Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,284,681	T→C	S360P (TCT→CCT)	narH →	nitrate reductase A subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,284,681	0	T	C	100.0%	116.1 / NA	33	S360P (TCT→CCT)	narH	nitrate reductase A subunit beta
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/17);  total (16/17)

GCAATGGAATGGAAACTGGCGCTGCCGCTGCATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGTCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTATT  >  NC_000913/1284603‑1284758                                                                               |                                                                              gCAATGGAATGGAAACTGGCGCTGCCGCTGCATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGcct                                                                             >  1:231489/1‑81 (MQ=255)   aatggaatggAAACTGGCGCTGCCGCTGCATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTcc                                                                           >  2:231488/1‑81 (MQ=255)      ggaatggaaACTGGCGCTGCCGCTGCATCCGGAATATCGCACACTGACGATGGTCTGGTACGTGCCGCCTCTGCCTCCGAt                                                                        >  1:231487/1‑81 (MQ=255)        aatggaaACTGGCGCTGCCGCTGCATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTCCGATTc                                                                      <  1:231441/81‑1 (MQ=255)        aatggaaACTGGCGCTGCCGCTGCATCCGGAATATCGCACACTGCCGATGGCCTGGTACGTGCCGCCTCTGCCTCCGAtt                                                                       >  2:231485/1‑80 (MQ=255)         atggaaACTGGCGCTGCCGCTGCATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTCCGAtt                                                                       >  1:231479/1‑79 (MQ=255)           ggaaACTGGCGCTGCCGCTGCATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGt                                                                   >  2:231472/1‑81 (MQ=255)           ggaaACTGGCGCTGCCGCTGCATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGt                                                                   >  1:231484/1‑81 (MQ=255)            gaaACTGGCGCTGCCGCTGCATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGt                                                                   <  2:231443/80‑1 (MQ=255)            gaaACTGGCGCTGCCGCTGCATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTc                                                                  <  1:231442/81‑1 (MQ=255)              aaCTGGCGCTGCCGCTGCATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTCTg                                                                >  1:231482/1‑81 (MQ=255)                   gcgcTGCCGCTGCATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTCTgcagca                                                           <  1:231445/81‑1 (MQ=255)                   gcgcTGCCGCTGCATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTCTgcagc                                                            <  2:231444/80‑1 (MQ=255)                    cgctgccgctgcATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTCTGcagcag                                                          <  1:231446/81‑1 (MQ=255)                         ccgctgcATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTCTGCAGCAGAcgc                                                      <  2:231447/80‑1 (MQ=255)                            ctgcATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTCTGCAGCAGACGCGGGt                                                  >  1:231490/1‑81 (MQ=255)                               cATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTCTGCAGCAGACGCGGGTGAg                                               >  1:231496/1‑81 (MQ=255)                                aTCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGc                                              <  2:231448/81‑1 (MQ=255)                                aTCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGc                                              >  2:231493/1‑81 (MQ=255)                                    ggAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTggg                                          >  1:231478/1‑81 (MQ=255)                                     gAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGt                                         <  2:231449/81‑1 (MQ=255)                                          tCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCa                                     <  1:231450/80‑1 (MQ=255)                                           cGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAAc                                   >  2:231494/1‑81 (MQ=255)                                           cGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAAc                                   >  2:231481/1‑81 (MQ=255)                                                    ccGATGGTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAACGGCATTCTg                          <  2:231451/81‑1 (MQ=255)                                                      gATGGTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAACGGCATTCTGc                         >  1:231473/1‑80 (MQ=255)                                                      gATGGTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAACGGCATTCTGc                         >  2:231498/1‑80 (MQ=255)                                                           tCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAGACg                   <  2:231452/81‑1 (MQ=255)                                                               gTACGTGCCGCCTCTGCCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAGACGTc                 <  1:231454/79‑1 (MQ=255)                                                               gTACGTGCCGCCTCTGCCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAGACGTc                 <  2:231453/79‑1 (MQ=255)                                                                      ccgccTCTGCCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTg        <  1:231455/81‑1 (MQ=255)                                                                       cgccTCTGCCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGc       <  1:231456/81‑1 (MQ=255)                                                                         ccTCTGCCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGt     <  1:231457/81‑1 (MQ=255)                                                                            ctGCCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTAtt  >  2:231486/1‑81 (MQ=255)                                                                               |                                                                              GCAATGGAATGGAAACTGGCGCTGCCGCTGCATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGTCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTATT  >  NC_000913/1284603‑1284758

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑