTTTCGGCCACACCACGTCAATTGGCGTACCCGCCTGGCGTGCAACG  >  NC_000913/1181981‑1182128

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑