Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,277,223	G→C	A132A (GCC→GCG)	narX ←	sensory histidine kinase NarX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,277,223	0	G	C	100.0%	51.0 / NA	15	A132A (GCC→GCG)	narX	sensory histidine kinase NarX
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (7/8);  total (7/8)

ACCCGATGGACCAGTACCACTGTCTCGATGCGCATTTCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCGGCAACAAACTGGCTGACATCCGCTGACACCGTTTCTCGGTTTTGTGCACGCATCAGCGCAGGGATCA  >  NC_000913/1277146‑1277290                                                                              |                                                                    aCCCGATGGACCAGTACCACTGTCTCGATGCGCATTTCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGc                                                                   >  2:230234/1‑80 (MQ=255)         gACCAGTACCACTGTCTCGATGCGCATTTCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACTg                                                          >  1:230236/1‑81 (MQ=255)                aCCACTGTCTCGATGCGCATTTCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACa                                                   >  1:230241/1‑81 (MQ=255)                   aCTGTCTCGATGCGCATTTCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACATcc                                                <  1:230211/81‑1 (MQ=255)                    cTGTCTCGATGCGCATTTCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCg                                               <  1:230212/81‑1 (MQ=255)                      gTCTCGATGCGCATTTCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCt                                             >  1:230243/1‑81 (MQ=255)                      gTCTCGATGCGCATTTCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCt                                             >  1:230249/1‑81 (MQ=255)                           gATGCGCATTTCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGacac                                        >  2:230246/1‑81 (MQ=255)                           gATGCGCATTTCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGaca                                         <  1:230213/80‑1 (MQ=255)                                     tCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGACACCGTTTCTCg                               <  2:230214/80‑1 (MQ=255)                                         tggtgCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGACACCGTTTCTCGGtttt                          <  2:230215/81‑1 (MQ=255)                                                   aaaaCCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGACACCGTTTCTCGGTTTTGTGCACGCa                 <  1:230216/80‑1 (MQ=255)                                                   aaaaCCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGACACCGTTTCTCGGTTTTGTGCACGCAt                <  1:230217/81‑1 (MQ=255)                                                            tACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGACACCGTTTCTCGGTTTTGTGCACGCATCAGCGCAgg       >  2:230256/1‑81 (MQ=255)                                                                 gTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGACACCGTTTCTCGGTTTTGTGCACGCATCAGCGCAGGGATCa  <  2:230218/81‑1 (MQ=255)                                                                              |                                                                    ACCCGATGGACCAGTACCACTGTCTCGATGCGCATTTCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCGGCAACAAACTGGCTGACATCCGCTGACACCGTTTCTCGGTTTTGTGCACGCATCAGCGCAGGGATCA  >  NC_000913/1277146‑1277290

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑