Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,281,926	T→A	L688Q (CTG→CAG)	narG →	nitrate reductase A subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,281,926	0	T	A	100.0%	77.3 / NA	21	L688Q (CTG→CAG)	narG	nitrate reductase A subunit alpha
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (15/6);  total (15/6)

ACTAACCCGCTGACTATCGCTGGCGAAGCGGAAAAAGCCGGGATGAATCCGGTGGACTATACGGTGAAATCCCTGAAAGAGGGTTCCATCCGTTTTGCGGCAGAACAACCAGAAAACGGTAAAAACCACCCGCGCAACCTGTTCATCTGG  >  NC_000913/1281853‑1282002                                                                          |                                                                             aCTAACCCGCTGACTATCGCTGGCGAAGCGGAAAAAGCCGGGATGAATCCGGTGGACTATACGGTGAAATCCCAGAAagag                                                                       >  2:231010/1‑81 (MQ=255)   tAACCCGCTGACTATCGCTGGCGAAGCGGAAAAAGCCGGGATGAATCCGGTGGACTATACGGTGAAATCCCAGAAAGAggg                                                                     >  2:230984/1‑81 (MQ=255)   tAACCCGCTGACTATCGCTGGCGAAGCGGAAAAAGCCGGGATGAATCCGGTGGACTATACGGTGAAATCCCAGAAAGAggg                                                                     >  1:231002/1‑81 (MQ=255)       ccGCTGACTATCGCTGGCGAAGCGGAAAAAGCCGGGATGAATCCGGTGGACTATACGGTGAAATCCCAGAAAGAGGGTTc                                                                  >  1:231008/1‑80 (MQ=255)         gCTGACTATCGCTGGCGAAGCGGAAAAAGCCGGGATGAATCCGGTGGACTATACGGTGAAATCCCAGAAAGAGGGTTCCAt                                                               >  2:231019/1‑81 (MQ=255)            gACTATCGCTGGCGAAGCGGAAAAAGCCGGGATGAATCCGGTGGACTATACGGTGAAATCCCAGTAAGAGGGTTCCATCCg                                                            >  2:231011/1‑81 (MQ=255)              cTATCGCTGGCGAAGCGGAAAAAGCCGGGATGAATCCGGTGGACTATACGGTGAAATCCCAGAAAGAGGGTTCCATCCGtt                                                          <  1:230968/81‑1 (MQ=255)                 tCGCTGGCGAAGCGGAAAAAGCCGGGATGGATCCGGTGGACTATACGGTGAAATCCCAGAAAGAGGGTTCCATCCGttt                                                         <  2:230969/79‑1 (MQ=255)                       gCGAAGCGGAAAAAGCCGGGATGAATCCGGTGGACTATACGGTGAAATCCCAGAAAGAGGGTTCCATCCGTTTTGCgg                                                    >  1:230998/1‑78 (MQ=255)                          aaGCGGAAAAAGCCGGGATGAATCCGGTGGACTATACGGTGAAATCCCAGAAAGAGGGTTCCATCCGTTTTGCGGCAGaa                                               >  2:231009/1‑80 (MQ=255)                                    aGCCGGGATGAATCCGGTGGACTATACGGTGAAATCCCAGAAAGAGGGTTCCATCCGTTTTGCGGCAGAACAACCAGaaaa                                    >  1:231016/1‑81 (MQ=255)                                        gggATGAATCCGGTGGACTATACGGTGAAATCCCAGAAAGAGGGTTCCATCCGTTTTGCGGCAGAACAACCAGAAAACgg                                 >  2:231013/1‑80 (MQ=255)                                               aTCCGGTGGACTATACGGTGAAATCCCAGAAAGAGGGTTCCATCCGTTTTGCGGCAGAACAACCAGAAAACGGTAAAAac                          >  2:231003/1‑80 (MQ=255)                                                    gTGGACTATACGGTGAAATCCCAGAAAGAGGGTTCCATCCGTTTTGCGGCAGAACAACCAGAAAACGGTAAAAACCACCcg                    >  1:231001/1‑81 (MQ=255)                                                       gACTATACGGTGAAATCCCAGAAAGAGGGTTCCATCCGTTTTGCGGCAGAACAACCAGAAAACGGTAAAAACCACCcgcg                  <  1:230970/80‑1 (MQ=255)                                                            taCGGTGAAATCCCAGAAAGAGGGTTCCATCCGTTTTGCGGCAGAACAACCAGAAAACGGTAAAAACCACCCGCGCAACCt            >  1:231007/1‑81 (MQ=255)                                                               ggTGAAATCCCAGAAAGAGGGTTCCATCCGTTTTGCGGCAGAACAACCAGAAAACGGTAAAAACCACCCGCGCAACCTGtt         >  2:231004/1‑81 (MQ=255)                                                                gTGAAATCCCAGAAAGAGGGTTCCATCCGTTTTGCGGCAGAACAACCAGAAAACGGTAAAAACCACCCGCGCAACCTGTTc        <  1:230971/81‑1 (MQ=255)                                                                 tGAAATCCCAGAAAGAGGGTTCCATCCGTTTTGCGGCAGAACAACCAGAAAACGGTAAAAACCACCCGCGCAACCTGTTCa       >  2:231014/1‑81 (MQ=255)                                                                   aaaTCCCAGAAAGAGGGTTCCATCCGTTTTGCGGCAGAACAACCAGAAAACGGTAAAAACCACCCGCGCAACCTGTTCATc     <  2:230972/81‑1 (MQ=255)                                                                      tCCCAGAAAGAGGGTTCCATCCGTTTTGCGGCAGAACAACCAGAAAACGGTAAAAACCACCCGCGCAACCTGTTCATCTgg  <  1:230973/81‑1 (MQ=255)                                                                          |                                                                             ACTAACCCGCTGACTATCGCTGGCGAAGCGGAAAAAGCCGGGATGAATCCGGTGGACTATACGGTGAAATCCCTGAAAGAGGGTTCCATCCGTTTTGCGGCAGAACAACCAGAAAACGGTAAAAACCACCCGCGCAACCTGTTCATCTGG  >  NC_000913/1281853‑1282002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑