Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	111,731	T→C	E39G (GAA→GGA)	yacG ←	DNA gyrase inhibitor YacG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	111,731	0	T	C	100.0%	109.9 / NA	30	E39G (GAA→GGA)	yacG	DNA gyrase inhibitor YacG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/16);  total (14/16)

TTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGCCACTGCTGGGGATTCGTTTTTCTTCAGCAGCCCATTCTCCGAGGTCGATCAGCTGACAACGTTTGGAGCAAAATGGCCGAAACGGGCTGATTTCACCCCACACCACCGTTTT  >  NC_000913/111657‑111807                                                                           |                                                                             ttGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGCCACTGCTGGGGATTCGTTTTTCTTCAGCAGCCCAtcctcc                                                                          >  1:24314/1‑79 (MQ=255)    gTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGCCACTGCTGGGGATTCGTTTTTCTTCAGCAGCCCATCCTCCGAgg                                                                      <  1:24277/80‑1 (MQ=255)     ttcttcGCTCCAGTCATCGCTTTCGGAAAGATCGCCACTGCTGGGGATTCGTTTTTCTTCAGCAGCCCATCCTCCGAGGTc                                                                    <  1:24278/81‑1 (MQ=255)        ttcGCTCCAGTCATCGCTTTCGGAAAGATCGCCACTGCTGGGGATTCGTTTTTCTTCAGCAGCCCATCCTCCGAGGTCGAt                                                                 >  1:24315/1‑81 (MQ=255)          cGCTCCAGTCATCGCTTTCGGAAAGATCGCCACTGCTGGGGATTCGTTTTTCTTCAGCAGCCCATCCTCCGAGGTCGa                                                                  <  1:24280/78‑1 (MQ=255)          cGCTCCAGTCATCGCTTTCGGAAAGATCGCCACTGCTGGGGATTCGTTTTTCTTCAGCAGCCCATCCTCCGAGGTCGATc                                                                <  2:24279/80‑1 (MQ=255)           gCTCCAGTCATCGCTTTCGGAAAGATCGCCACTGCTGGGGATTCGTTTTTCTTCAGCAGCCCATCCTCCGAGGTCGATCAg                                                              >  2:24325/1‑81 (MQ=255)               cAGTCATCGCTTTCGGAAAGATCGCCACTGCTGGGGATTCGTTTTTCTTCAGCAGCCCATCCTCCGAGGTCGATCAGCTg                                                           <  2:24281/80‑1 (MQ=255)                 gTCATCGCTTTCGGAAAGATCGCCACTGCTGGGGATTCGTTTTTCTTCAGCAGCCCATCCTCCGAGGTCGATCAGCTGAc                                                         >  1:24318/1‑80 (MQ=255)                       gCTTTCGGAAAGATCGCCACTGCTGGGGATTCGTTTTTCTTCAGCAGCCCATCCTCCGAGGTCGATCAGCTGACAACGttt                                                  >  1:24316/1‑81 (MQ=255)                             ggAAAGATCGCCACTGCTGGGGATTCGTTTTTCTTCAGCAGCCCATCCTCCGAGGTCGATCAGCTGACAACGTTTGGAGc                                             <  2:24282/80‑1 (MQ=255)                              gAAAGATCGCCACTGCTGGGGATTCGTTTTTCTTCAGCAGCCCATCCTCCGAGGTCGATCAGCTGACAACGTTTGGAGCaa                                           <  1:24283/81‑1 (MQ=255)                                   aTCGCCACTGCTGGGGATTCGTTTTTCTTCAGCAGCCCATCCTCCGAGGTCGATCAGCTGACAACGTTTGGAGCAAAATgg                                      >  2:24319/1‑81 (MQ=255)                                    tCGCCACTGCTGGGGATTCGTTTTTCTTCAGCAGCCCATCCTCCGAGGTCGATCAGCTGACAACGTTTGGAGCAAAATGGc                                     <  1:24284/81‑1 (MQ=255)                                         aCTGCTGGGGATTCGTTTTTCTTCAGCAGCCCATCCTCCGAGGTCGATCAGCTGACAACGTTTGGAGCAAAATGGCCGaaa                                <  2:24285/81‑1 (MQ=255)                                          ctgctgGGGATTCGTTTTTCTTCAGCAGCCCATCCTCCGAGGTCGATCAGCTGACAACGTTTGGAGCAAAATGGCCGAAAc                               <  2:24286/81‑1 (MQ=255)                                                      cGTTTTTCTTCAGCAGCCCATCCTCCGAGGTCGATCAGCTGACAACGTTTGGAGCAAAATGGCCGAAACGGGCTGATTTCa                   >  2:24330/1‑81 (MQ=255)                                                        tttttCTTCAGCAGCCCATCCTCCGAGGTCGATCAGCTGACAACGTTTGGAGCAAAATGGCCGAAACGGGCTGATTTCAcc                 >  2:24337/1‑81 (MQ=255)                                                          tttCTTCAGCAGCCCATCCTCCGAGGTCGATCAGCTGACAACGTTTGGAGCAAAATGGCCGAAACGGGCTGATTTCAccc                >  1:24321/1‑80 (MQ=255)                                                            tcttcAGCAGCCCATCCTCCGAGGTCGATCAGCTGACAACGTTTGGAGCAAAATGGCCGAAACGGGCTGATTTCACCCca              >  2:24331/1‑80 (MQ=255)                                                             cttcAGCAGCCCATCCTCCGAGGTCGATCAGCTGACAACGTTTGGAGCAAAATGGCCGAAACGGGCTGATTTCACCCcaca            >  1:24332/1‑81 (MQ=255)                                                             cttcAGCAGCCCATCCTCCGAGGTCGATCAGCTGACAACGTTTGGAGCAAAATGGCCGAAACGGGCTGATTTCACCCcac             <  1:24287/80‑1 (MQ=255)                                                               tcAGCAGCCCATCCTCCGAGGTCGATCAGCTGACAACGTTTGGAGCAAAATGGCCGAAACGGGCTGATTTCACCCCacac           >  1:24329/1‑80 (MQ=255)                                                                cagcagCCCATCCTCCGAGGTCGATCAGCTGACAACGTTTGGAGCAAAATGGCCGAAACGGGCTGATTTCACCCCAcacca         >  1:24335/1‑81 (MQ=255)                                                                cagcagCCCATCCTCCGAGGTCGATCAGCTGACAACGTTTGGAGCAAAATGGCCGAAACGGGCTGATTTCACCCCAcacc          <  2:24288/80‑1 (MQ=255)                                                                 agcagcCCATCCTCCGAGGTCGATCAGCTGACAACGTTTGGAGCAAAATGGCCGAAACGGGCTGATTTCACCCCAcaccac        >  1:24326/1‑81 (MQ=255)                                                                 agcagcCCATCCTCCGAGGTCGATCAGCTGACAACGTTTGGAGCAAAATGGCCGAAACGGGCTGATTTCACCCCAcacca         <  1:24289/80‑1 (MQ=255)                                                                   cagcCCATCCTCCGAGGTCGATCAGCTGACAACGTTTGGAGCAAAATGGCCGAAACGGGCTGATTTCACCCCACACCACCg      <  1:24290/81‑1 (MQ=255)                                                                    agcCCATCCTCCGAGGTCGATCAGCTGACAACGTTTGGAGCAAAATGGCCGAAACGGGCTGATTTCACCCCACACCACCGt     >  1:24322/1‑81 (MQ=255)                                                                       ccATCCTCCGAGGTCGATCAGCTGACAACGTTTGGAGCAAAATGGCCGAAACGGGCTGATTTCACCCCACACCACCGtttt  <  2:24292/81‑1 (MQ=255)                                                                       ccATCCTCCGAGGTCGATCAGCTGACAACGTTTGGAGCAAAATGGCCGAAACGGGCTGATTTCACCCCACACCACCGtttt  <  1:24291/81‑1 (MQ=255)                                                                           |                                                                             TTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGCCACTGCTGGGGATTCGTTTTTCTTCAGCAGCCCATTCTCCGAGGTCGATCAGCTGACAACGTTTGGAGCAAAATGGCCGAAACGGGCTGATTTCACCCCACACCACCGTTTT  >  NC_000913/111657‑111807

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑