Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,198,448	G→A	intergenic (‑211/‑247)	ymfE ← / → lit	e14 prophage; uncharacterized protein YmfE/e14 prophage; cell death peptidase Lit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,198,448	0	G	A	100.0%	37.7 / NA	11	intergenic (‑211/‑247)	ymfE/lit	e14 prophage; uncharacterized protein YmfE/e14 prophage; cell death peptidase Lit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/7);  total (4/7)

ATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTG  >  NC_000913/1198380‑1198519                                                                     |                                                                        aTCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAaatgaaataataaaataatgaaat                                                             >  2:219784/1‑81 (MQ=255)               gcgcTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATAAAATAatgaaatgatgaaatgatgaa                                               <  2:219765/81‑1 (MQ=255)                cgcTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATAAAATAatgaaatgatgaaatgatgaaa                                              <  2:219766/81‑1 (MQ=255)                           ttaCGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATAAAATAatgaaatgatgaaatgatgaaatgatgaaatg                                    <  1:219767/78‑1 (MQ=255)                           tGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATAAAATAatgaaatgatgaaatgatgaaatgatgaaatg                                    <  2:219768/80‑1 (MQ=255)                             aCGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATAAAATAatgaaatgatgaaatgatgaaatgatgaaatgat                                  <  1:219769/80‑1 (MQ=255)                              cGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATAAAATAatgaaatgatgaaatgatgaaatgatgaaatgatga                                <  1:219770/81‑1 (MQ=255)                                             tGAAATTGATAGAAATGAAATAATAAAATAATgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgat                  >  1:219787/1‑80 (MQ=255)                                               aaaTTGATAGAAATGAAATAATAAAATAATgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgat                  <  2:219771/78‑1 (MQ=255)                                               aaaTTGATAGAAATGAAATAATAAAATAATGAaatgatgaaatgatgaaatgatgaaatgatgaaatgatgaaatgatga                >  2:219786/1‑80 (MQ=255)                                                   tGATAGAAATGAAATAATAAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACagag           >  1:219788/1‑81 (MQ=255)                                                        gaaatgaaatAATAAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGACGACAGAGTGTc       >  2:219791/1‑80 (MQ=255)                                                           atgaaataataaaataatgaaatGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGt   >  1:219799/1‑81 (MQ=255)                                                            tgaaataataaaataatgaaatGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTg  <  1:219772/81‑1 (MQ=255)                                                            tgaaataataaaataatgaaatGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTg  >  1:219793/1‑81 (MQ=255)                                                                     |                                                                        ATCGAGGAGGAGTAGCGCTACAAATTTGACGCTGGGTGAGAATCTGAAATTGATAGAAATGAAATAATGAAATAATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGAAATGATGACAGAGTGTCCAGTG  >  NC_000913/1198380‑1198519

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑